Different Sp1 family members differentially affect transcription from the human elongation factor 1 A-1 gene promoter

Nielsen, S. J.; Præstegaard, Morten; Jorgensen, H. F.; Clark, Brian F. C.

doi:10.1042/bj3330511

Cited by 29 publications

(16 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is reasonable to expect they also compete for binding to promoters containing GC-boxes. In fact, these two factors have been shown to display differential activity, including parallel or opposing transcription effects depending on the promoter (Conn et al 1996, Kennett et al 1997, Hata et al 1998, Hoppe & Francone 1998, Nielsen et al 1998, Rajakumar et al 1998, Yajima et al 1998, Merchant et al 1999, Vines & Weigent 2000. Cell-type-specific levels of Sp1 and Sp3 variants have the potential to be an important mode of regulating the contribution of Sp1 to promoter activity.…”

Section: Role Of Sp1 In Basal Transcriptionmentioning

confidence: 99%

Role of Sp1 in insulin regulation of gene expression

Samson

Wong²

2002

Journal of Molecular Endocrinology

142

View full text Add to dashboard Cite

Sp1 is a ubiquitous nuclear factor that plays a key role in maintaining basal transcription of 'house-keeping' genes. However, recent evidence points to a more important function for Sp1 in mediating 'cross-talk' between selected signaling cascades to regulate the target genes that respond to these pathways. The role of Sp1 in mediating the actions of the peptide hormone insulin is of specific interest and serves as a model for detailing effects of intracellular signaling on Sp1 activity. This review summarizes studies suggesting that changes in Sp1 phosphorylation provide one potential mechanism for manipulating activity of this protein. A growing body of evidence reveals that the DNA binding and transcription activity of Sp1 may increase or decrease in response to changes in phosphorylation. This enables 'fine-tuning' of Sp1 activity for regulation of gene transcription. Several mechanisms exist by which Sp1 alters gene activity in response to insulin. These include independent Sp1 activity as well as collaboration or competition with others factors. This review points to an ever-increasing role for Sp1 in regulating the transcription of genes in response to extracellular signals such as insulin.

show abstract

Section: Role Of Sp1 In Basal Transcriptionmentioning

confidence: 99%

Role of Sp1 in insulin regulation of gene expression

Samson

Wong²

2002

Journal of Molecular Endocrinology

142

View full text Add to dashboard Cite

show abstract

“…A second type of Sp1/Sp3 responsive promoter includes human Id4 (Pagliuca et al, 1998), human POLD1 (Zhao and Chang, 1997), human elongation factor 1 A-1 (Nielsen et al, 1998), human glucagonlike peptide-1 receptor (Wildhage et al, 1999), human endothelial nitric-oxide synthase (Karantzoulis-Fegaras et al, 1999) and neuronal nicotinic acetylcholine receptor b4 subunit (Bigger et al, 1997) gene promoters, which are positively regulated by both Sp1 and Sp3. Our data suggest that the p21 promoter belongs to this group and that Sp3 is a stronger positive regulator of the p21 promoter than Sp1.…”

Section: Sp1 and Sp3 Bind To The P21 Promoter In Caco-2 Cellsmentioning

confidence: 99%

Sp1 and Sp3 activate p21 (WAF1/CIP1) gene transcription in the Caco-2 colon adenocarcinoma cell line

et al. 2000

View full text Add to dashboard Cite

The CDK inhibitor p21 WAF1/CIP1 is a negative regulator of the cell cycle, and its expression is induced during terminal dierentiation in vitro and in vivo. Expression of p21 is controlled at the transcriptional level by both p53-dependent and -independent mechanisms. Our previous studies established that p21 is expressed in the Caco-2 adenocarcinoma cell line, and its expression is induced by a p53-independent mechanism during dierentiation of these cells. Here we have found that transcription of p21 in Caco-2 cells is controlled primarily by the transcription factors Sp1 and Sp3 through two Sp1 binding sites, Sp1-1 and Sp1-2, located between 7119 and 7114 bp and between 7109 and 7104 bp of the p21 promoter, respectively. Sp1 and Sp3 binding to the p21 promoter increased during Caco-2 cell dierentiation, while the absolute level of Sp1 did not change and the absolute level of Sp3 increased approximately twofold. Transfection experiments in the SL2 Drosophila cell line that lacks endogenous Sp3 activity demonstrated that Sp1 transactivates the p21 promoter primarily through the Sp1-2 site, while Sp3 acts through the Sp1-1 site. In these cells Sp3 is a stronger transactivator of the p21 promoter than Sp1. Our data suggest that induction of p21 transcription during Caco-2 dierentiation is modulated by Sp1/Sp3 interactions with the p21 promoter. Oncogene (2000) 19, 5182 ± 5188.

show abstract

“…To avoid increased coreporter activity due to cytokine-or LPS-mediated stimulation of CMV promoter, pRL-EF1␣ was used as a coreporter construct (24). Renilla expression in pRL-EF1␣ is driven by the strong human elongation factor 1␣1 (EF1␣) gene promoter regulated mainly by GC box-binding transcription factors (34). Reporter activities are calculated as ratio of firefly luciferase test construct activity divided by activity of cotransfected Renilla luciferase reporter construct.…”

Section: Transient Cell Transfection and Luciferase Assaymentioning

confidence: 99%

Differential Regulation of CCL22 Gene Expression in Murine Dendritic Cells and B Cells

Ghadially

Ross

Kerst

et al. 2005

The Journal of Immunology

View full text Add to dashboard Cite

The activated T cell-attracting CC chemokine CCL22 is expressed by stimulated B cells and mature dendritic cells (DC). We have cloned and sequenced the complete mouse gene, including 4 kb of the 5′-flanking promoter region, and detected two distinct sites for initiation of transcription by 5′-RACE. Reporter gene assays indicate that the promoter reflects the specificity of the endogenous gene. Within the proximal promoter region, we identified potential binding sites for NF-κB, Ikaros, and a putative GC box. All three regions bind proteins. The NF-κB site was shown to specifically bind NF-κB subunits p50 and p65 from nuclear extracts of LPS-stimulated B cells, B cell line A20/2J, TNF-α-stimulated bone marrow-derived DC, and DC line XS106. Furthermore, promoter activity was affected by targeted mutagenesis of the NF-κB site and transactivation with p50 and p65. The region harboring the putative Ikaros site contributes to promoter activity, but the binding protein does not belong to the Ikaros family. The GC box was shown to specifically bind Sp1 using extracts from LPS-stimulated B cells and A20/2J but not from DC and DC line XS106. Additionally, Sp1 transactivated the promoter in A20/2J but not in XS106 cells, and mutation of the Sp1 site diminished transactivation. Furthermore, binding of the protein complex at the GC box is required for NF-κB activity, and the spatial alignment of the binding sites is of critical importance for promoter activity. Thus, identical and distinct proteins contribute to expression of CCL22 in DC and B cells.

show abstract

Different Sp1 family members differentially affect transcription from the human elongation factor 1 A-1 gene promoter

Cited by 29 publications

References 62 publications

Role of Sp1 in insulin regulation of gene expression

Role of Sp1 in insulin regulation of gene expression

Sp1 and Sp3 activate p21 (WAF1/CIP1) gene transcription in the Caco-2 colon adenocarcinoma cell line

Differential Regulation of CCL22 Gene Expression in Murine Dendritic Cells and B Cells

Contact Info

Product

Resources

About