Different domains of glycoprotein 96 influence HPV16 E7 DNA vaccine potency via electroporation mediated delivery in tumor mice model

How can Improve DNA Vaccine Modalities as a Therapeutic Approach against HIV Infections?

“…Therefore, lower doses of DNA would potentially facilitate clearance of plasmids from injection site quickly resulting in lower risks of toxicity [18]. To enhance immunological priming by DNA vaccine, physical delivery by electroporation (EP) has further been studied [18,19]. Electroporation utilizes a small electric field across the site of injection (the muscle or skin) causing temporary membrane instability and also an electric gradient, which increases the cellular uptake of DNA [13,19,20].…”

“…To enhance immunological priming by DNA vaccine, physical delivery by electroporation (EP) has further been studied [18,19]. Electroporation utilizes a small electric field across the site of injection (the muscle or skin) causing temporary membrane instability and also an electric gradient, which increases the cellular uptake of DNA [13,19,20]. EP could overcome the failure of low-volume inoculation to induce immunity in mice, and the combination of DNA priming with DNA/EP at boosting was remarkably effective.…”

Electroporation – Advantages and Drawbacks for Delivery of Drug, Gene and Vaccine

“…Electroporation has been used to enhance immune responses using DNA vaccines directed against infectious diseases such as influenza, HIV, hepatitis C, malaria, anthrax or to treat or prevent the development of tumors including breast cancer, prostate cancer and melanoma [Daemi et al, 2012;Best et al, 2009]. The studies have shown that in vivo EP mediated vaccination is a safe and effective modality for the treatment of prostate cancer and has potential to be used as a neo-adjuvant or adjuvant therapy [Ahmad et al, 2010].…”

“…), currently, skin EP is used as an attractive and less invasive option that is able to induce robust adaptive immune responses. To date, studies of DNA EP in skin have mainly focused on antigen expression, antigen specific humoral immunity, induction of IFN-γ-producing T cells and protective efficacy to infection [Daemi et al, 2012;Brave et al, 2011]. Plasmid DNA vaccination using skin electroporation (EP) is a promising method able to elicit robust humoral and CD8+T-cell immune responses while limiting invasiveness of delivery [Brave et al, 2011].…”

“…The voltages generally used for electroporation in animals range from 100 to 1200 V/cm. The investigators have shown that low-voltage electroporation can induce immunity and protect mice effectively [Daemi et al, 2012;Zhou et al, 2008]. In addition, intradermal DNA electroporation is one of the most efficient non-viral methods for the delivery of gene into the skin .…”