Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens

Zhang, J. L.; Xie, Qiang; Ji, Junfu; Yang, Wen‐Chin; Wu, Yinbao; Li, C.; Y., J.; Bi, Yanlan

doi:10.3382/ps.2012-02339

Cited by 87 publications

(56 citation statements)

References 20 publications

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“…did not result in an increase in FCR (Ginzberg et al, 2000;Bozkurt et al, 2012). This variation may be attributed to the age or strain (or both) of birds used in the experiment and also the type of seaweed used and inclu- sion rates, as well as the differences in the experimental conditions such as temperature and humidity (Geier et al, 2009;zhang et al, 2012). The increase in egg and yolk weight with 1% level of seaweeds (1% CC and SG) might be attributed to higher yolk protein synthesis and an increase in water and mineral availability (Novak et al, 2004) and greater retention of feed in the gastrointestinal tract and thus a better ileal digestibility of the feed (Piray et al, 2007).…”

Section: Discussionmentioning

confidence: 90%

Feed supplementation with red seaweeds, Chondrus crispus and Sarcodiotheca gaudichaudii, affects performance, egg quality, and gut microbiota of layer hens

et al. 2014

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The aim of this study was to evaluate the effect of the inclusion of red seaweed supplementation to standard poultry diets on production performance, egg quality, intestinal histology, and cecal short-chain fatty acids in Lohmann Brown Classic laying hens. A total of 160 birds were randomly assigned to 8 treatment groups. Control hens were fed a basal layer diet; positive control hens were fed a diet containing 2% inulin; and 6 treatment groups were fed a diet containing one of the following; 0.5, 1, or 2% Chondrus crispus (CC0.5, CC1, and CC2, respectively) and one of the same 3 levels of Sarcodiotheca gaudichaudii (SG0.5, SG1, and SG2, respectively). Dietary supplementation had no significant effect on the feed intake, BW, egg production, fecal moisture content, and blood serum profile of the birds. The feed conversion ratio per gram of egg was significantly more efficient (P = 0.001) for CC2 and SG2 treatments. Moreover, SG1 supplementation increased egg yolk weight (P = 0.0035) and birds with CC1 supplementation had higher egg weight (P = 0.0006). The SG2 and CC2 groups had greater (P < 0.05) villus height and villus surface area compared with the control birds. Seaweed supplementation increased the abundance of beneficial bacteria [e.g., Bifidobacterium longum (4- to 14-fold), Streptococcus salivarius (4- to 15-fold)] and importantly reduced the prevalence of Clostridium perfringens in the gut of the chicken. Additionally, the concentrations of short-chain fatty acids, including acetic acid, propionic acid, n-butyric acid, and i-butyric acid, were significantly higher (P < 0.05) in CC and SG treatments than in the control. In conclusion, dietary supplementation using red seaweed inclusions can act as a potential prebiotic to improve performance, egg quality, and overall gut health in layer hens.

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Section: Discussionmentioning

confidence: 90%

Feed supplementation with red seaweeds, Chondrus crispus and Sarcodiotheca gaudichaudii, affects performance, egg quality, and gut microbiota of layer hens

et al. 2014

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“…However, a decrease in the relative abundance of Bacilli in the probiotic-fed groups compared to the control, which was observed in the developer and layer phases, may suggest a feedback of lessened potential pathogens after its supplementation (72,76), possibly creating a conducive environment in modifying the structure of the cecal microbiome. On the other hand, probiotics containing dead organisms have also been shown to express beneficial effects (77,78), such as by inducing specific immune responses based on the ability of immune cells to recognize individual bacterial cell structural components (79). These theories may suggest possible indirect attributes of the probiotic strain used in the present study, which were evidenced through the improved AR of most components, in the developer phase for the probiotic supplemented group, particularly with the LSSB and MSSB, despite a decreased relative abundance of Bacilli in the former dose of supplementation, compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

Bacillus subtilis Strain DSM 29784 Modulates the Cecal Microbiome, Concentration of Short-Chain Fatty Acids, and Apparent Retention of Dietary Components in Shaver White Chickens during Grower, Developer, and Laying Phases

Neijat

Habtewold

Shirley³

et al. 2019

Appl Environ Microbiol

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This study investigated the efficacy of a single strain of Bacillus subtilis (SSB) in modulating the composition of cecal microbiota and its link to the concentration of short-chain fatty acids (SCFA) and apparent retention (AR) of components. A total of 720, 4-week-old Shaver White chicks were allotted to control (CON), 1.1Eϩ08 (low, LSSB), 2.2Eϩ08 (medium, MSSB), or 1.1Eϩ09 (high, HSSB) CFU/kg of diet groups. At grower (10-week), developer (16-week), and laying (28-week) phases, excreta and cecal digesta samples were taken for AR, microbial, and SCFA analyses. Microbial analysis involved high-throughput sequencing of the V3-V4 hypervariable regions of 16S rRNA gene. Bacterial diversity decreased (P Ͻ 0.05) at the developer phase as the SSB dose increased; however, a distinct clustering pattern (P Ͻ 0.05) of bacterial community was noted. Bacteroides and Faecalibacterium were differentially enriched in the developer for SSB-fed compared to CON-fed birds. Although no differences in microbial diversity were detected in grower and layer phases, different species of Clostridium (XVIII, XIVa, IV, and XIVb)-major butyrate producers-were identified in all phases, with stronger effect sizes for SSB-fed compared to CON-fed birds. Isobutyric acid was elevated in dose response (P ϭ 0.034) in layer phase. In addition, the relative abundances of Alistipes, Lactobacillus, and Bifidobacterium were positively correlated (P Ͻ 0.05), with AR of most components for SSB-fed birds in the pullet phase. The results suggested that supplementing chickens' diet with B. subtilis DSM 29784 may selectively enrich beneficial bacterial communities, which in turn are critical in promoting the growth and performance of hens. IMPORTANCE In egg-laying chickens, the trend in the move away from the cage to alternative housing systems and restriction in antimicrobial use requires alternative approaches to maintain health and prevent diseases. There is increased research and commercial interest toward alternative gut health solutions while improving the performance and product safety in poultry production systems. One such approach, in recognition of the importance of the gut microbial community, is the use of microbes as feed supplements (such as probiotics). Unlike meat-type chickens, studies assessing the efficacy of such microbial supplements are limited for egg-laying chickens. Thus, by conducting a comprehensive assessment of the hen microbiota in response to various levels of B. subtilis DSM 29784 during the pullet phase (grower and developer) and the layer phase, the present study demonstrates the importance of direct-fed microbes in modulating gut microbiome, which may relate to improved performance efficiency in the pullet and layer phases.Citation Neijat M, Habtewold J, Shirley RB, Welsher A, Barton J, Thiery P, Kiarie E. 2019. Bacillus subtilis strain DSM 29784 modulates the cecal microbiome, concentration of shortchain fatty acids, and apparent retention of dietary components in Shaver White chickens during grower, developer, and laying ...

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“…The body immune status improved by regulating the intestine mucosal cytokines (Patterson & Burkholder, ). However, some studies found that dietary probiotics in diets showed little beneficial effects on its immune status, and this might be due to the different experimental design and the different time of collection (Mountzouris et al, ; Zhang, Xie, et al, ). As for the serum antibody levels of laying breeders, we found that supplementation with B. subtilis C‐3102 improved AIV‐Ab level in a linear manner, and there were no differences for NDV‐Ab level among all groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of Bacillus subtilis C‐3102 on production, hatching performance, egg quality, serum antioxidant capacity and immune response of laying breeders

Liu

Canyang

et al. 2018

Animal Physiology Nutrition

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To investigate the supplemental effects of Bacillus subtilis C‐3102 on the production, hatching performance, egg quality, serum antioxidant capacity and immune response of laying breeders, a total of 480 Xuefeng black‐bone (25‐week‐old) hens were randomly assigned into four treatment groups: Hens fed the basal diets with 0 (CON), 3.0 × 105 (BS‐1), 6.0 × 105 cfu/g (BS‐2) and 9.0 × 105 (BS‐3) cfu/g of B. subtilis C‐3102. As the B. subtilis C‐3102 level increased, egg weight (linear, p < 0.01; quadratic, p = 0.003), fertility (linear, p = 0.021; quadratic, p = 0.059), hatchability (linear, p = 0.038; quadratic, p = 0.119) and yolk colour (linear, p = 0.006; quadratic, p = 0.021) increased in a linear or quadratic manner. Yolk index increased quadratically (linear, p = 0.054; quadratic, p = 0.017), and eggshell thickness (linear, p = 0.036; quadratic, p = 0.128), the activity of GSH‐Px (linear, p = 0.024; quadratic, p = 0.078), the concentration of IgM (linear, p = 0.016; quadratic, p = 0.056) and the level of AIV‐Ab (linear, p = 0.034; quadratic, p = 0.103) in the serum increased linearly as dietary supplementation of B. subtilis C‐3102 increased. The results showed that dietary treatments did not affect egg production, feed conversion ratio, egg mass, hatchability of fertile eggs, eggshell‐breaking strength, egg‐shape index, yolk percentage, Haugh unit, T‐SOD, T‐AOC, MDA, IgA and IgG concentrations and the level of NDV‐Ab in the serum. In conclusion, dietary supplementation of 9.0 × 105 cfu/g B. subtilis C‐3102 in laying breeders diets may be a feasible means of effectively increasing egg weight, fertility and hatchability, and improving egg quality such as eggshell thickness, yolk index and yolk colour. Besides, B. subtilis C‐3102 can enhance the activity of GSH‐Px, the concentration of IgM and the level of AIV‐Ab in the serum.

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Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens

Cited by 87 publications

References 20 publications

Feed supplementation with red seaweeds, Chondrus crispus and Sarcodiotheca gaudichaudii, affects performance, egg quality, and gut microbiota of layer hens

Feed supplementation with red seaweeds, Chondrus crispus and Sarcodiotheca gaudichaudii, affects performance, egg quality, and gut microbiota of layer hens

Bacillus subtilis Strain DSM 29784 Modulates the Cecal Microbiome, Concentration of Short-Chain Fatty Acids, and Apparent Retention of Dietary Components in Shaver White Chickens during Grower, Developer, and Laying Phases

Effects of Bacillus subtilis C‐3102 on production, hatching performance, egg quality, serum antioxidant capacity and immune response of laying breeders

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