Different effects of hydrogen-rich water intake and hydrogen gas inhalation on gut microbiome and plasma metabolites of rats in health status

Xie, Fei; Jiang, Xue; Yi, Yang; Liu, Zijia; Ma, Chen; He, Jin; Xun, Zhi-ming; Wang, Meng; Liu, Mengyu; Adzavon, Yao Mawulikplimi; Zhao, Pengxiang; Ma, Xuemei

doi:10.1038/s41598-022-11091-1

Cited by 19 publications

(10 citation statements)

References 54 publications

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“…Specifically, both MGH and CMG treatments significantly enhanced phenylalanine and tyrosine metabolism, which are known to be closely linked to oxidative stress response ( Ipson et al, 2019 ). Our findings are consistent with a previous study that reported significant alternations in the fecal microbiota of rats following hydrogen gas treatment, leading to increased phenylalanine, tyrosine and tryptophan biosynthesis ( Xie et al, 2022 ). The gut microbiome plays a role in regulating intestinal functions through its metabolites, including hydrogen gas and SCFAs.…”

Section: Discussionsupporting

confidence: 93%

In vitro fermentation properties of magnesium hydride and related modulation effects on broiler cecal microbiome and metabolome

Zhu

Yang

et al. 2023

Front. Microbiol.

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Magnesium hydride (MGH), a highly promising hydrogen-producing substance/additive for hydrogen production through its hydrolysis reaction, has the potential to enhance broiler production. However, before incorporating MGH as a hydrogen-producing additive in broiler feed, it is crucial to fully understand its impact on microbiota and metabolites. In vitro fermentation models provide a fast, reproducible, and direct assessment tool for microbiota metabolism and composition. This study aims to investigate the effects of MGH and coated-magnesium hydride (CMG) on fermentation characteristics, as well as the microbiota and metabolome in the culture of in vitro fermentation using cecal inocula from broilers. After 48 h of incubation, it was observed that the presence of MGH had a significant impact on various factors. Specifically, the content of N-NH3 decreased, while the total hydrogen gas and total SCFAs increased. Furthermore, the presence of MGH promoted the abundance of SCFA-producing bacteria such as Ruminococcus, Blautia, Coprobacillus, and Dysgonomonas. On the other hand, the presence of CMG led to an increase in the concentration of lactic acid, acetic acid, and valeric acid. Additionally, CMG affected the diversity of microbiota in the culture, resulting in an enrichment of the relative abundance of Firmicutes, as well as genera of Lactobacillus, Coprococcus, and Eubacterium. Conversely, the relative abundance of the phylum Proteobacteria and pathogenic bacteria Shigella decreased. Metabolome analysis revealed that MGH and CMG treatment caused significant changes in 21 co-regulated metabolites, primarily associated with lipid, amino acid, benzenoids, and organooxygen compounds. Importantly, joint correlation analysis revealed that MGH or CMG treatments had a direct impact on the microbiota, which in turn indirectly influenced metabolites in the culture. In summary, the results of this study suggested that both MGH and coated-MGH have similar yet distinct positive effects on the microbiota and metabolites of the broiler cecal in an in vitro fermentation model.

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Section: Discussionsupporting

confidence: 93%

In vitro fermentation properties of magnesium hydride and related modulation effects on broiler cecal microbiome and metabolome

Zhu

Yang

et al. 2023

Front. Microbiol.

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“…A wide diversity of gut microbes metabolize H 2 ( 47 ), such that H 2 from C. minuta may boost their growth. Indeed, hydrogen administration to the gut microbiome in drinking water has been shown to alter gut microbial diversity ( 48 , 49 ). Mice receiving hydrogen-water have also been shown to produce greater levels of SCFAs in the cecum ( 50 ), which is consistent with our observations of greater cecal SCFAs in Live-CM treated female mice.…”

Section: Discussionmentioning

confidence: 99%

The keystone gut species Christensenella minuta boosts gut microbial biomass and voluntary physical activity in mice

Akbuğa-Schön,

Suzuki,

Jakob

et al. 2024

mBio

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The gut bacteria of the family Christensenellaceae are consistently associated with metabolic health, but their role in promoting host health is not fully understood. Here, we explored the effect of Christensenella minuta amendment on voluntary physical activity and the gut microbiome. We inoculated male and female germ-free mice with an obese human donor microbiota together with live or heat-killed C. minuta for 28 days and measured physical activity in respirometry cages. Compared to heat-killed, the live- C. minuta treatment resulted in reduced feed efficiency and higher levels of physical activity, with significantly greater distance traveled for males and higher levels of small movements and resting metabolic rate in females. Sex-specific effects of C. minuta treatment may be in part attributable to different housing conditions for males and females. Amendment with live C. minuta boosted gut microbial biomass in both sexes, immobilizing dietary carbon in the microbiome, and mice with high levels of C. minuta lose more energy in stool. Live C. minuta also reduced within and between-host gut microbial diversity. Overall, our results showed that C. minuta acts as a keystone species: despite low relative abundance, it has a large impact on its ecosystem, from the microbiome to host energy homeostasis. IMPORTANCE The composition of the human gut microbiome is associated with human health. Within the human gut microbiome, the relative abundance of the bacterial family Christensenellaceae has been shown to correlate with metabolic health and a lean body type. The mechanisms underpinning this effect remain unclear. Here, we show that live C. minuta influences host physical activity and metabolic energy expenditure, accompanied by changes in murine metabolism and the gut microbial community in a sex-dependent manner in comparison to heat-killed C. minuta . Importantly, live C. minuta boosts the biomass of the microbiome in the gut, and a higher level of C. minuta is associated with greater loss of energy in stool. These observations indicate that modulation of activity levels and changes to the microbiome are ways in which the Christensenellaceae can influence host energy homeostasis and health.

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“…As redox modulation is known to play a crucial role in de novo lipogenesis [ 27 ], the anti-oxidative activity of coral hydrate might be related to the measured decreases in serum triglycerides. Alternatively, changes in the gut microbiome might mediate lipid metabolism [ 28 ] to produce the observed effects, since consumption of hydrogenated water alters gut microbiome and plasma metabolites in rats [ 29 ]. Thus, coral hydrate might not only directly regulate lipid metabolism in the liver, but it may also affect lipid metabolism indirectly through changes in the gut microbiome.…”

Section: Discussionmentioning

confidence: 99%

Coral Hydrate, a Novel Antioxidant, Improves Alcohol Intoxication in Mice

Chao

et al. 2022

Antioxidants

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Alcohol-drinking culture may cause individuals to periodically experience unpleasant hangovers. In addition, ethanol catabolism stimulates the production of free radicals that may cause liver injury and further lead to the development of chronic alcoholic fatty liver disease. Although a number of studies have suggested that hydrogenated water may be consumed to act as free radical scavenger, its instability limits its application. In this study, we used coral hydrate (i.e., hydrogenated coral materials) as a more stable hydrogen source and evaluated its effects in a murine model of alcohol intoxication. In solution, coral hydrate exhibited much more stable redox potential than did hydrogenated water. Furthermore, administration of coral hydrate by oral gavage significantly prolonged the time to fall asleep and decreased the total sleep time in mice that received intraperitoneal injection of ethanol. The mice receiving coral hydrate also had lower plasma ethanol and acetaldehyde levels than controls. In line with this observation, hepatic expression of alcohol dehydrogenase, acetaldehyde dehydrogenase, catalase and glutathione peroxidase were all significantly increased by the treatment. Meanwhile, alcohol-induced upregulation of pro-inflammatory factors was attenuated by the administration of coral hydrate. Taken together, our data suggest that coral hydrate might be an effective novel treatment for alcohol intoxication.

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Different effects of hydrogen-rich water intake and hydrogen gas inhalation on gut microbiome and plasma metabolites of rats in health status

Cited by 19 publications

References 54 publications

In vitro fermentation properties of magnesium hydride and related modulation effects on broiler cecal microbiome and metabolome

In vitro fermentation properties of magnesium hydride and related modulation effects on broiler cecal microbiome and metabolome

The keystone gut species Christensenella minuta boosts gut microbial biomass and voluntary physical activity in mice

Coral Hydrate, a Novel Antioxidant, Improves Alcohol Intoxication in Mice

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