Different Biological Characteristics of Wild-Type Porcine Reproductive and Respiratory Syndrome Viruses and Vaccine Viruses and Identification of the Corresponding Genetic Determinants

Kim, Won-Il; Kim, Jaejo; Cha, Sang‐Ho; Yoon, Kyoung‐Jin

doi:10.1128/jcm.01927-07

Cited by 35 publications

(33 citation statements)

References 31 publications

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“…The supernatant was passed through a nonpyrogenic syringe filter with 0.2-µm pores (Pall Corporation, USA). The resulting suspensions were used as inocula to determine the viral titer using a plaque assay [14]. Briefly, each suspension was serially diluted 10-fold in alpha-MEM (Welgene, Korea) containing 2% (v/v) antibiotic-antimycotic solution (Lonza, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

Jung

Lee

2013

J Vet Sci

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Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model, dietary supplementation with GB enhanced concanavalin A (ConA)-induced lymphocyte proliferation and increased the percentage of CD3+CD8+ T lymphocytes. In pigs experimentally infected with PRRSV, viral titers in lungs and lymphoid tissues from the GB-fed group were significantly decreased compared to those of the control group 12 days post-infection. Corresponding histopathological analyses demonstrated that GB-fed pigs displayed less severe pathological changes associated with PRRSV infection compared to the control group, indicating that GB promotes PRRSV clearance. These antiviral effects in pigs may be related to the ability of GB to increase CD3+CD8+ T lymphocyte production observed in the mice. Hence, this mineral may be an effective feed supplement for increasing immune activity and preventing disease.

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Section: Methodsmentioning

confidence: 99%

Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

Jung

Lee

2013

J Vet Sci

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“…Genes of some wild-type viruses such as rotaviruses, [22][23][24][25] poliovirus, [26][27][28] varicella zoster virus, 29 porcine reproductive and respiratory syndrome virus 30 and transmissible gastroenteritis virus 31 and their attenuated forms derived by sequential passages in cell culture have been characterized to determine the molecular basis of attenuation. In this study, we isolated a G1P [8] human rotavirus strain CDC-9 from a child who had a mixed rotavirus G1P [8] and G2P [4] infection.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of human rotavirus vaccine strain CDC-9 during sequential passages in vero cells

Esona

Foytich²,

Wang³

et al. 2010

Human Vaccines

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“…In this study, three PRRSV strains, VR2332, MN184, and JA142 (ATP vaccine strain), which are phylogenetically highly divergent from each other (29,30), were used in the NA assay, and MN184 strain was also used for the infected pig groups. Two modified live PRRS virus vaccines, Ingelvac PRRS-ATP (Boehringer Ingelheim) and Ingelvac PRRS-MLV (Boehringer Ingelheim), derived from a parental strain (JA142) and a North American prototype strain (VR2332), respectively, were used in pigs.…”

Section: Methodsmentioning

confidence: 99%

“…These samples were pooled on each day of collection (22). (ii) Oral fluid and serum samples were collected from 24 boars vaccinated with PRRS-MLV and 24 boars infected with a type 2 PRRSV (MN184 strain) (30). The samples that were collected at 0 and 21 d.p.v.…”

Section: Methodsmentioning

confidence: 99%

Development and Validation of an Assay To Detect Porcine Reproductive and Respiratory Syndrome Virus-Specific Neutralizing Antibody Titers in Pig Oral Fluid Samples

Ouyang

Binjawadagi

Kittawornrat

et al. 2013

Clin Vaccine Immunol

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Porcine reproductive and respiratory syndrome virus (PRRSV)-specific neutralizing antibodies (NA) are important for clearing the virus. Pen-based pig oral fluid samples for disease surveillance are gaining in importance due to the ease of collection and low cost. The aim of this study was to develop a PRRSV-specific NA assay to determine NA titers in pig oral fluid samples. At first, we standardized the PRRSV NA assay using pen-based pig oral fluid samples collected over a period of 3 months from a herd of swine that received a PRRSV modified live vaccine (PRRS-MLV), and we also used oral fluid and serum samples collected from individual boars that were vaccinated with PRRS-MLV or infected with a virulent PRRSV strain. Our results suggest that a PRRSV NA titer of >8 in oral fluid samples is virus specific and can be detected beginning at 28 days after vaccination or infection. To validate the assay, we used 104 pen-based pig oral fluid and five representative serum samples from each pen of unknown history, as well as 100 serum samples from repeatedly vaccinated sows and oral fluid samples of their respective litters belonging to four different swine-breeding farms. Our results demonstrated that PRRSV NA titers in oral fluid samples are correlated with serum sample titers, and maternally derived PRRSV-specific NA titers could be detected in litters at the time of weaning. In conclusion, we have standardized and validated the pig oral fluid-based PRRSV NA assay, which has 94.3% specificity and 90.5% repeatability. The assay can be used to monitor herd immunity against PRRSV in vaccinated and infected herds of swine.

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Different Biological Characteristics of Wild-Type Porcine Reproductive and Respiratory Syndrome Viruses and Vaccine Viruses and Identification of the Corresponding Genetic Determinants

Cited by 35 publications

References 31 publications

Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

Molecular characterization of human rotavirus vaccine strain CDC-9 during sequential passages in vero cells

Development and Validation of an Assay To Detect Porcine Reproductive and Respiratory Syndrome Virus-Specific Neutralizing Antibody Titers in Pig Oral Fluid Samples

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