2017
DOI: 10.1128/jvi.01379-16
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Different Expression of Interferon-Stimulated Genes in Response to HIV-1 Infection in Dendritic Cells Based on Their Maturation State

Abstract: Dendritic cells (DCs) are professional antigen-presenting cells whose functions are dependent on their degree of differentiation. In their immature state, DCs capture pathogens and migrate to the lymph nodes. During this process, DCs become resident mature cells specialized in antigen presentation. DCs are characterized by a highly limiting environment for human immunodeficiency virus type 1 (HIV-1) replication due to the expression of restriction factors such as SAMHD1 and APOBEC3G. However, uninfected DCs ca… Show more

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Cited by 6 publications
(4 citation statements)
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“…There are two possible reasons for this phenomenon: On the one hand, pDCs that are persistently stimulated by IFN-I express low levels of migration receptors and regulatory factors, such as CCR7, CD40, and CD86. These pDCs produce IFN-I since pathogenic nucleic acid traffics to the endosome ( 72 , 73 ), which induces apoptosis to these out-of-control cells mediated by the TNF-related apoptosis-inducing ligand (TRAIL) ( 74 ). On the other hand, IFN-I activates the non-canonical NF-κB signaling in pDCs.…”
Section: Effects Of Ifn-i On Immunocytes In Chronic Hiv-1 Infectionmentioning
confidence: 99%
“…There are two possible reasons for this phenomenon: On the one hand, pDCs that are persistently stimulated by IFN-I express low levels of migration receptors and regulatory factors, such as CCR7, CD40, and CD86. These pDCs produce IFN-I since pathogenic nucleic acid traffics to the endosome ( 72 , 73 ), which induces apoptosis to these out-of-control cells mediated by the TNF-related apoptosis-inducing ligand (TRAIL) ( 74 ). On the other hand, IFN-I activates the non-canonical NF-κB signaling in pDCs.…”
Section: Effects Of Ifn-i On Immunocytes In Chronic Hiv-1 Infectionmentioning
confidence: 99%
“…We evaluated the infection in human dendritic cells (DCs) and macrophages, because CAV1 is the major coat protein responsible for caveolae assembly and is highly expressed in these cell types [24]. Primary cells were generated and cultured as previously described [25].…”
Section: Cell Culturesmentioning
confidence: 99%
“…Briefly, HeLaeP4 cells, macrophages or DCs were seeded into 24-well plates and 100 ng of siRNAs and 3 mL of HiPerfect were used for each transfection. Immunoblotting, immunofluorescence, detection of HIV-1 core antigens by flow cytometry and the measurement of luciferase activity were carried out as previously described [25]. The antibodies employed for these experiments were a mouse anti-UBXN6 antibody (5C3-1, Abcam Inc.), a rabbit anti-Caveolin-1 antibody (N20, sc-894, Santa Cruz Biotechnology) and a FITCconjugated human anti-Gag/p24 (KC57, Beckman Coulter).…”
Section: Ubxn6 Knockdownmentioning
confidence: 99%
“…On the contrary, in the case of mDCs, productive HIV-1 infection decreased expression of interferon-stimulated genes CXCR3-binding chemokines, suggesting a diminished trans-infection of CD4 lymphocytes. Paradoxically, restrictive HIV-1 infection had the opposite effect on mDCs, increasing the aforementioned gene expression, which would result in lymphocyte attraction and enhanced trans-infection (161).…”
Section: Role Of Dendritic Cells In Hiv-1 Infectionmentioning
confidence: 99%