Differences in the adhesive properties of Neisseria meningitidis for human buccal epithelial cells and erythrocytes

Trust, Trevor J.; Gillespie, R M; Bhatti, A. R.; White, L A

doi:10.1128/iai.41.1.106-113.1983

Cited by 38 publications

(17 citation statements)

References 27 publications

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“…According to our results obtained with purified PilC2 protein or piliated gonococci, the potential host cell receptor for pili/PilC is a protein and sugar components do not seem to be involved in the interaction. These data are consistent with observations from other groups who found that a eukaryotic protein or glycoprotein could be a potential pilus receptor, whereas sugar structures are not involved in binding (Watt and Ward, 1977;Trust et al, 1983;Jonsson et al, 1994). In contrast, Buchanan and colleagues could inhibit the adherence of piliated gonococci by host cell treatment with various gangliosides or exoglycosidases (Buchanan et al, 1978).…”

Section: Discussionsupporting

confidence: 89%

The PilC adhesin of the Neisseria type IV pilus – binding specificities and new insights into the nature of the host cell receptor

Kirchner

Meyer

2005

Molecular Microbiology

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SummaryType IV pili of Neisseria gonorrhoeae and Neisseria meningitidis mediate the first contact to human mucosal epithelial cells, an interaction which is also critical for the interaction with vascular endothelial cells. The PilC proteins have been characterized as the principal pilus-associated adhesin. Here we show that PilC2 exhibits a defined cell and tissue tropism, as it binds to human epithelial and endothelial cell lines, but not to human T cells or fibroblasts. Piliated gonococci and PilC2 exhibit similar patterns of binding to human epithelial and endothelial cells, supporting the function of PilC as the key pilus adhesin. Although CD46 has previously been suggested to be a pilus receptor, several observations indicate that neisserial type IV pili and the pilus adhesin PilC2 interact with epithelial cells in a CD46 independent manner. Biochemical approaches were used to characterize the nature of host cell factors mediating binding of piliated gonococci and PilC2 protein. Our data indicate that the putative host cell receptor for gonococcal pili and the PilC2 pilus adhesin is a surface protein. Glycostructures were found to not be involved in binding. Moreover, we observed the uptake of purified PilC2 protein together with its receptor via receptor-mediated endocytosis and subsequent receptor re-exposure on the cell surface. Our data support the existence of a specific pilus receptor and provide intriguing information on the nature of the receptor.

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Section: Discussionsupporting

confidence: 89%

The PilC adhesin of the Neisseria type IV pilus – binding specificities and new insights into the nature of the host cell receptor

Kirchner

Meyer

2005

Molecular Microbiology

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“…CD147 is expressed on different cell types, such as erythrocytes and epithelial cells. Pilus-dependent interactions between meningococci and erythrocytes, causing hemagglutination, have been documented in vitro 35,36 , and meningococci have been described crossing polarized epithelia, indicating that meningococcus might interact with CD147 in a variety of cell types. Notably, several viruses, including HIV-1, severe acute respiratory syndrome coronavirus 37 and measles virus 38 , as well as the bacterial pathogen Listeria monocytogenes 39 , also target CD147 to adhere to and/or invade epithelial cells, indicating that CD147 constitutes an evolutionarily conserved efficient target for pathogens to infect tissues and spread within organisms.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization

Bernard

Simpson

Join‐Lambert

et al. 2014

Nat Med

138

140

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Neisseria meningitidis is a cause of meningitis epidemics worldwide and of rapidly progressing fatal septic shock. A crucial step in the pathogenesis of invasive meningococcal infections is the adhesion of bloodborne meningococci to both peripheral and brain endothelia, leading to major vascular dysfunction. Initial adhesion of pathogenic strains to endothelial cells relies on meningococcal type IV pili, but the endothelial receptor for bacterial adhesion remains unknown. Here, we report that the immunoglobulin superfamily member CD147 (also called extracellular matrix metalloproteinase inducer (EMMPRIN) or Basigin) is a critical host receptor for the meningococcal pilus components PilE and PilV. Interfering with this interaction potently inhibited the primary attachment of meningococci to human endothelial cells in vitro and prevented colonization of vessels in human brain tissue explants ex vivo and in humanized mice in vivo. These findings establish the molecular events by which meningococci target human endothelia, and they open new perspectives for treatment and prevention of meningococcus-induced vascular dysfunctions.

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“…Pili (fimbriae), the filamentous protein structures which radiate from the surface of the bacteria, have been shown to mediate interactions of both N. meningitidis and N. gonorrhoeae to mucosal epithelial celis (Craven and Frasch, 1978;McGee and Stephens. 1984;Virji and Everson, 1981;Ward and Watt, 1975) and to erythrocytes (Trust et al, 1983). In addition, increased toxicity of the piliated organisms for the epithelial cells has also been reported (Stephens ef a/., 1988a).…”

Section: Introductionmentioning

confidence: 97%

The role of pili in the interactions of pathogenic Neisseria with cultured human endothelial cells

Virji

Käyhty

Ferguson

et al. 1991

Molecular Microbiology

211

196

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The influence of the two surface structures of Neisseria meningitidis, capsule and pili, in bacterial interactions with human endothelial cells was investigated. Increased association correlated with the presence of pili on bacteria while capsule type had no apparent effect. Strains expressing both Class I and Class II pili associated with endothelial cells in significantly larger numbers compared with the non-piliated variants of the same strains (greater than 10x). Variants of Neisseria gonorrhoeae strain P9 expressing antigenically distinct pili also associated with endothelial cells in larger numbers (greater than 30x) compared with the non-piliated variant. Electron microscopic studies confirmed these data and showed that gonococci were internalized more frequently compared with meningococci. One consequence of increased association was an increase in the cytopathic effect of bacteria on the target cells.

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Differences in the adhesive properties of Neisseria meningitidis for human buccal epithelial cells and erythrocytes

Cited by 38 publications

References 27 publications

The PilC adhesin of the Neisseria type IV pilus – binding specificities and new insights into the nature of the host cell receptor

The PilC adhesin of the Neisseria type IV pilus – binding specificities and new insights into the nature of the host cell receptor

Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization

The role of pili in the interactions of pathogenic Neisseria with cultured human endothelial cells

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