Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection

Xu, Xiaoyan; Shen, Yubang; Fu, Jianjun; Liu, Feng; Guo, Shi-Zhao; Yang, Xiaomeng; Li, Jiale

doi:10.1016/j.fsi.2012.05.015

Cited by 21 publications

(10 citation statements)

References 61 publications

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“…While it was thought to be an opportunistic pathogen in humans, a growing number of intestinal and extra-intestinal cases of disease indicate that Aeromonas hydrophila is an emergent human pathogen, irrespective of the immunological aspects of the host [ 2 ]. Aeromonas hydrophila is a Gram-negative motile bacillus that causes motile aeromonad septicaemia [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream ( Megalobrama amblycephala )

Hu¹,

Tang²,

Lu³

et al. 2017

cejoi

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Aeromonas hydrophila is the main reason of epidemic septicaemia for freshwater fish. In the present study, the effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream (Megalobrama amblycephala) was studied. After Aeromonas hydrophila challenge, lysozyme activity was significantly increased at 4 h, 1 d, 3 d, 5 d, 14 d and 21 d. An increased level of lysozyme activity indicated a natural protective mechanism in fish. The significant increases of superoxide dismutase activity and catalase activity in treatment group were detected at 4 h, 1 d, 3 d, 5 d, 14 d and 21 d after Aeromonas hydrophila challenge. Increase in the superoxide anion and H2O2 is considered to be beneficial for self-protection from disease. Acid phosphatase activity increased significantly at 1 d, 3 d and 5 d after Aeromonas hydrophila challenge. Alkaline phosphatase activity in treatment group showed significant increase at 4 h, 1 d, 3 d, 5 d, 14 d and 21 d compared to control group. Increased phosphatase activity indicates higher breakdown of the energy reserve, which is utilized for the growth and survival of fish. These results revealed that the non-specific immunity of fish played an important role in self-protection after pathogens infection.

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Section: Introductionmentioning

confidence: 99%

The effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream ( Megalobrama amblycephala )

Hu¹,

Tang²,

Lu³

et al. 2017

cejoi

View full text Add to dashboard Cite

show abstract

“…A . hydrophila is a Gram-negative motile bacillus that causes motile aeromonad septicemia [ 11 ]. Therefore, effective measures are required to protect against infections with this pathogenic micro-organism in aquatic animals.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

Dang

Shen

et al. 2016

PLoS ONE

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The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs). 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens.

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“…A. hydrophila is a Gram-negative motile bacillus widely distributed in aquatic environments. It causes motile aeromonad septicemia (MAS), which results in great economic losses in worldwide freshwater fish farming [12] . Thus, more effective measures against A. hydrophila infection in fish are needed.…”

Section: Introductionmentioning

confidence: 99%

De novo Assembly of the Grass Carp Ctenopharyngodon idella Transcriptome to Identify miRNA Targets Associated with Motile Aeromonad Septicemia

Shen

et al. 2014

PLoS ONE

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Background De novo transcriptome sequencing is a robust method of predicting miRNA target genes, especially for organisms without reference genomes. Differentially expressed miRNAs had been identified previously in kidney samples collected from susceptible and resistant grass carp (Ctenopharyngodon idella) affected by Aeromonas hydrophila. Target identification for these differentially expressed miRNAs poses a major challenge in this non-model organism.ResultsTwo cDNA libraries constructed from mRNAs of susceptible and resistant C. idella were sequenced by Illumina Hiseq 2000 technology. A total of more than 100 million reads were generated and de novo assembled into 199,593 transcripts which were further extensively annotated by comparing their sequences to different protein databases. Biochemical pathways were predicted from these transcript sequences. A BLASTx analysis against a non-redundant protein database revealed that 61,373 unigenes coded for 28,311 annotated proteins. Two cDNA libraries from susceptible and resistant samples showed that 721 unigenes were expressed at significantly different levels; 475 were significantly up-regulated and 246 were significantly down-regulated in the SG samples compared to the RG samples. The computational prediction of miRNA targets from these differentially expressed genes identified 188 unigenes as the targets of 5 conserved and 4 putative novel miRNA families.ConclusionThis study demonstrates the feasibility of identifying miRNA targets by transcriptome analysis. The transcriptome assembly data represent a substantial increase in the genomic resources available for C. idella and will provide insights into the gene expression profile analysis and the miRNA function annotations in further studies.

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Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection

Cited by 21 publications

References 61 publications

The effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream ( Megalobrama amblycephala )

The effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream ( Megalobrama amblycephala )

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

De novo Assembly of the Grass Carp Ctenopharyngodon idella Transcriptome to Identify miRNA Targets Associated with Motile Aeromonad Septicemia

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