Different Effects of Imidapril and Enalapril on Aminopeptidase P Activity in the Mouse Trachea

Sakamoto, Kouichi; Sugimoto, Koh‐ichi; Sudoh, Toshiaki; Fujimura, Akio

doi:10.1291/hypres.28.243

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…However, just molecular size cannot explain the differences in inhibition. Comparatively, the angiotensin-converting enzyme (ACE) inhibition, another member of the aminopeptidase family with the similar catalytic domain and zinc ion as a cofactor, shows an inverse correlation between molecular volume and activity [29,30,31]. As observed by the SiteMap analyses (Figure 3E), the closed conformation of the pAMP active site have small contributions of hydrophobic residues and, therefore, the insertion of a methyl group (increasing hydrophobicity), could be deleterious, when not addressing proper pockets.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

et al. 2019

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Aminopeptidase M (AMP) inhibition is of interest for several diseases, such as highly vascularized cancer types. AMP can be inhibited by linear pentapeptides isolated from Microcystis aeruginosa LTPNA08 (MG7XX). Porcine AMP inhibition—a model for human AMP—activity was spectrophotometrically measured by the formation of p-nitroanilide from L-leucine-p-nitroanilide substrate by AMP. AMP inhibition by MG770 exhibited comparable inhibition levels to amastatin (IC50 values: 1.20 ± 0.1 μM and 0.98 ± 0.1 μM, respectively), while MG756 was slightly less potent (with IC50 values of 3.26 ± 0.5 μM). Molecular modelling suggests a potential binding mode, based on the interaction with the Zn2+ cofactor, where MG770′s extra methyl group contributes to the disturbance of the Zn2+ cofactor complex and highlights the importance of hydrophobicity for the site.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

et al. 2019

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“…Oxopyrrolidine molecules with a proline backbone have been considered for a wide range of applications such as ACE inhibitors, antibacterial, antitumor properties and applications in asymmetric catalysis [1][2][3]. The asymmetric synthesis of pyrrolidine derivatives for both biological and catalytic purposes has received signi cant attention.…”

Section: Discussionmentioning

confidence: 99%

Crystal structure of 2-ethyl-1-tert-butyl-2-((4-fluorophenyl)(tert-butoxycarbonylamino)methyl)-3-oxo-pyrrolidine-1,2-dicarboxylate, C₂₄H₃₃FN₂O₇

Makhathini

Samipillai

Govender

et al. 2016

Zeitschrift Für Kristallographie - New Crystal Structures

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“…All inhibitors and antagonists were given by intraperitoneal injection. Ang-II (Sigma Aldrich, 1.44 mg per kg) and enalapril (Sigma Aldrich, 1 mg per kg) were dissolved in PBS 60 . PBS dissolved L-NAME (Sigma Aldrich, 10 mg per kg) was injected and PB was drawn at 10 minutes post-administration 61 .…”

Section: Methodsmentioning

confidence: 99%

Vasculopathy-associated hyperangiotensinemia mobilizes haematopoietic stem cells/progenitors through endothelial AT2R and cytoskeletal dysregulation

et al. 2015

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Patients in organ failure of vascular origin have increased circulating hematopoietic stem cells and progenitors (HSC/P). Plasma levels of angiotensin II (Ang-II), are commonly increased in vasculopathies. Hyperangiotensinemia results in activation of a very distinct Ang-II receptor set, Rho-family GTPase members, and actin in bone marrow endothelial cells (BMEC) and HSC/P, which results in decreased membrane integrin activation in both BMEC and HSC/P, and in HSC/P de-adhesion and mobilization. The Ang-II effect can be reversed pharmacologically and genetically by inhibiting Ang-II production or signaling through BMEC AT2R, HSCP AT1R/AT2R or HSC/P RhoA, but not by interfering with other vascular tone mediators. Hyperangiotensinemia and high counts of circulating HSC/P seen in sickle cell disease (SCD) as a result of vascular damage, is significantly decreased by Ang-II inhibitors. Our data define for the first time the role of Ang-II HSC/P traffic regulation and redefine the hematopoietic consequences of anti-angiotensin therapy in SCD.

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Different Effects of Imidapril and Enalapril on Aminopeptidase P Activity in the Mouse Trachea

Cited by 10 publications

References 17 publications

Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

Crystal structure of 2-ethyl-1-tert-butyl-2-((4-fluorophenyl)(tert-butoxycarbonylamino)methyl)-3-oxo-pyrrolidine-1,2-dicarboxylate, C₂₄H₃₃FN₂O₇

Vasculopathy-associated hyperangiotensinemia mobilizes haematopoietic stem cells/progenitors through endothelial AT2R and cytoskeletal dysregulation

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Different Effects of Imidapril and Enalapril on Aminopeptidase P Activity in the Mouse Trachea

Cited by 10 publications

References 17 publications

Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

Crystal structure of 2-ethyl-1-tert-butyl-2-((4-fluorophenyl)(tert-butoxycarbonylamino)methyl)-3-oxo-pyrrolidine-1,2-dicarboxylate, C24H33FN2O7

Vasculopathy-associated hyperangiotensinemia mobilizes haematopoietic stem cells/progenitors through endothelial AT2R and cytoskeletal dysregulation

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Crystal structure of 2-ethyl-1-tert-butyl-2-((4-fluorophenyl)(tert-butoxycarbonylamino)methyl)-3-oxo-pyrrolidine-1,2-dicarboxylate, C₂₄H₃₃FN₂O₇