Differences in the antibacterial activity of nisin and bovicin HC5 against Salmonella Typhimurium under different temperature and pH conditions

Abstract

Nisin and bovicin HC5 have the potential to inhibit the growth of Salmonella, but environmental conditions should be considered to establish optimal conditions for its application.

Strategies for the use of bacteriocins in Gram-negative bacteria: relevance in food microbiology

“…However, several factors can alter the sensibility of target cells, including the manner of administration of the chelating agent, the time of treatment and the environmental conditions of treatment (Boziaris and Adams 1999;Phillips and Duggan 2001;Prudêncio et al 2015). The inhibition of microbial growth appears to be a time-dependent process, and the method of application can be a critical factor in obtaining the desired effect, it is recommended the simultaneous use of the bacteriocin and the chelating agent (Boziaris and Adams 1999;Phillips and Duggan 2001;Stevens et al 1991).…”

“…The activity of the chelating agent is also influenced by pH: at low pH, a large proportion of the carboxylate group is in its nonionized form, which is not a particularly effective electron donor and the EDTA-metal complex is less stable. Therefore, the combination of EDTA with bacteriocins is more effective at near-neutral pH, although several peptides, such as nisin and bovicin HC5, are more efficient to low pH value (Ananou et al 2005;Boziaris and Adams 1999;Houlihan et al 2004;Lappe et al 2009;Norhana et al 2012;Prudêncio et al 2015).…”

“…These alterations may permeabilize the cell to bacteriocin, allowing the nisin to act on S. Typhimurium and E. coli at refrigeration temperatures (Elliason and Tatini 1999;Prudêncio et al 2015). The chilling process only allows the effective sensitization of Gram-negative bacteria to the action of bacteriocins, when the temperature drops rapidly, because there is not enough time for the reorganization of the outer membrane, altering its permeability.…”

Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA

“…2 0 07 b ) and Alicyclobacillus acidoterrestris (de Carvalho et al 2008) has already been demonstrated. Activity on Gramnegative bacteria was also recently demonstrated (Prudêncio et al 2014(Prudêncio et al , 2015a.…”

“…This resistance is due to the outer membrane, which functions as a barrier, rendering the organism less permeable (Chalón et al 2012;Gálvez et al 2014;Gyawali and Ibrahim 2014). However, this resistance can be reduced with the use of compounds or treatments that destabilize the outer membrane, such as chelating agents, plant essential oils, freezing, heating, high pressure processing and pulsed electric fields (Boziaris and Adams 1999;Cobo Molinos et al 2008;Prudêncio et al 2014Prudêncio et al , 2015a.…”