Differences in the antibody response and survival of genetically different varieties of common carp (Cyprinus carpio L.) vaccinated with a commercial Aeromonas salmonicida/A. hydrophila vaccine and challenged with A. hydrophila

Abstract

Males of two strains of carp, wild Duna (D), and inbred Szarvas 22 (22), were selected for high and low stress response. Two purebreds of D and 22, from randomly chosen parents and four crosses, 22 x 22-L (low stress response), 22 x 22-H (high stress response), 22 x D-L (low stress response) and 22 x D-H (high stress response) from selected stress response parents were produced and vaccinated with a commercial Aeromonas salmonicida/Aeromonas hydrophila vaccine and their circulating antibody response evaluated 1, 3, 5, and 7 weeks post-vaccination by ELISA. Significantly higher titres of circulatory antibodies against A. hydrophila were found in the families 22 and cross 22 x 22-L compared to other groups. The development of circulatory antibodies against A. hydrophila in all crosses having at least one D parent was low and remained low throughout the experiment. The level of circulatory antibodies against atypical A. salmonicida in the inbred strain increased following a booster vaccination with the highest values measured in inbred strain 22 and cross 22 x 22 L. The different varieties of carp had different levels of survival against experimental challenge with A. hydrophila. The greatest survival was obtained in strain 22 and cross 22 x 22-L, while ~90% of D wild carp and cross 22 x D (independent of their stress response) died. Survival results correlated well with the antibody response of the different groups: 22 and 22 x 22-L had the highest antibody titres against A. hydrophila and the greatest level of survival.

Immune responses of resistant and sensitive common carp families following experimental challenge with Aeromonas hydrophila

“…Genetically different common carp families had different specific antibody levels after vaccination with a commercial A. salmonicida/A. hydrophila vaccine, and the level of specific antibodies correlated well with the survival following a challenge with A. hydrophila [40]. Isogenic hybrid common carp lines, highly susceptible to infection of Trypanoplasma borreli (a haemoflagellate parasite of carp) did not produce specific antibodies after challenge with the parasite, whereas resistant outbred carps showed a significant increase of specific antibodies [24].…”

Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila

“…Swelling of tissues, dropsy, red sores, necrosis, ulceration, and hemorrhagic septicemia are typical symptoms of MAS [3,4]. Many fish species can be affected by MAS, including tilapia [5,6], catfish [7,8], goldfish [9,10], common carp [11,12], and eel [13]. As an opportunistic pathogen, A. hydrophila caused outbreaks in fish farms with high mortality rates [14e16].…”

Transcriptional profiles of multiple genes in the anterior kidney of channel catfish vaccinated with an attenuated Aeromonas hydrophila

“…The symptoms of A. hydrophila infection include swelling of tissues, dropsy, red sores, necrosis, ulceration, and hemorrhagic septicemia [3,4]. Fish species affected by MAS include tilapia [5,6], catfish [7,8], goldfish [9,10], common carp [11,12], and eel [13]. Although usually considered as a secondary pathogen associated with disease outbreaks, A. hydrophila could also become a primary pathogen, causing outbreaks in fish farms with high mortality rates, resulting in severe economic losses to the aquaculture industry worldwide [14e16].…”