Differences in Soil Microbial Community Composition Between Suppressive and Root Rot-Conducive in Tobacco Fields

Ding, Ya-Ru; Chen, Yulan; Lin, Zhengquan; Tuo, Yangyang; Li, Hongli; Wang, Yan

doi:10.1007/s00284-020-02318-3

Cited by 26 publications

(18 citation statements)

References 19 publications

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“…The biological activity of these micro‐organisms may provide substrates for the synthesis of polysaccharides in L. barbarum , thereby promoting the accumulation of polysaccharides and total sugars in the fruit. norank_o_Gaiellales can improve abundance and activity of beneficial bacteria and inhibit root rot in soil (Ding et al, 2021). pH positively correlated with these genera and significantly affected sugar accumulation (Zheng et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Elucidating the interaction of rhizosphere bacteria and environmental factors in influencing active ingredient content of Lycium barbarum fruit in China

Liu

Wang

Lei

et al. 2022

Journal of Applied Microbiology

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Aims This study aimed to compare the differences in the bacterial community structure of Lycium barbarum rhizosphere and elucidate the contribution of rhizosphere bacteria to the active ingredients of L. barbarum fruit. Methods and Results This study investigated the soil and meteorological characteristics of L. barbarum rhizosphere during three growth stages across three production regions of China. High‐throughput sequencing showed significant differences in the bacterial community diversity of L. barbarum rhizosphere across the three production regions, and norank_o_Gaiellales, norank_f_Anaerolineaceae and norank_f_AKYG1722 were the highest in Ningxia. In addition, regression and path analysis revealed that pH, norank_o_Gaiellales and norank_f_AKYG1722 significantly promoted the accumulation of total sugar and flavonoids in L. barbarum fruit directly or indirectly. Soil organic matter (SOM), norank_f_Anaerolineaceae and humidity significantly promoted the accumulation of betaine. The average temperature during the growth stages, norank_f_AKYG1722, and norank_o_Gaiellales promoted the accumulation of polysaccharides. Conclusions The interaction between rhizosphere bacteria and environmental factors promoted the accumulation of active ingredients in L. barbarum fruits. Significance and Impact of the Study Our results provided insights to improve the quality of L. barbarum fruit.

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Section: Discussionmentioning

confidence: 99%

Elucidating the interaction of rhizosphere bacteria and environmental factors in influencing active ingredient content of Lycium barbarum fruit in China

Liu

Wang

Lei

et al. 2022

Journal of Applied Microbiology

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“…In addition, due to the current lack of a rapid and efficient method for the lily genetic transformation [ 15 ], LrWRKY3 was heterologously expressed in tobacco ( Nicotiana tabacum ) for functional verification. It is widely known that tobacco is an important model plant for rapid confirmation of gene functions, and F. oxysporum can rapidly cause Fusarium wilt in tobacco [ 16 ]. In addition, the LrWRKY3 function in defense response was explored in depth by transiently expressing its RNA interference (RNAi) fragment in L. regale scales.…”

Section: Introductionmentioning

confidence: 99%

Lilium regale Wilson WRKY3 modulates an antimicrobial peptide gene, LrDef1, during response to Fusarium oxysporum

et al. 2022

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Background WRKY transcription factors (TFs) play vital roles in plant growth and development, secondary metabolite synthesis, and response to biotic and abiotic stresses. In a previous transcriptome sequencing analysis of Lilium regale Wilson, we identified multiple WRKY TFs that respond to exogenous methyl jasmonate treatment and lily Fusarium wilt (Fusarium oxysporum). Results In the present study, the WRKY TF LrWRKY3 was further analyzed to reveal its function in defense response to F. oxysporum. The LrWRKY3 protein was localized in the plant cell nucleus, and LrWRKY3 transgenic tobacco lines showed higher resistance to F. oxysporum compared with wild-type (WT) tobacco. In addition, some genes related to jasmonic acid (JA) biosynthesis, salicylic acid (SA) signal transduction, and disease resistance had higher transcriptional levels in the LrWRKY3 transgenic tobacco lines than in the WT. On the contrary, L. regale scales transiently expressing LrWRKY3 RNA interference fragments showed higher sensitivity to F. oxysporum infection. Moreover, a F. oxysporum-induced defensin gene, Def1, was isolated from L. regale, and the recombinant protein LrDef1 isolated and purified from Escherichia coli possessed antifungal activity to several phytopathogens, including F. oxysporum. Furthermore, co-expression of LrWRKY3 and the LrDef1 promoter in tobacco enhanced the LrDef1 promoter-driven expression activity. Conclusions These results clearly indicate that LrWRKY3 is an important positive regulator in response to F. oxysporum infection, and one of its targets is the antimicrobial peptide gene LrDef1.

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“…The higher microbial diversity could provide stronger protection to plants from the invasion of adverse diseases. Recently, increasing studies have been devoted to disclosing the effect of plant disease on soil microorganism compositions, aiming to reveal the association between microbial community and the onset of plant disease and identify disease-related microbiomes (Cheng et al, 2019 ; Shi et al, 2019 ; Ding et al, 2021 ). The biocontrol of soilborne disease was based on the identification of key microbiomes related to plant diseases and the development of engineering bacteria to control the pathogenesis and progression of those diseases.…”

Section: Introductionmentioning

confidence: 99%

Response of Bacterial Community to the Occurrence of Clubroot Disease in Chinese Cabbage

Zong²,

Sun³

et al. 2022

Front. Microbiol.

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Clubroot disease is a common soilborne disease caused by Plasmodiophora brassicas Wor. and widely occurs in Chinese cabbage. Soil microorganisms play vital roles in the occurrence and development of plant diseases. The changes in the soil bacterial community could indicate the severity of plant disease and provide the basis for its control. This study focused on the bacterial community of the clubroot disease-infected soil–root system with different severity aiming to reveal the composition and structure of soil bacteria and identified potential biomarker bacteria of the clubroot disease. In the clubroot disease-infected soil, the bacterial community is mainly composed of Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Bacilli, Thermolrophilia, Bacteroidia, Gemmatimonadetes, Subgroup_6, Deltaproteobacteria, KD4-96, and some other classes, while the major bacterial classes in the infected roots were Oxyphotobacteria, Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, Bacilli, Bacteroidia, Saccharimonadia, Thermoleophilia, Clostridia, Chloroflexia, and some other classes. The severe clubroot disease soil–root system was found to possess a poorer bacterial richness, evenness, and better coverage. Additionally, a significant difference was observed in the structure of the bacterial community between the high-severity (HR) and healthy (LR) soil–root system. Bacillus asahii and Noccaea caerulescens were identified as the differential bacteria between the LR and HR soil and roots, respectively. pH was demonstrated as a vital factor that was significantly associated with the abundance of B. asahii and N. caerulescens. This study provides novel insight into the relationship between soil bacteria and the pathogen of clubroot disease in Chinese cabbage. The identification of resistant species provides candidates for the monitoring and biocontrol of the clubroot disease.

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Differences in Soil Microbial Community Composition Between Suppressive and Root Rot-Conducive in Tobacco Fields

Cited by 26 publications

References 19 publications

Elucidating the interaction of rhizosphere bacteria and environmental factors in influencing active ingredient content of Lycium barbarum fruit in China

Elucidating the interaction of rhizosphere bacteria and environmental factors in influencing active ingredient content of Lycium barbarum fruit in China

Lilium regale Wilson WRKY3 modulates an antimicrobial peptide gene, LrDef1, during response to Fusarium oxysporum

Response of Bacterial Community to the Occurrence of Clubroot Disease in Chinese Cabbage

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