Different DNA replication behavior of a tandem duplication in Drosophila melanogaster

Kalisch, W.-E.; Hägele, Klaus

doi:10.1007/bf00291021

Cited by 17 publications

(1 citation statement)

References 20 publications

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“…This method allowed researchers to draw quantitative conclusions about the intensity of label inclusion after silver grain counting. Various authors have identified up to seven categories of labeling patterns and arranged them chronologically (Rodman 1968 ; Kalisch and Hägele 1973 ; Roy and Lakhotia 1979 , 1981 ; Achary et al 1981 ; Mishra and Lakhotia 1982 ). The labels have been detected in decondensed areas (called interbands in the cited papers, corresponding to our INTs) in three early stages representing low, medium, and heavy interband patterns.…”

Section: Discussionmentioning

confidence: 99%

Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes

et al. 2022

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Studying the probability distribution of replication initiation along a chromosome is a huge challenge. Drosophila polytene chromosomes in combination with super-resolution microscopy provide a unique opportunity for analyzing the probabilistic nature of replication initiation at the ultrastructural level. Here, we developed a method for synchronizing S-phase induction among salivary gland cells. An analysis of the replication label distribution in the first minutes of S phase and in the following hours after the induction revealed the dynamics of replication initiation. Spatial super-resolution structured illumination microscopy allowed identifying multiple discrete replication signals and to investigate the behavior of replication signals in the first minutes of the S phase at the ultrastructural level. We identified replication initiation zones where initiation occurs stochastically. These zones differ significantly in the probability of replication initiation per time unit. There are zones in which initiation occurs on most strands of the polytene chromosome in a few minutes. In other zones, the initiation on all strands takes several hours. Compact bands are free of replication initiation events, and the replication runs from outer edges to the middle, where band shapes may alter.

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Section: Discussionmentioning

confidence: 99%

Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes

et al. 2022

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Comparative Aspects of Chromosome Replication in Drosophila and Mammals

Raman¹,

Lakhotia²

1990

Trends in Chromosome Research

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Patterns of polytene-chromosome replication in Simulium ornatipes (Diptera: Simuliidae)

Bedo

1982

Genetica

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Double labelling of Simulium ornatipes polytene chromosomes with H3-and CiJ-thymidine shows that chromosome synthesis follows three distinct phases viz. a short phase of initiation in puffs and interbands spreading to more condensed regions; a long continuous labelling phase, then a discontinuously labelled end phase as bands complete their replication in temporal sequence. Analysis of H3 labelling patterns indicates that while heterochromatic bands replicate there is no clear correlation between heterochromatic or C-banding regions and band replication time. The major characteristic governing band replication time appears to be band size and density. However, in some bands this relationship is modified, perhaps it is suggested, by DNA organisation influencing the efficiency of replicons. The existence of great variability in homologous band replication times, even within a chromosome pair, indicates that the control of band replication is highly autonomous. It is suggested that polymorphisms at the molecular level determine this variation. Replication time of active nucleolar organisers is very long in contrast to the short replication of condensed inactive organisers. This may reflect differential polytenisation of ribosomal DNA as a result of a developmental polymorphism, or the amplification of ribosomal DNA by active nucleolar organisers.

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Different DNA replication behavior of a tandem duplication in Drosophila melanogaster

Cited by 17 publications

References 20 publications

Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes

Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes

Comparative Aspects of Chromosome Replication in Drosophila and Mammals

Patterns of polytene-chromosome replication in Simulium ornatipes (Diptera: Simuliidae)

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