Differences in PAH tolerance between Capitella species: Underlying biochemical mechanisms

Bach, Lis; Palmqvist, Annemette; Rasmussen, Lene Juel; Forbes, Valery E.

doi:10.1016/j.aquatox.2005.06.002

Cited by 48 publications

(33 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability to metabolize PAHs varies highly among invertebrates (Rust et al 2004) and even between sibling species (Bach et al 2005). As no uptake, metabolism or depuration was measured in the current experiment it cannot be excluded that C. finmarchicus has a lower ability to metabolize PAHs than C. glacialis.…”

Section: Comparison Between Speciesmentioning

confidence: 74%

Experimental studies of reproduction and feeding for two Arctic-dwelling Calanus species exposed to crude oil

Jensen¹,

Carroll²

2010

Aquat. Biol.

View full text Add to dashboard Cite

Copepods of the genus Calanus are keystone species in the transfer of energy from lower to higher trophic levels of the Arctic/sub-Arctic food web. We performed experimental tests on the reproduction and feeding of Calanus spp. exposed to the water-soluble fraction (WSF) of crude oil. Fecal pellet and egg production were examined for females of C. glacialis exposed to WSF (16 EPA) concentrations of 10.4 µg l -1 (high treatment; HT), 3.6 µg l -1 (low treatment; LT) and 0 µg l -1(control treatment; CT). We observed no significant difference in cumulative egg or fecal pellet production. Egg hatching success was examined for 2 d after transferring eggs from treatment solutions to uncontaminated seawater. Hatching success was significantly lower in the HT compared to the CT. In a second experiment, feeding of C. finmarchicus was examined after exposure for 11 and 18 d to 7.0 (HT), 3.4 (LT) or 0 (CT) µg l -1 of WSF (16-EPA). Using algae cell concentrations as a proxy for feeding success, feeding was inhibited for C. finmarchicus specimens exposed to the HT of WSF compared to the CT. Our findings indicate that adult females of C. glacialis may withstand some exposure to crude oil components but the survival of offspring is negatively affected. Reduced feeding efficiency in C. finmarchicus exposed to high concentrations of WSF provides evidence that adult specimens are sensitive to exposure to crude oil. The study expands on the limited body of knowledge of potential changes to key life history traits of Arctic Calanus species resulting from exposure to chemical compounds in crude oil.

show abstract

Section: Comparison Between Speciesmentioning

confidence: 74%

Experimental studies of reproduction and feeding for two Arctic-dwelling Calanus species exposed to crude oil

Jensen¹,

Carroll²

2010

Aquat. Biol.

View full text Add to dashboard Cite

show abstract

“…This is also the first report of genotoxin-induced DNA damage in spermatozoa of a marine invertebrate. The only studies of between species differences in tolerance to environmental genotoxins in polychaetes have concentrated on differences in uptake, bioaccumulation and biotransformation of PAHs [33,34]. Antioxidant defence and repair capabilities of free cells (i.e.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic damage in polychaetes: A study of species and cell-type sensitivities

Lewis

Galloway

2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

The marine environment is becoming increasingly contaminated by environmental pollutants with the potential to damage DNA, with marine sediments acting as a sink for many of these contaminants. Understanding genotoxic responses in sediment dwelling marine organisms, such as polychaetes, is therefore of increasing importance. This study is an exploration of species specific and cell-specific differences in cell sensitivities to DNA damaging agents in polychaete worms, aimed at increasing fundamental knowledge of their responses to genotoxic damage. The sensitivities of coelomocytes from three polychaetes species of high ecological relevance; the lugworm Arenicola marina, the harbour ragworm Nereis diversicolor and the king ragworm Nereis virens to genotoxic damage are compared, and differences in sensitivities of their different coelomic cell types determined using the comet assay. Arenicola marina was found to be the most sensitive to genotoxic damage from the direct acting mutagen methyl methanesulfonate (MMS), and showed dose dependent responses to MMS and the polycyclic aromatic hydrocarbon benzo(a)pyrene. Significant differences in sensitivity of the different types of 2 coelomocyte were also measured. Eleocytes were more sensitive to DNA damage than amoebocytes in both Nereis virens and Nereis diversicolor. Arenicola marina spermatozoa showed significant DNA damage following in vitro exposure to MMS, but were less sensitive to DNA damage than coelomocytes. This investigation has clearly demonstrated that different cell types within the same species and different species within the Polychaeta show significantly different responses to genotoxic insult. These findings are discussed in terms of the relationship between cell function and sensitivity and their implications for the use of polychaetes in environmental genotoxicity studies.

show abstract

“…Generally, they thrive in organically enriched sediments, where they can reach densities of more than 200,000 individuals/m 2 (Sanders et al, 1980;Tsutsumi, 1987Tsutsumi, , 1990. Recently, interspecies variability with regard to tolerance to contaminants has been found within the species complex (Linke- Gamenick et al, 2000), which is associated with differences in biotransformation ability (Bach et al, 2005). The most opportunistic of the species so far examined, Capitella sp.…”

Section: Introductionmentioning

confidence: 99%

“…I is significantly more tolerant to Flu exposure than the slightly smaller Capitella sp. S (Linke- Gamenick et al, 2000), and this species is also a much more efficient biotransformer of accumulated Flu (Selck et al, 2003a;Bach et al, 2005). Though the former species may encounter genotoxic metabolites during the process of biotransformation, it is able to cope with the adverse effects of these by inducing DNA repair (Palmqvist et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The biotransformation process generally involves both cytochrome P-450 mixedfunction oxygenase, a step potentially producing reactive intermediate metabolites, and one or more subsequent en-zymatic conjugation processes, rendering the metabolite water-soluble and thereby more easily excretable (Lee and Singer, 1980). Large differences in biotransformation ability exist among different organisms (McElroy et al, 2000), and even closely related species can differ considerably in the degree to which they are capable of biotransforming PAHs (Bach et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation