Differences in Surface Marker Expression and Chondrogenic Potential among Various Tissue-Derived Mesenchymal Cells from Elderly Patients with Osteoarthritis

Alegre-Aguarón, Elena; Desportes, Paula; García-Álvarez, F; Castiella, Tomás; Larrad, Luis; Martínez‐Lorenzo, María José

doi:10.1159/000334400

Cited by 30 publications

(25 citation statements)

References 119 publications

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“…Therefore, insights into the multipotency and efficiency of differentiation into different lineages of MSCs from IFP of OA versus healthy individuals are lacking. Comparison between MSCs from IFP, sc adipose tissue, bone marrow, and synovial fluid of patients with OA has indicated that IFP MSCs are most similar in phenotype (that is, surface marker expression) to sc adipose tissue MSCs but have proliferative and chondrogenic differentiation capacities most similar to bone marrow MSCs [54]. However, in a different study, IFP MSCs displayed enhanced chondrogenic and adipogenic capacity when compared with normal bone marrow MSCs [55], although differences in age between the OA and healthy population could account for this difference.…”

Section: Infrapatellar Fat Pad As a Source Of Mesenchymal Stem Cellsmentioning

confidence: 99%

An emerging player in knee osteoarthritis: the infrapatellar fat pad

2013

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The role of inflammation in the development, progression, and clinical features of osteoarthritis has become an area of intense research in recent years. This led to the recognition of synovitis as an important source of inflammation in the joint and indicated that synovitis is intimately associated with pain and osteoarthritis progression. In this review, we discuss another emerging source of inflammation that could play a role in disease development/progression: the infrapatellar fat pad (IFP). The aim of this review is to offer a comprehensive view of the pathology of IFP as obtained from magnetic resonance studies, along with its characterization at both the cellular and the molecular level. Furthermore, we discuss the possible function of this organ in the pathological processes in the knee by summarizing the knowledge regarding the interactions between IFP and other joint tissues and discussing the pro- versus anti-inflammatory functions this tissue could have. We hope that this review will offer an overview of all published data regarding the IFP and will indicate novel directions for future research.

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Section: Infrapatellar Fat Pad As a Source Of Mesenchymal Stem Cellsmentioning

confidence: 99%

An emerging player in knee osteoarthritis: the infrapatellar fat pad

2013

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“…MSCs isolated from human umbilical cord Wharton's jelly by the collagenase/trypsin method are enriched in expression of C-kit and Oct-4 [53]. MSCs from bone marrow and Hoff's fat pad show a high potential to differentiate into chondrocytes whereas MSCs from subcutaneous fat demonstrate a poor potential for chondrogenesis [54]. Rabbit and sheep MSCs were able to differentiate into chondrocytic lineages much more easily than human MSCs [45].…”

Section: Phenotypically and Functionally Heterogeneous Mscsmentioning

confidence: 99%

The uncertain role of unmodified mesenchymal stem cells in tumor progression: what master switch?

Zhang

Xiang

2013

Stem Cell Res Ther

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Mesenchymal stem cells (MSCs) are emerging as promising gene vectors for cancer therapy because of their unique characteristics, including the ease of their expansion and genetic modification and their remarkable tumor-tropic properties. However, there remains a concern that MSCs may promote cancer progression. Surprisingly, there are conflicting reports within the literature describing both the promotion and inhibition of cancer progression by MSCs. The reasons for this discrepancy are still unknown. The surface markers, differentiation ability, and tumorigenic roles of MSCs, as well as their effect on immunoregulation, produce heterogeneity. In this review, we describe the heterogeneity of MSCs by the species from which they are derived, the methodology for their isolation and the context of their interactions with cancer cells. The conflicting roles of MSCs in tumor progression may be attributable to the bimodal effect of unmodified MSCs on immunoregulation. MSCs have been reported to suppress T-cell function and inhibit graft-versus-host disease (GVHD). On the other hand, MSCs elicit the graft-versus-tumor (GVT) effect in some cases. Selective allodepletion may be used to dissociate GVHD from the GVT effect. Understanding the conditions that balance GVHD and the GVT effect of MSCs may be crucial to advance cancer therapy research with respect to MSCs.

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“…The proliferative and differentiation capacities of synovial fluidderived progenitor cells have varied between studies [9][10][11][12][13], but in all cases, these cells were capable of chondrogenesis. However, the specific chondrogenic lineage of synovial fluidderived chondroprogenitors (SF-CP) has not been defined.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

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Background: Progenitor cells exist in most tissues and body fluids. Synovial fluid chondroprogenitor cells have been described in several species; however, the specific phenotypic characteristics of these cells have not been defined. This study addressed the impacts of joint location and donor age variation on synovial fluid chondroprogenitor numbers, and determined whether synovial fluid chondroprogenitors express hypertrophic characteristics or a non-endochondral phenotype in chondrogenic culture. Methods: Synovial fluid was aspirated from the joints of healthy adult horses, and cells in the aspirates were expanded through two monolayer passages. The number of colony-forming units (CFU) in each aspirate was assessed after 14 days. Population doublings (PD) and PD times were calculated during the subsequent two passages. Passage 3 cells were used to determine osteogenic and adipogenic capacities, or were transferred to pellet cultures in chondrogenic medium containing TGF-β1 or BMP-2. Bone marrowderived MSCs and primary articular chondrocytes were cultured under similar conditions to provide reference values for chondrogenesis. Chondrogenesis was assessed by measuring collagen type II protein and sulfated glycosaminoglycan secretion, expression of chondrogenic mRNAs, and induction of ALP activity. Results: CFU counts varied considerably, but the majority of aspirates contained <50 CFU/ml. PDs were consistently between 2.0 and 3.0 during both passages. PD times varied from 0.2-0.4 days. Proliferation was not significantly influenced by anatomical location or donor age. Equine synovial fluid progenitors expressed osteogenic, adipogenic and chondrogenic phenotypes under appropriate conditions. Under chondrogenic conditions, synovial fluid cells increased collagen and aggrecan mRNA expression to levels comparable to bone marrow MSCs, although collagen type II protein secretion was less than half that of articular chondrocytes. In contrast to bone marrow MSCs, synovial fluid chondroprogenitors did not express collagen type X or increase ALP activity in response to TGF-β1 or BMP-2. Consistent with these observations, Runx2 and Mef2C expression in synovial fluid chondroprogenitors was 20-40 fold lower than in bone marrow MSCs. Conclusions: Synovial fluid chondroprogenitors are capable of robust non-hypertrophic chondrogenesis, whether stimulated by TGF-β1 or by BMP-2. These results indicate that synovial fluid cells are phenotypically suitable for articular cartilage repair/regeneration, although strategies to accelerate cell proliferation and synthesize a functional cartilage matrix in vivo will be required for clinically feasible cellbased therapies.

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Differences in Surface Marker Expression and Chondrogenic Potential among Various Tissue-Derived Mesenchymal Cells from Elderly Patients with Osteoarthritis

Cited by 30 publications

References 119 publications

An emerging player in knee osteoarthritis: the infrapatellar fat pad

An emerging player in knee osteoarthritis: the infrapatellar fat pad

The uncertain role of unmodified mesenchymal stem cells in tumor progression: what master switch?

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

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