Different responses of low grain-Cd-accumulating and high grain-Cd-accumulating rice cultivars to Cd stress

Wang, Feijuan; Wang, Min; Liu, Zhouping; Shi, Yan; Han, Tiqian; Ye, Yaoyao; Gong, Ning; Sun, Junwei; C, Zhu

doi:10.1016/j.plaphy.2015.08.001

Cited by 74 publications

(28 citation statements)

References 29 publications

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“…SOD activity, CAT activity and POD activity were assayed according to Ma et al [32] and Wang et al [33]. 0.3 g fresh rice leaves samples were ground with liquid nitrogen, and then added with 3 ml cold buffer (50 mM PBS pH 7.0, 3 mM DTT, 1 mM EDTA-Na 2 , 5% PVP).…”

Section: Extraction and Determination Of Superoxide Dismutase (Sod) mentioning

confidence: 99%

A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

et al. 2019

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Background: Cd is one of the highly toxic heavy metals to most organisms, including humans and plants, and Cd-contaminated rice from China has become a global food safety issue. The early prediction of OsPCR (the plant cadmium resistance protein) which contained a PLAC8 domain was related with the accumulation of Cd in rice. To further understand the biological function of the OsPCR genes on the Cd tolerance and Cd accumulation in rice, we used a low grain-Cd-accumulating rice (xiushui 11) and a high grain-Cd-accumulating rice (xiushui 110) varieties to analyze the relationship between the expression levels of the two most abundant expression genes (OsPCR1 and OsPCR3) and the Cd concentrations in different tissues at different growth periods during Cd stress, and transgenic experiments of OsPCR1 and OsPCR3 were carried out. Results: OsPCR1 and OsPCR3 were closely related with Cd accumulation. Overexpression of OsPCR1 and OsPCR3 could not only increase the Cd tolerance, but also decrease the Cd accumulation obviously in different parts of the transgenic rice plants (especially in the rice grains), while the RNAi expression plants showed the opposite results. Conclusions: These results indicate that OsPCR1 and OsPCR3 play critical roles in Cd accumulation in rice, which provides a theoretical basis for the safe production of rice.

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Section: Extraction and Determination Of Superoxide Dismutase (Sod) mentioning

confidence: 99%

A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

et al. 2019

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“…In previous studies (He et al 2014;Wang et al 2015), the MDA concentrations in the rice shoot increased signi cantly with the treatment of high Cd levels (i.e., 100-500 mM). Xie et al (2015) reported that the MDA observed in the shoot of rice seedlings treated with low Cd concentrations of ~40 mM for 15 days increased by up to two times compared with that in the control.…”

Section: Characterization Of the Oxidative Statuses In Rice Plants Unmentioning

confidence: 78%

“…Nevertheless, root-to-shoot Cd translocation is the major process determining grain Cd accumulation (Uraguchi and Fujiwara 2012). Wang et al (2015) emphasized that stem transportation may play an important role in grain Cd accumulation, which was combined with the expression level of the OsPCR1 gene. Lower Cd translocation from the root to the shoot may result in lower grain Cd accumulation.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, so far few concerns have been raised about the genotypic variations in Cd distribution and translocation in rice plants owing to physiological mechanisms for Cd tolerance (Mohamed et al 2012;Xie et al 2015). Wang et al (2015) tried to identify genotypic variations in Cd content in rice grains depending on the physiological mechanisms for Cd tolerance. They suggested that rice cultivars have different Cd detoxi cation and accumulation mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Aspects of cultivar variation in physiological traits related to Cd distribution in rice plants with a short-term stress

Chiao¹,

Chen

Syu

et al. 2020

Preprint

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Background Genotypic variations are seen in cadmium (Cd) tolerance and accumulation in rice plants. Cultivars that show low Cd translocation from the root into shoot can be selected to reduce Cd contamination in rice grains. This study aims to clarify the physiological regulation related to Cd absorption by rice plants for screening out the cultivars, which have relatively low Cd accumulation in grains. Eight Taiwan mega cultivars of paddy rice: japonica (TY3, TK9, TNG71, and KH145 cultivars), indica (TCS10 and TCS17 cultivars), and glutinous (TKW1 and TKW3 cultivars), which are qualified with the criteria for rice grain quality by the Council of Agriculture, Taiwan, were used for illustration. An experiment in hydroponics was conducted for the rice seedlings with a treatment of 50 μM CdCl 2 for 7 days. Results and discussion After the Cd treatment, the reductions in shoot growth were more significant than those in root growth; however, Cd absorbed in the rice plant was sequestered much more in the root. The malondialdehyde (MDA) was preferentially accumulated in rice root but the hydrogen peroxide (H 2 O 2 ) was increased more significantly in the shoot; the antioxidative enzymes, superoxide dismutase (SOD) and ascorbate peroxidase (APX), were pronounced more in rice shoot. Conclusions The rice cultivars preferentially accumulated Cd in the root rather than the shoot with the Cd treatment, which resulted in significant enhancements of MDA and growth reductions in the root. However, H 2 O 2 accumulation was toward the shoot to retard shoot growth suddenly and then the root could keep a gradual growth. Also, the rice cultivars, which preferentially accumulate Cd in the root, would have the regulation tendency of SOD toward the shoot. Due to that SOD is responsible for H 2 O 2 production, H 2 O 2 accumulation would be thus toward the shoot. Moreover, the cultivars, which have a less regulation tendency of APX toward the shoot, would present higher translocation of Cd into the shoot.

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“…In this work, we showed that Onzin, the reference for PLAC8 cell growth regulators [2], could induce cadmium resistance in S. cerevisiae , demonstrating that these two functions are not mutually exclusive in PLAC8 proteins. Another example of how the same PLAC8 protein can display both functions is the rice OsPCR1 that, even though with different acronyms, has been reported as cell growth regulator [16] and able to confer cadmium-resistance [65].…”

Section: Resultsmentioning

confidence: 99%

Expression of mammalian Onzin and Fungal Cadmium Resistance 1 in S. cerevisiae suggests ancestral functions of PLAC8 proteins in regulating mitochondrial metabolism and DNA damage repair

Daghino

Vietro

Petiti

et al. 2018

Preprint

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22Protein domains are structurally and functionally distinct units responsible for particular protein 23 functions or interactions. Although protein domains contribute to the overall protein function(s) and 24 can be used for protein classification, about 20% of protein domains are currently annotated as 25 "domains of an unknown function" (DUFs). DUF 614, a cysteine-rich domain better known as 26 PLAC8 (Placenta-Specific Gene 8), occurs in proteins found in the majority of Eukaryotes. containing proteins play important yet diverse roles in different organisms, such as control of cell 28 proliferation in animals and plants or heavy metal resistance in plants and fungi. For example, 29 Onzin from Mus musculus is a key regulator of cell proliferation, whereas FCR1 from the 30 ascomycete Oidiodendron maius confers cadmium resistance. Onzin and FCR1 are small, single-31 domain PLAC8 proteins and we hypothesized that, despite their apparently different role, a 32 common molecular function of these proteins may be linked to the PLAC8 domain. To address this 33 hypothesis, we compared these two PLAC8-containing proteins by heterologous expression in the 34 PLAC8-free yeast Saccharomyces cerevisiae. When expressed in yeast, both Onzin and FCR1 35 improved cadmium resistance, reduced cadmium-induced DNA mutagenesis, localized in the 36 nucleus and induced similar transcriptional changes. Our results support the hypothesis of a 37 common ancestral function of the PLAC8 domain that may link some mitochondrial biosynthetic 38 pathways (i.e. leucine biosynthesis and Fe-S cluster biogenesis) with the control of DNA damage, 39 thus opening new perspectives to understand the role of this protein domain in the cellular biology 40 of Eukaryotes.41 42 Author Summary 43Protein domains are the functional units of proteins and typically have distinct structure and 44 function. However, many widely distributed protein domains are currently annotated as "domains of 45 unknown function" (DUFs). We have focused on DUF 614, a protein domain found in many 46 Eukaryotes and better known as PLAC8 (Placenta-Specific Gene 8). The functional role of DUF 47 614 is unclear because PLAC8 proteins seem to play important yet different roles in taxonomically 48 distant organisms such as animals, plants and fungi. We used S. cerevisiae to test whether these 49 apparently different functions, namely in cell proliferation and metal tolerance, respectively 50 reported for the murine Onzin and the fungal FCR1, are mediated by the same molecular 51 mechanisms. Our data demonstrate that the two PLAC8 proteins induced the same growth 52 phenotype and transcriptional changes in S. cerevisiae. In particular, they both induced the 53 biosynthesis of the amino acid leucine and of the iron-sulfur cluster, one of the most ancient protein 54 cofactors. These similarities support the hypothesis of an ancestral function of the DUF 164 55 domain, whereas the transcriptomic data open new perspectives to understand the role of PLAC8-56 proteins in Eukaryotes.57...

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Different responses of low grain-Cd-accumulating and high grain-Cd-accumulating rice cultivars to Cd stress

Cited by 74 publications

References 29 publications

A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

Aspects of cultivar variation in physiological traits related to Cd distribution in rice plants with a short-term stress

Expression of mammalian Onzin and Fungal Cadmium Resistance 1 in S. cerevisiae suggests ancestral functions of PLAC8 proteins in regulating mitochondrial metabolism and DNA damage repair

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