Differences in Mast Cell Infiltration, E-cadherin, and Zonula Occludens-1 Expression Between Patients With Overactive Bladder and Interstitial Cystitis/Bladder Pain Syndrome

Liu, Hsin-Tzu; Shie, Jia-Heng; Chen, Sung-Ho; Wang, Yu-Syuan; Kuo, Hann‐Chorng

doi:10.1016/j.urology.2012.01.047

Cited by 106 publications

(125 citation statements)

References 28 publications

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“…We confirmed that the subepithelial location of mast cells in biopsies of patients with BPS/IC with Hunner lesions is different for BPS/IC with vs without Hunner lesions 3 and submucosal mast cell counts were similar in patients with BPS/IC without Hunner lesions and OAB. 5 Our detrusor mast cell count findings support previously published research on BPS/IC. 6 However, only 30% of the biopsies in our series met the ESSIC requirements of greater than 1 mm 2 detrusor muscle per biopsy 6 and counts were extrapolated to 1 mm 2 .…”

Section: Discussionsupporting

confidence: 91%

“…Groups have identified an increased mast cell count as a diagnostic criterion of BPS/IC 3,4 but an increased number of mast cells in the bladder wall was also reported in patients with OAB. 5 Different fixation/ staining techniques for mast cells and inconsistent criteria for patient selection and controls have resulted in conflicting results. 4 According to ESSIC guidelines a single criterion for detrusor mastocytosis (28 mast cells per mm 2 or greater) assigns a biopsy to the most severe biopsy category.…”

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confidence: 99%

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Are Mast Cells Still Good Biomarkers for Bladder Pain Syndrome/Interstitial Cystitis?

et al. 2015

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

Are Mast Cells Still Good Biomarkers for Bladder Pain Syndrome/Interstitial Cystitis?

et al. 2015

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“…Yet the mechanisms that regulate these events are essentially not known, decreased expression of ZO-1 in urotelium and diffusion of RR throughout the intercelluler space in this study showed that mobile phone EMWs can damage the intercellular junctional structures of rat urothelium. It is well known that abnormal E-cadherin and ZO-1 expression as well as increase in infiltration of mast cells in the urothelium are associated with IC/painful bladder syndrome (IC/PBS) [25,26].…”

Section: Discussionmentioning

confidence: 99%

The morphological and biochemical investigation of prenatal electromagnetic wave effects on urinary bladder in rats

Kiran¹,

Tok²,

Şehirli³

et al. 2017

Marmara Medical Journal

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The morphological and biochemical investigation of electromagnetic wave effects on urinary bladder in prenatal ratsPrenatal sıçan mesanesi üzerine elektromanyetik dalganın etkilerinin morfolojik ve biyokimyasal olarak incelenmesi ÖZ Amaçlar: Bu çalışmanın amacı, dijital mobil telefonlarından gelen ve en yüksek spesifik absorbsiyon oranı (Specific Absorbtion Rate-SAR) değeri 1,79W/kg olan dijital selüler sistem (DSS) 1800-MHz radyasyonunun erkek sıçan mesanesi üzerine etkilerini araştırmaktır.Gereç ve Yöntem: Sıçanlara mobil telefondan stand-by (Stand-by Fetal grup) ya da kesintisiz iletim modunda yayılan elektromagnetik dalga (EMD Fetal grup), günde 2 saat, embriyonik 14. günden doğuma kadar ya da doğumdan sonraki 60. güne kadar (Stand-by ve EMD grupları) uygulanmıştır. Deney gruplarındaki tüm hayvanların mesaneleri postnatal 60. günde eter anestezisi altında alınmış ve ışık ve elektron mikroskopik değerlendirmeler için hazırlanmıştır. Ürotelyumun bariyer fonkisyonu zonula okludens 1 ve E-kaderin immunhistokimyası ve transmisyon elektron mikroskobik inceleme için rutenyum kırmızısı boyaması ile değerlendirilmiştir. Oksidatif hasar biyokimyasal teknikler ile değerlendirilmiştir.Bulgular: Elektromagnetik dalga grubunda, ürotelyum dökülmeleri, mast hücre sayısında artış, sıkı bağlantılarda hasar ve interselüler alanda genişlemeler gözlenmiştir. Son olarak, kontrol grubu ile kıyaslandığında, tüm gruplarda malondialdehit seviyesinde yükselme ve glutatyon seviyesinde düşüş gözlenmiştir.Sonuç: Elektromagnetik dalga, maruz kalınan yoğunluk ve süreye paralel olarak, gelişim sürecindeki mesane üzerine olumsuz etki yapmaktadır. Bu değişiklikler mesanede enflamatuvar hastalıklara sebep olabilir.

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“…20 Tight junction protein ZO-1 serves as another important constituent to maintain the high-resistance urothelial barrier. 21 ZO-1 is regarded as multidomain scaffolding proteins that interlink the tight junction, a membrane-associated complex that controls the transport of ions, macromolecules, and immune cells. 22 However, for many patients with urological malignancy or extensive tissue and organ damage, organ biopsy cannot yield enough safe or sufficient cells for expansion and transplantation.…”

Section: Discussionmentioning

confidence: 99%

Tissue-engineered tubular substitutions for urinary diversion in a rabbit model

Meng

Liao

Yang

et al. 2015

Exp Biol Med (Maywood)

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Clinically, autologous gastrointestinal segments are traditionally used for urinary diversion. However, this procedure often causes many serious complications. Tissue engineering may provide an alternative treatment method in urinary diversion. This research aims to produce tissue-engineered tubular substitutions by using homologous adipose-derived stem cells, smooth muscle cells, and bladder acellular matrix in developing urinary diversion in a rabbit model. Adipose-derived stem cells and smooth muscle cells of rabbit were obtained and cultured in vitro. These cultured adipose-derived stem cells and smooth muscle cells were seeded onto the two sides of the bladder acellular matrix and then incubated for seven days. The cell-seeded matrix was used to build tissue-engineered tubular substitutions, which were then implanted and wrapped into the omentum in vivo for two weeks to promote angiogenesis. In the experimental group, the bladder of 20 rabbits was totally resected, and the above tissue-engineered tubular substitutions were used for urinary diversion. In the control group, bladder acellular matrix tubular substitutions with unseeded cells were implanted into the omentum and were used as urinary diversion on another five rabbits with the same process. The implants were harvested, and histological examination was conducted at 2, 4, 8, and 16 weeks after operation. Intravenous urography assessment was performed at 16 weeks postoperatively. All the rabbits were alive in the experimental group until they were sacrificed. Histological analysis of the construct displayed the presence of multilayer urothelial cells on the luminal side and organized smooth muscle tissue on the other side, and different diameters of neovascularization were clearly identified in the substitutions obtained. No leakage, stricture, or obstructions were noted with intravenous urography assessment. All the animals in the control group died within two weeks, and urine leakage, scar formation, and inflammation were detected through autopsy. This study demonstrates the feasibility of tissue-engineered tubular substitutions constructed using homologous adipose-derived stem cells, smooth muscle cells, and bladder acellular matrix for urinary diversion in a rabbit model.

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Differences in Mast Cell Infiltration, E-cadherin, and Zonula Occludens-1 Expression Between Patients With Overactive Bladder and Interstitial Cystitis/Bladder Pain Syndrome

Cited by 106 publications

References 28 publications

Are Mast Cells Still Good Biomarkers for Bladder Pain Syndrome/Interstitial Cystitis?

Are Mast Cells Still Good Biomarkers for Bladder Pain Syndrome/Interstitial Cystitis?

The morphological and biochemical investigation of prenatal electromagnetic wave effects on urinary bladder in rats

Tissue-engineered tubular substitutions for urinary diversion in a rabbit model

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