Different collection tubes in cardiac biomarkers detection

Abstract

The role played by different collection tubes is one of the more frequently forgotten parameters, which influences the quality of laboratory results.We aimed to determine whether there is a significant difference in assay of myoglobin, cardiac troponin I, and CK-MB depending on the presence of anticoagulant and gel separator in the collecting tubes.Blood samples were collected in 40 consecutive patients admitted for acute coronary syndrome. The samples were taken at the same moment in three different tubes (tubes without anticoagulant and with gel separator, heparin tubes with gel separator and heparin tubes without gelseparator;Venosafe, Terumo Europe, Leuven Belgium). Myoglobin, CK-MB and cardiac troponin I were measured with a double side (sandwich) chemiluminescent immuenzymatic assay (Access Myoglobin, Access CK-MB, Access AccuTnI).Cardiac troponin I was not influenced by the type of collection tube used. On the contrary, myoglobin and CK-MB showed a statistical difference depending on the test tube. In particular, there was a significant difference between tubes without anticoagulant and with gel separator and tubes with heparin and without gel.

Interferences from blood collection tube components on clinical chemistry assays

“…Organochlorine, polychlorinated biphenyl, and progesterone levels may also be significantly reduced (47). A small but statistically significant difference in myoglobin and CK-MB levels has been reported between tubes with and without separator gels (5). Interestingly, newer separator gels (e.g.…”

“…Glass evacuated tubes containing anticoagulants were commonly used from the 1950s to the 1990s ( 4 ). Presently, plastic has replaced glass, and polymer gels and clot activators are common additives ( 5 ). Despite their similarity, evacuated tubes supplied by different manufacturers vary in the materials and additives used, which can potentially affect test performance ( 6 ).…”

A dried blood spots technique based LC–MS/MS method for the analysis of posaconazole in human whole blood samples

“…The current sampling process is onerous in terms of time given that the physicians require rapid data turnaround in order to make relevant clinical decisions regarding very ill patients. It is important to note that collection methods that utilize separator gel tubes to harvest serum have the potential to underestimate exposure because of adsorption to the polymeric gel [46][47][48][49][50].…”

Analytical quality assessment and method comparison of two immunoassays for the measurement of serum cardiac Troponin I in dogs and cats

“…Whole blood samples of 2‐3 mL, obtained by jugular or cephalic venipuncture, were submitted in plain or gel separator tubes (various manufacturers) to the Diagnostic Laboratories. While different blood tubes have been shown to affect the concentration of some biochemical analytes, tube type did not affect cTnI concentrations in one study, and the kit inserts did not specify that separator tubes should not be used . After clot retraction, samples were centrifuged at 1751 g for 5 minutes.…”