Different signalling pathways contribute to the control of GPD1 gene expression by osmotic stress in Saccharomyces cerevisiae

Hyperosmotic Stress Stimulates Promoter Activity and Regulates Cellular Utilization of the Serum- and Glucocorticoid-inducible Protein Kinase (Sgk) by a p38 MAPK-dependent Pathway

“…Sp1 is a Cys 2 His 2 zinc finger-binding protein that has been shown to bind specifically to the GC-rich GCCCCGCCCC sequence (42, 45) that we defined as the hyperosmotic stress-regulated ele- ment in the sgk promoter. Our studies are generally consistent with previous observations in the yeast S. cerevisiae in which the Msn family of transcription factors are targeted by the yeast p38 MAPK homologue HOG1 and bind to a CCCCT DNA sequence that is similar to the Sp1 binding core sequence (7,8,10). In earlier studies, Sp1 was viewed as a component of the basal transcriptional machinery.…”

“…The HOG1 gene is necessary for the maintenance of osmotic gradients in hyperosmolar environments and allows the proliferation of yeast under osmotic stress (6). HOG1 has been shown to phosphorylate various members of the Msn family of transcription factors, resulting in their binding to and transactivation of stress-responsive elements located in the upstream promoter regions of osmotically regulated genes (7)(8)(9)(10). One class of osmoregulatory genes targeted by HOG1 encode proteins that have osmoprotective functions, such as the glycerol-3-phosphate dehydrogenase gene that is responsible for the accumulation of the yeast osmolyte, glycerol (10,11).…”

Dynamic Changes in the Subcellular Distribution of Gpd1p in Response to Cell Stress

“…In addition, GPD1 expression is altered by a wide variety of stresses, including heat, cold, and oxidative stress (16 -18), suggesting its regulation is controlled by stress. Genome-wide monitoring of transcript changes in yeast under various stress conditions also showed that GPD1 belongs to a large group of common stressresponse genes (19,20); however, its regulation is complex and appears to be controlled by multiple signaling pathways (21). This likely reflects the multiple metabolic changes that yeast cells undergo in response to different stresses (19,22,23) and the function of Gpd1p at the interface of many metabolic pathways, including glycolysis, glycerolipid and phospholipid biosynthesis, and glycerol metabolism (8 -12).…”

Adaptación a estrés osmótico en Saccharomyces cerevisiae: Caracterización genómica de factores de transcripción involucrados y represión de la biosíntesis de ergosterol

“…Sí se sabía que había varios factores de transcripción involucrados y sólo unos pocos genes cuya estimulación por estrés osmótico es controlada exclusivamente por la ruta HOG; además, los promotores a los que se unen, como los de los genes GPD1 (codifica la deshidrogenasa glicerol-3-fosfato) y ENA1 (codifica la principal bomba exportadora de sodio), parecen ser muy complejos (Eriksson et al, 2000;Proft and Serrano, 1999;Rep et al, 1999a).…”

“…Sí se sabía que había varios factores de transcripción involucrados y sólo unos pocos genes cuya estimulación por estrés osmótico es controlada exclusivamente por la ruta HOG; además, los promotores a los que se unen, como los de los genes GPD1 (codifica la deshidrogenasa glicerol-3-fosfato) y ENA1 (codifica la principal bomba exportadora de sodio), parecen ser muy complejos (Eriksson et al, 2000;Proft and Serrano, 1999;Rep et al, 1999a). Además, el sistema de cascada de fosforilaciones Sln1p-Ypd1p controla otro regulador, Skn7p, involucrado en múltiples procesos celulares y relacionado tanto a la ruta HOG como a la ruta de integridad celular, aunque su principal función fisiológica está relacionada con la adaptación a estrés oxidativo (Krems et al, 1996;Lee et al, 1999;Morgan et al, 1997).…”