Different roles of retinal dopamine in albino Guinea pig myopia

Mao, Junfeng; Liu, Shuangzhen

doi:10.1016/j.neulet.2016.12.061

Cited by 24 publications

(16 citation statements)

References 12 publications

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“…29 A recent report showed that systemic administration of L-DOPA to AM guinea pigs reduces both spontaneous myopia and form-deprived myopia. 30 Guinea pigs have no retinal blood vessels, 31 and the retinal DA levels in AM and PH guinea pigs were similar. Therefore, we speculate that systemic injection of L-DOPA increased DA levels in the RPE/choroid, thereby inhibiting myopic changes by way of the proposed supplemental uveal dopaminergic pathway.…”

Section: Involvement Of the Rpe/choroidal Tyrosinase-dependent Dopamimentioning

confidence: 85%

Effects of the Tyrosinase-Dependent Dopaminergic System on Refractive Error Development in Guinea Pigs

Jiang

Zhang

Chen

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To determine if myopia in albino guinea pigs is linked to altered ocular dopamine (DA) levels in both the retinal and uveal dopaminergic systems. METHODS. Retina and retinal pigment epithelium (RPE)/choroid were dissected from eyes of 2week-old albino myopic (AM) and pigmented hyperopic (PH) guinea pigs. The levels of DA, dihydroxy-phenyl acetic acid (DOPAC), and homovanillic acid (HVA) were determined. Tyrosine hydroxylase (TH) and tyrosinase activities were also measured. PH animals received daily unilateral peribulbar injections of either kojic acid (tyrosinase inhibitor) or vehicle for 2 to 4 weeks. Refractive errors and ocular axial dimensions were measured by eccentric infrared photoretinoscopy and A-scan ultrasonography. RESULTS. Retinal DA levels were similar between the two strains, but AM eyes had higher levels of DOPAC. RPE/choroid DA and tyrosinase activity in AM eyes were lower than in PH eyes (P < 0.01); however, the DA turnover was higher (P < 0.05). After 2 weeks of kojic acid treatment, PH eyes developed significant myopia, accompanied by elongated vitreous chambers and axial lengths. Inhibition of tyrosinase activity was linearly correlated with a myopic refraction shift (R ¼ 0.79, P < 0.01). PH animals that received 62.5 ng/mL kojic acid treatment daily for 2 weeks followed by 625 ng/mL for 2 more weeks became more myopic and had deeper anterior chambers compared to those that received the 62.5 ng/mL dose over this period (P ¼ 0.04). CONCLUSIONS. The uveal tyrosinase-dependent dopaminergic system is involved in the development of guinea pig refraction. Enhancing uveal tyrosinase activity might slow down the development of myopia.

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Section: Involvement Of the Rpe/choroidal Tyrosinase-dependent Dopamimentioning

confidence: 85%

Effects of the Tyrosinase-Dependent Dopaminergic System on Refractive Error Development in Guinea Pigs

Jiang

Zhang

Chen

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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“…; Luo et al. ; Mao & Liu ). The dopamine system in the retina has extensively been studied for its potential role in the inhibition of myopia (Stone et al.…”

Section: Discussionmentioning

confidence: 99%

“…; Yan et al. ; Mao & Liu ). In the lens‐induced myopia model, an intravitreal application of a non‐specific dopaminergic agonist (apomorphine) in a dose of 10 nmol/20 μ l resulted in an ocular growth inhibition (Nickla et al.…”

Section: Discussionmentioning

confidence: 99%

Amphiregulin and ocular axial length

Dong

Kang

et al. 2019

Acta Ophthalmologica

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Purpose: To assess the potential role of amphiregulin as messenger molecule in ocular axial elongation. Methods: The experimental study included guinea pigs (total n = 78) (age: 3-4 weeks) which underwent bilateral lens-induced myopization and received 15 days later three intraocular injections in weekly intervals of amphiregulin antibody (doses:5 lg, 10 lg, 20 lg) into their right eyes, and three phosphatebuffered saline injections into their left eyes; and guinea pigs without lens-induced myopization and which received three unilateral intraocular injections of amphiregulin antibody (dose: 20 lg) or amphiregulin (doses: 1 ng; 10 ng; 20 ng) into their right eyes, and three phosphate-buffered saline injections into their left eyes. Seven days later, the animals were sacrificed. Intravitally, we performed biometry, and histology and immunohistochemistry post-mortem. Results: In animals with bilateral lens-induced myopization, the right eyes receiving amphiregulin antibody showed reduced axial elongation in a dose-dependent manner (dose: 5 lg: side difference: 0.14 AE 0.05 mm;10 lg: 0.22 AE 0.06 mm; 20 lg: 0.32 AE 0.06 mm; p < 0.001), thicker sclera (all p < 0.05) and higher cell density in the retinal nuclear layers and retinal pigment epithelium (RPE) (all p < 0.05). In animals without lens-induced myopia, the right eyes with amphiregulin antibody application (20 lg) showed reduced axial elongation (p = 0.04), and the right eyes with amphiregulin injections experienced increased (p = 0.02) axial elongation in a dosedependent manner (1 ng: 0.04 AE 0.06 mm; 10 ng: 0.10 AE 0.05 mm; 20 ng: 0.11 AE 0.06 mm). Eyes with lens-induced axial elongation as compared to eyes without lens-induced axial elongation revealed an increased visualization of amphiregulin upon immunohistochemistry and higher expression of mRNA of endogenous amphiregulin and epidermal growth factor receptor, in particular in the outer part of the retinal inner nuclear layer and in the RPE. Conclusion: Amphiregulin may be associated with axial elongation in young guinea pigs.

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“…Previously, the retinal samples were collected under dim light, which may have led to inclusion of DA from the choroidal debris. 8 To minimize choroidal contamination in the current study, retinal samples were collected in a well-lighted room rather than under dim light. However, increasing the light intensity and spending additional time to remove choroidal debris may lower DA levels below those previously reported, because light and air are detrimental to the stability of DA.…”

Section: Discussionmentioning

confidence: 99%

“…6 Similarly, supplementation with L-DOPA, a DA precursor, inhibited FDM in pigmented guinea pigs 7 and spontaneous myopia in their albino counterpart. 8 In addition, the nonselective dopamine receptor (DR) agonist apomorphine (APO) inhibited myopia in chickens, 4 guinea pigs, 5 and rhesus monkeys. 9 Despite DA-level invariance during FD in mice, 10 DR involvement remains plausible, because APO inhibited myopia in this species.…”

mentioning

confidence: 99%