Differences of Rhizopus oryzae strains in organic acid synthesis and fatty acid composition

Oda, Yuji; Yajima, Yuka; Kinoshita, Mikio; Ohnishi, Masao

doi:10.1016/s0740-0020(02)00131-4

Cited by 51 publications

(22 citation statements)

References 23 publications

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“…9) These two distinct sequence types coincided with physiological characteristics such as organic acid production and lipid composition of plasma membrane. 21) Moreover, Saito et al 22) analyzed the structure of ldh (lactate dehydrogenase) genes from both ITS types and found that they are distinct from each other. Type II strains, viz., fumaric acid producers, lacked the ldhA gene, which is responsible for lactic acid production in type I strains, viz., lactic acid producers.…”

Section: Discussionmentioning

confidence: 99%

The Molecular Phylogeny of the GenusRhizopusBased on rDNA Sequences

Abe

Oda

Asano

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

The Molecular Phylogeny of the GenusRhizopusBased on rDNA Sequences

Abe

Oda

Asano

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…For the current fungi R. oryzae AS 3.254, 12.0 g/l exogenous NaCl (total salt concentration in the medium, termed as NaCl, was 13.2 g/ l) may be above the threshold level and lead to the great composition change of fatty acids in the membrane of R. oryzae AS 3.254, and further restrains its lactic acid production. Also, compared with R. oryzae IFO 4707 reported in the literature, R. oryzae AS 3.254 was more sensitive to NaCl whereas the former had no virtual change in fatty acid composition in 20.0 g/l NaCl medium [20]. Samples with fishmeal wastewater were diluted ten times as nitrogen source before 30.0 g/l glucose was added and no other nutrient was supplemented.…”

Section: Effect Of Nacl Concentrationmentioning

confidence: 99%

Biotechnological production of lactic acid integrated with fishmeal wastewater treatment by Rhizopus oryzae

Huang

Dong

Chen

et al. 2007

Bioprocess Biosyst Eng

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Fishmeal wastewater, a seafood processing waste, was utilized for production of lactic acid and fungal biomass by Rhizopus oryzae AS 3.254 with the addition of sugars. The 30 g/l exogenous glucose in fishmeal wastewater was superior to starch in view of productivities of lactic acid and fungal biomass, and COD reduction. Fishmeal wastewater can be a replacement for peptone which was the most suitable nitrogen source for lactic acid production among the tested organic or inorganic nitrogen sources. Exogenous NaCl (12 g/l) completely inhibited the production of lactic acid and fungal growth. In the medium of COD 5,000 mg/l fishmeal wastewater with the addition of 30 g/l glucose, the maximum productivity of lactic acid was 0.723 g/l h corresponding to productivity of fungal biomass 0.0925 g/l h, COD reduction 84.9% and total nitrogen removal 50.3% at a fermentation time of 30 h.

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“…The mycelium was taken from the surface of the agar and inoculated in 50 ml of a medium containing 50 g/l glucose, 6.7 g/l yeast nitrogen base without amino acids (Difco), 5.0 g/l casamino acids (Difco), and 25 g/l calcium carbonate. 15) After cultivation for 7 d at 25…”

Section: Methodsmentioning

confidence: 99%

“…Organic acids in the culture fluid were determined by high-performance liquid chromatography. 15) DNA analysis. Genomic DNA was isolated as described previously.…”

Section: Methodsmentioning

confidence: 99%