Different forms of administration of biotherapy 7dH in mice experimentally infected by Trypanosoma cruzi produce different effects

Ferraz, Fabiana Nabarro; Simoni, Geysa Karla; Nascimento, Anélio do; Melo, Carolina Sundin de; Aleixo, Denise Lessa; Gomes, Mônica Lúcia; Spack, Miguel; Araújo, Silvana Márques de

doi:10.1016/j.homp.2011.05.006

Cited by 27 publications

(32 citation statements)

References 24 publications

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“…This result is consistent with low levels of parasitemia and provides information about the benefit of the treatment scheme used, since survival in the murine model is directly related to parasitemia levels [27]. …”

Section: Resultssupporting

confidence: 73%

“…The other had negative PCR (Polymerase Chain Reaction) [24], understood as a protective action of the drug, since the treatment began at the time of the infection. Considering that the T. cruzi-Y strain shows 100% infectivity and mortality in Swiss mice [12,25-27] this result shows a patent benefit of the medication.…”

Section: Resultsmentioning

confidence: 99%

“…The different treatment schemes were based on the effect of medication linked to the immunological effect [3,18] and on the specific evolution of T. cruzi Y strain in Swiss mice [12,27]. Thus, BIOT PI and BIOT 4PI simulated a situation where the medication is offered when the immunological system is not in contact with the parasite, and a situation where the medication is offered when the immunological system is already in contact with the parasite, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, BIOT PI and BIOT 4PI simulated a situation where the medication is offered when the immunological system is not in contact with the parasite, and a situation where the medication is offered when the immunological system is already in contact with the parasite, respectively. In BIOT 4-5–6 and BIOT 7-8–9 , the medication was offered to animals just prior to the parasite peak and exactly coincident with the parasite peak, respectively [12,27]. …”

Section: Methodsmentioning

confidence: 99%

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Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi

et al. 2012

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BackgroundThere is no published information about the use of different protocols to administer a highly diluted medication.Evaluate the effect of different protocols for treatment with biotherapic T. cruzi 17 dH (BIOTTc17dH) on clinical/parasitological evolution of mice infected with T. cruzi-Y strain.MethodsA blind, randomized controlled trial was performed twice, using 60 28-day-old male Swiss mice infected with T. cruzi-Y strain, in five treatment groups: CI - treated with a 7% ethanol-water solution, diluted in water (10 μL/mL) ad libitum; BIOTPI - treated with BIOTTc17dH in water (10 μL/mL) ad libitum during a period that started on the day of infection; BIOT4DI - treated with BIOTTc17dH in water (10 μL/mL) ad libitum beginning on the 4th day of infection; BIOT4-5–6 - treated with BIOTTc17dH by gavage (0.2 mL/ animal/day) on the 4th, 5th and 6th days after infection; BIOT7-8–9 - treated with BIOTTc17dH by gavage (0.2 mL/ animal/day) on the 7th, 8th and 9th days after infection. We evaluated: parasitemia; total parasitemia (Ptotal); maximum peak of parasites; prepatent period (PPP) - time from infection to detection of the parasite in blood; patent period (PP) - period when the parasitemia can be detected in blood; clinical aspects; and mortality.ResultsParasitological parameters in the BIOTPI and mainly in the BIOT4PI group showed better evolution of the infection compared to the control group (CI), with lower Ptotal, lower maximum peak of parasites, higher PPP, lower PP and longer survival times. These animals showed stable body temperature and higher weight gain and water consumption, with more animals having normal-appearing fur for longer periods. In contrast, groups BIOT4-5–6 and BIOT7-8–9 showed worse evolution of the infection compared to the control group, considering both parasitological and clinical parameters. The correlation analysis combined with the other data from this study indicated that the prepatent period is the best parameter to evaluate the effect of a medication in this model.ConclusionsThe BIOT4DI group showed the best clinical and parasitological evolution, with lower parasitemia and a trend toward lower mortality and a longer survival period. The prepatent period was the best parameter to evaluate the effect of a medication in this model.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi

et al. 2012

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“…Previous studies have investigated the prophylactic and therapeutic effects of biotherapies in acute murine infection by this protozoan. These studies reported mainly parasitological, clinical, histopathological and hematological effects (Aleixo et al, 2012;Almeida et al, 2008;Ferraz et al, 2011Ferraz et al, , 2014aQueiroz et al, 2006;Sandri et al, 2015). However, only a few studies have directly investigated the immunomodulatory effects of biotherapies against T. cruzi infection (Almeida et al, 2008;Ferraz et al, 2014b;Queiroz et al, 2006;Sandri et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Modulation of IFN-γ, IL-4 and IL-17 Cytokines is Related to Parasitemia Control in Mice Infected by <i>Trypanosoma cruzi</i> and Treated with Biotherapy

Ferraz¹,

Veiga²,

Aleixo³

et al. 2015

OnLine Journal of Biological Sciences

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The study of biotherapies as an intervention in experimental models of infection is a possible means to understand the effects of these highly diluted medications. The present study evaluated the immunological and parasitological effects of biotherapies that were prepared from mouse serum that was uninfected (sarcode: BSNI 13cH group) and chronically infected with the Y strain of T. cruzi (nosode: BSI 13cH group), dynamization 13cH, in male Swiss mice at 28 days of age. On days 0 and 12 after infection (a.i.), the BSNI 13cH group exhibited a pronounced Th1 response that was attributable to a reduction of interleukin-4 (IL-4) concentrations, with no significant differences in interferon-γ (IFN-γ) concentrations and a decrease in IL-17A concentrations on day 0 a.i. compared with the control and BSI 13cH groups. However, this cytokine balance was not sufficient to alter blood parasitemia in treated animals, likely because of a decrease in IFN-γ concentrations on day 8 a.i., thus hindering a more effective Th1 response. In contrast, the BSI 13cH group presented a pronounced Th2 response that was attributable to an increase in IL-4 concentrations (on days 0 and 8 a.i.) and a decrease in IFN-γ concentration (on day 12 a.i.) compared with the control and BSNI 13cH groups. This cytokine balance suppressed the immune response to T. cruzi in murine infection, resulting in a significant increase in blood parasitemia, decrease in the patent period and subsequently a decrease in survival time. The results indicate that these highly diluted medications differentially modulate the immune system and represent a substantial contribution to the field of homeopathic medicine, providing evidence of the action of these medications.

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In vivo efficacy of a biotherapic and eugenol formulation against Rhipicephalus microplus

et al. 2017

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The control of Rhipicephalus microplus is essential to prevent cattle discomfort and economic losses. However, increased resistance and acaricides inefficiency lead producers to adopt strategies that could result in the accumulation of chemical residues in meat and milk with possibilities of poisoning in animals and people. This scenario demonstrates the necessity of research into the identification of novel, effective and environmentally safe therapeutic options for cattle tick control. The objectives of this study were to develop and assess the efficacy of R. microplus biotherapic and of 5% eugenol for the control of R. microplus in artificially infested calves. Eighteen male 6-month-old Holstein calves were divided into three groups of six animals. In Group 1, the animals did not receive medication (control group); in Group 2, the animals received 1 mL of R. microplus biotherapic at dilution 6CH (centesimal Hahnemannian), orally administered twice daily. And in Group 3, they received a single application of eugenol 5% in the pour-on formulation. The median efficacy for biotherapy and eugenol 5% was respectively 10.13 and 13.97%; however, upon analyzing reproductive efficiency, it is noteworthy that the biotherapic had 45.86% efficiency and was superior to the action of eugenol (12.03%) after 37 days of treatment. The ultrastructural study provided information about the effects of R. microplus biotherapic on the ovaries of engorged females and showed disorganization in the deposition of the oocyte exochorion. The results suggest hatchability inhibition of larvae, interference in R. microplus reproduction and future possibilities for eco-friendly control of R. microplus with biotherapic 6CH.

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Different forms of administration of biotherapy 7dH in mice experimentally infected by Trypanosoma cruzi produce different effects

Cited by 27 publications

References 24 publications

Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi

Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi

Modulation of IFN-γ, IL-4 and IL-17 Cytokines is Related to Parasitemia Control in Mice Infected by <i>Trypanosoma cruzi</i> and Treated with Biotherapy

In vivo efficacy of a biotherapic and eugenol formulation against Rhipicephalus microplus

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Different forms of administration of biotherapy 7dH in mice experimentally infected by Trypanosoma cruzi produce different effects

Cited by 27 publications

References 24 publications

Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi

Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi

Modulation of IFN-&gamma;, IL-4 and IL-17 Cytokines is Related to Parasitemia Control in Mice Infected by <i>Trypanosoma cruzi</i> and Treated with Biotherapy

In vivo efficacy of a biotherapic and eugenol formulation against Rhipicephalus microplus

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Modulation of IFN-γ, IL-4 and IL-17 Cytokines is Related to Parasitemia Control in Mice Infected by <i>Trypanosoma cruzi</i> and Treated with Biotherapy