Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development

Pollen cells are symplasmieally isolated during maturation and germination. Pollen therefore needs to take up nutrients via membrane carriers. Physiological measurements on pollen indicate sucrose transport in the pollen tube. A cDNA encoding a pollen-specific sucrose transporter-like protein NtSUT3 was isolated from a tobacco pollen cDNA library. NtSUT3 expression is detected only in pollen and is restricted to late pollen development, pollen germination and pollen tube growth. Altogether these data indicate that pollen is supplied not only with glucose, but also with sucrose through a specific sucrose transporter. The respective contribution of each transport pathway may change during pollen tube growth.

show abstract

“…To follow NtSUT3 expression during anther development, anthers were collected at the stages described in [26]. Fig.…”

Section: Developmental Regulation Oj Ntsut3 Expressionmentioning

confidence: 99%

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

Lemoine

Bürkle²,

Barker³

et al. 1999

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Samsun in bacteriophage 2 Charon 32 [12] was a generous gift of Dr R.B. Goldberg [15], Escherichia coli K802 cells were used as hosts. A c D N A of 240 bp comprising part of the 3'-end region was used to synthesize a random-primed probe labelled with [~-32p]dATP as described previously [9].…”

Section: Genomic Library Screeningmentioning

confidence: 99%

Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

View full text Add to dashboard Cite

The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM 19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM 19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species.

show abstract

“…Although the sequences of these genes have not been published yet, the radically different expression pattern makes it unlikely that they are identical to the genes described here. In addition, the tapetum-specific genes hybridize to different genomic fragments (Koltunow et al, 1990; Figure 2).…”

Section: Cell-type Specific Expressionmentioning

confidence: 99%

Expression pattern of a tobacco lipid transfer protein gene within the shoot apex

et al. 1992

View full text Add to dashboard Cite

SummaryA genomic clone coding for a putative lipid transfer protein was isolated from Nicotiana tabacurn. The gene is expressed in all aerial organs of the tobacco plant, with expression being highest in the upper part of the plant and declining towards the base (i.e. with increasing age). In the shoot apex transcript levels are highest in the LI layer, lower in the underlying cell layers, and are again higher in the submeristem region. This pattern is markedly different from those previously described for genes expressed within the shoot apex.

show abstract

Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development

Cited by 360 publications

References 0 publications

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

Isolation and characterization of a microspore-specific gene from tobacco

Expression pattern of a tobacco lipid transfer protein gene within the shoot apex

Contact Info

Product

Resources

About