Differences in photosynthetic responses of NADP-ME type C4 species to high light

Romanowska, Elżbieta; Buczyńska, Alicja; Wasilewska, Wioleta; Krupnik, Tomasz; Drożak, Anna; Rogowski, Paweł; Parys, Eugeniusz; Zienkiewicz, Maksymilian

doi:10.1007/s00425-016-2632-1

Cited by 14 publications

(5 citation statements)

References 71 publications

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“…This is validated herein, where 100 mM NaCl induced the activity of NADP-ME to be several folds higher; this may indicate light sensitization, however, under a saturated malate concentration. Therefore, the activity of NADP-ME would be complimented by light-induced redox changes, -SH/-S-S-, as well as under alkalinity [36]. Polymorphic gene expression for NADP-ME, as deduced through in-gel activity staining, also confirmed the light-induced NaCl sensitivity in the present experiment.…”

Section: Discussionsupporting

confidence: 78%

Regulation of NADP-Malic Enzyme Activity in Maize (Zea mays L.) under Salinity with Reference to Light and Darkness

Sarkar

Das

Pal

et al. 2023

Plants

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We revealed the functional characterization of C4-NADP-malic enzyme (NADP-ME), extracted and partially purified from maize (Zea mays L. cv. Kaveri 50). The leaf discs were previously activated under 1000–1200 µE m−2 s−1, incubated in bicarbonate (2.0 mM) solution, and subjected to salt stress (100 mM NaCl). Initially, salt stress was evident from the accumulations of proline, chlorophyll content, carbohydrate profile, and Hill activity influencing the C4 enzyme. Primarily, in illuminated tissues, the activity of the enzyme recorded a reduced trend through salinity irrespective of light and darkness compared to the control. On illumination, the kinetic parameters such as Vmax of the enzyme increased by 1.36-fold compared to in the dark under salinity whereas Km was decreased by 20% under the same condition. The extent of light induction was proportionate to limiting (0.01 mM) and saturated (4.0 mM) malate concentrations for enzyme activity. Moreover, the catalytic properties of the enzyme were also tested on concomitant responses to activator (citrate and succinate) and inhibitor (oxalate and pyruvate) residues. The sensitivity to light and dark effects was also tested for reducing agents such as dithiothreitol, suggesting the effect of the changes in redox on the regulatory properties of the enzyme. The ratio of enzyme activity under light and darkness in the presence or absence of a reducing agent was concomitantly increased with varying malate concentrations. At the molecular level, protein polymorphism of the enzyme represented minor variations in band intensities, however, not in numbers through salinity subjected to light and darkness. Therefore, salinity-induced changes in the decarboxylation reaction, evident by NADP-ME activity, may be based on the redox property of regulatory sites and sensitivity to light and darkness.

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Section: Discussionsupporting

confidence: 78%

Regulation of NADP-Malic Enzyme Activity in Maize (Zea mays L.) under Salinity with Reference to Light and Darkness

Sarkar

Das

Pal

et al. 2023

Plants

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“…In this way, the C4 photosynthetic metabolism operates a CO 2 -concentrating mechanism around Rubisco, which in turn significantly reduces the Rubisco oxygenase activity. In addition, BSC chloroplasts contain low level of PSII (Romanowska et al, 2017) and, although in BSC chloroplasts PSII contains all polypeptides involved in electron transport and oxygen evolution, it is largely inactive (Romanowska et al, 2008). In contrast, MC chloroplasts have higher PSII activity but practically absent Rubisco activity (Furbank and Foyer, 1988).…”

Section: Photoinhibition In C3 and C4 Speciesmentioning

confidence: 99%

Chlorophyll Fluorescence, Photoinhibition and Abiotic Stress: Does it Make Any Difference the Fact to Be a C3 or C4 Species?

2019

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Chlorophyll fluorescence analysis is one of the most powerful and widely used techniques to study the effect of stresses on the photosynthetic process. From the first utilization, the F v / F m ratio has been largely used as a sensitive indicator of plant photosynthetic performance. Decreases of this index are indicative of the reduction of photosystem II (PSII) efficiency, namely photoinhibition. In the last 20 years, application of chlorophyll fluorescence has been largely improved, and many other informative parameters have been established to detect PSII photochemical efficiency and the partitioning of light energy to alternative dissipative mechanisms (qE, energy-dependent quenching; qZ, zeaxanthin-dependent quenching and qI, photoinhibitory quenching; qH, sustained photoprotective antenna quenching; qM, quenching dependent to chloroplast movement; qT, light harvesting complexes II–I state-transition) such as the recently developed “photoprotective power” of non-photochemical quenching (pNPQ). This review reports a brief description of the main chlorophyll fluorescence parameters and a wide analysis of the current bibliography on the use of different parameters which are useful to detect events of PSII photoinhibition. In addition, in view of the inherent differences in morpho-anatomical, physiological and biochemical features between C3 and C4 metabolism, possible differences in terms of photoinhibition between C3 and C4 plant species under stress conditions are proposed. The attempt is to highlight the limits of their comparison in terms of susceptibility to photoinhibition and to propose direction of future research which, assisted by chlorophyll fluorescence, should improve the knowledge of the different sensitivity of C3 and C4 to abiotic stressors.

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“…us, PSII in BS agranal chloroplasts is not equivalent to PSII in the stromal membranes of C3 plants. (Romanowska et al, 2017). ese differences might be due to the imbalance in the excitation energy between the photosystems present in the M and BS cells and the differences in the penetration of light inside the leaves.…”

Section: Structure and Function Of Photosystem II In Agranal Maize Bu...mentioning

confidence: 99%

“…It was found that the non-radiative dissipation of energy in the studied plants was not dependent on carotenoid content, and the activity of the antioxidant enzymes and the malondialdehyde and H 2 O 2 levels were similar. is suggests that other photoprotective mechanism(s) might have been involved in NADP-ME species under high light stress (Romanowska et al, 2017).…”

Section: Structure and Function Of Photosystem II In Agranal Maize Bu...mentioning

confidence: 99%

Light-Dependent Reactions of Photosynthesis in Mesophyll and Bundle Sheath Chloroplasts of C4 Plant Maize. How Our Views Have Changed in Recent Years

Romanowska

Wasilewska-Dębowska

2022

Acta Soc Bot Pol

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Plants experience a range of light intensities and qualities in their environment. Leaves are subjected to spatial and temporal gradients in incident light, which has major consequences in the photosynthetic carbon assimilation. Plants acclimate to light by developing a range of mechanisms, from adjustments in leaf morphology to changes in the photosynthetic apparatus stoichiometry. In C4 plants, light intensity is a major limiting factor for photosynthesis at optimum temperatures. Under limiting light, it is not clear if all of factors (e.g., temperature, mineral nutrition, water supply) are co-limiting or if there is one primary limitation. Differences in light quality and intensity have a profound impact on C4 photosynthesis, where pathways require metabolic coordination of the mesophyll and bundle sheath cycles. Changes in the linear versus cyclic electron flux in maize (NADP-malic enzyme C4 subtype) in the mesophyll and bundle sheath chloroplasts in response to light may lead to an imbalance in the coordination of the C3 and C4 pathways. Additionally, the rearrangement of the thylakoid complexes of both types of chloroplasts in maize optimizes the light energy distribution between the mesophyll and bundle sheath cells and may also participate in energy dissipation. This review aims to highlight the changes in the understanding of the functions of photosystem II in maize bundle sheath chloroplasts and the role of super and megacomplexes in the thylakoids.

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Differences in photosynthetic responses of NADP-ME type C4 species to high light

Cited by 14 publications

References 71 publications

Regulation of NADP-Malic Enzyme Activity in Maize (Zea mays L.) under Salinity with Reference to Light and Darkness

Regulation of NADP-Malic Enzyme Activity in Maize (Zea mays L.) under Salinity with Reference to Light and Darkness

Chlorophyll Fluorescence, Photoinhibition and Abiotic Stress: Does it Make Any Difference the Fact to Be a C3 or C4 Species?

Light-Dependent Reactions of Photosynthesis in Mesophyll and Bundle Sheath Chloroplasts of C4 Plant Maize. How Our Views Have Changed in Recent Years

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