Transcriptional profiles of multiple genes in the anterior kidney of channel catfish vaccinated with an attenuated Aeromonas hydrophila

Mu, Xingjiang; Pridgeon, Julia W.; Klesius, Phillip H.

doi:10.1016/j.fsi.2011.10.011

Cited by 30 publications

(21 citation statements)

References 53 publications

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“…Anterior kidney tissues were collected (in triplicate) aseptically in RNAlater (Sigma-Aldrich Chemicals Co., USA) by sacrificing three fish from each group (one from each replicate) at 0 h (before challenge) and 12, 24, 48, 72 and 120 h post-challenge, based on our previous observations and earlier reports in channel catfish, regarding the presence of an increased load of bacteria at around 12 h or thereafter, causing major damage to the anterior kidney. They were kept at -80 °C for further study (MAHAPATRA et al, 2008;MU et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

et al. 2017

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Although vaccination and post-vaccination studies have gained considerable importance in the field of aquaculture diseases, little is understood about the mechanism of immune-protection in vaccinated fish following infection. In the present study, an attempt was made to discover the mechanism of protection rendered by a modified adjuvant-based recombinant outer membrane protein R (rOmpR) vaccine, in rohu, Labeo rohita (an important Indian major carp species) following a challenge with Aeromonas hydrophila. For this, expression analysis of an array of immune molecules (IgM, complement factor 3, interleukin 1β, interleukin 6, tumor necrosis factor α, interleukin 15, MHC I and MHC II) was carried out in the anterior kidney tissues of vaccinated fish, at 0, 12, 24, 48, 72 and 120 h post-challenge. Higher transcript levels of IgM, MHC I and MHC II were evident in the mineral oil-based OmpR vaccinated and modified adjuvant-based OmpR-vaccinated groups, at different time points post-challenge, including the non-challenged fish. Early onset of pro-inflammatory cytokines and expression of most of the immune genes was more pronounced in vaccinated fish at different time points post-challenge, thus indicating a better immune response in these fish. This study indicated the activation of both humoral and cell mediated immunity in vaccinated rohu following a challenge with A. hydrophila.

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Section: Methodsmentioning

confidence: 99%

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

et al. 2017

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“…Immunity of fish to bacterial infections is largely mediated by cellular immune responses with humoral antibodies having a secondary role [20,21]. It was reported that live attenuated A. hydrophila induced the upregulation of multiple immune genes in channel catfish [22]. In addition, infection with A. hydrophila induced peak up-regulation of several genes at 6 h post injection (hpi) [22].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila

Pridgeon

Klesius

2013

Fish & Shellfish Immunology

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To determine whether transcriptional levels of channel catfish (Ictalurus punctatus) genes are differentially regulated between a first infection with Aeromonas hydrophila and a re-infection, suppression subtractive hybridization (SSH) was performed in this study using anterior kidney cDNA after the re-infection as tester. Of the 96 clones isolated from the SSH library, 28 unique expressed sequence tags (ESTs) were obtained, of which eight were confirmed to be slightly but significantly (P < 0.05) more up-regulated by the re-infection at 6 h post infection (hpi). Expression kinetics studies at 3, 6, 12, 24, and 48 hpi revealed that the eight ESTs were significantly (P = 0.016) more up-regulated by the first infection, with a major peak at 3 hpi. A total of 96 genes reported in literature to be up-regulated by bacterial infections were selected and subjected to expression analysis at 3 hpi. Of the 96 selected genes, 19 were found to be significantly (P < 0.05) induced by A. hydrophila after the first infection and the re-infection. The 19 genes belonged to the following five main categories: 1) toll-like receptor (TLR2, TLR3, TLR5, TLR21); 2) antimicrobial peptide (NK-lysin type 1, NK-lysin type 2, NK-lysin type 3, cathepsin D, transferrin, hepcidin); 3) cytokine or chemokine (interleukin-1β, interleukin-10, tumor necrosis factor α, chemokine CXCL-10); 4) signaling proteins (cadherin EGF LAG seven-pass G-type receptor 1, very large inducible GTPase 1, arginine deiminase type 2, lymphokine-activated killer T-cell originated protein kinase); 5) lysozyme (lysozyme c). Overall, the total 27 genes (8 ESTs plus the 19 selected genes) were significantly (P < 0.001) more induced by the first infection. Peaked expression of lysozyme c and serum lysozyme activity after the first infection were seen at 24 hpi, whereas that after the re-infection were seen at 12 hpi, suggesting that both innate and adaptive immunity were involved in the defense against the re-infection of A. hydrophila.

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“…Aeromonas hydrophila, a Gram-negative motile bacillus widely distributed in aquatic environments, it causes motile aeromonad septicemia (MAS), which results in great economic losses in freshwater fish farming worldwide [23,24]. It has caused a high fish mortality, resulting in reduced production and considerable economic losses in the aquaculture of grass carp in China [25].…”

Section: Introductionmentioning

confidence: 99%

Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection

Xu¹,

Shen²,

Fu³

et al. 2012

Fish & Shellfish Immunology

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Transcriptional profiles of multiple genes in the anterior kidney of channel catfish vaccinated with an attenuated Aeromonas hydrophila

Cited by 30 publications

References 53 publications

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila

Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection

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Transcriptional profiles of multiple genes in the anterior kidney of channel catfish vaccinated with an attenuated Aeromonas hydrophila

Cited by 30 publications

References 53 publications

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila

Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection

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Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3