Different timing and spatial separation of parental chromosomes in intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus

Ding, Li; Zhao, Zhigang; Ge, Xianhong; Li, Z Y

doi:10.4238/2014.april.8.3

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…In Glandularia Poggio et al (1993) proposed that genes affecting chromosome distribution in the nuclear membrane could modify the distance between homologous and/or homoeologous genomes. Recently we found evidence for spatial separation of haploid groups of five chromosomes in the hybrids (in preparation), a phenomena well documented in other plant groups ( Callimassia et al 1994 ; Ding et al 2014 ; Ran et al 2001 ). The separation of parental chromosomes potentially restricts homoeologous pairing and favors homologous pairing, resulting in balanced chromosome segregation in allopolyploids.…”

Section: Discussionsupporting

confidence: 65%

Variability in the amount of homoeologous pairing among F1 hybrids

Poggio¹,

Greizerstein

Ferrari

2015

AoB PLANTS

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Genes involved in the exclusive pairing of homologous chromosomes have been described in several polyploid species but little is known about the activity of these genes in diploids (which have only one dose of each homoeologous genome). Analysis of the meiotic behaviour of species, natural and artificial hybrids and polyploids of Glandularia suggests that, in allopolyploids where homoeologous genomes are in two doses, regulator genes prevent homoeologous pairing. The different meiotic phenotypes in diploid F1 hybrids between Glandularia pulchella and Glandularia incisa strongly suggest that these pairing regulator genes possess an incomplete penetrance when homoeologous genomes are in only one dose. Moreover, the meiotic analysis of natural and artificial F1 hybrids suggests that the genetic constitution of parental species influences the activity of pairing regulator genes and is mainly responsible for variability in the amount of homoeologous pairing observed in diploid hybrids. In Glandularia, the pairing regulator genes originated in South American diploid species. The cytogenetic characteristics of this genus make it a good model to analyse and explore in greater depth the activity of pairing regulator genes at different ploidy levels.

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Section: Discussionsupporting

confidence: 65%

Variability in the amount of homoeologous pairing among F1 hybrids

Poggio¹,

Greizerstein

Ferrari

2015

AoB PLANTS

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“…A disomic addition line with all the chromosomes of B. napus and one specific pair of O. violaceus chromosomes producing slightly red petals (designated M4) was obtained previously (Ding et al ., ; Zhao et al ., ). The M4 plant, the donor B. napus cv.…”

Section: Methodsmentioning

confidence: 99%

“…The crucifer Orychophragmus violaceus (L.) O. E. Schulz (2n=24, OO genomes), which has large purple flowers, is widely cultivated as an ornamental plant in China. One disomic addition line with all the chromosomes of B. napus and a specific pair of O. violaceus chromosomes produced flowers with a red colour (Ding et al ., ), suggesting that this foreign chromosome carried the key genes responsible for red petal colour. In this study, we used RNA‐Seq to perform comparative petal transcriptome profiling between the addition line, and the donors B. napus and O. violaceus , which have purple and white flowers.…”

Section: Introductionmentioning

confidence: 98%

Production of red‐flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2

Chen

Pan

et al. 2017

Plant Biotechnology Journal

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SummaryOilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower colour of oilseed rape. A previously established B. napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits slightly red petals. In this study, the transcriptomic analysis of M4, B. napus (H3), and O. violaceus with purple petals (OvP) and with white petals (OvW) revealed that most anthocyanin biosynthesis genes were up‐regulated in both M4 and OvP. Read assembly and sequence alignment identified a homolog of AtPAP2 in M4, which produced the O. violaceus transcript (OvPAP2). The overexpression of OvPAP2 via the CaMV35S promoter in Arabidopsis thaliana led to different levels of anthocyanin accumulation in most organs, including the petals. However, the B. napus overexpression plants showed anthocyanin accumulation primarily in the anthers, but not the petals. However, when OvPAP2 was driven by the petal‐specific promoter XY355, the transgenic B. napus plants produced red anthers and red petals. The results of metabolomic experiments showed that specific anthocyanins accumulated to high levels in the red petals. This study illustrates the feasibility of producing red‐flowered oilseed rape, thereby enhancing its ornamental value, via the ectopic expression of the OvPAP2 gene. Moreover, the practical application of this study for insect pest management in the crop is discussed.

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“…A study in Brachypodium distachyon root cells interphase nuclei showed that the association of homologous chromosomes is more frequent than expected in a random arrangement of all chromosomes within the nucleus 23 . The study of polyploid organisms like wheat and Brassica napus has shown that chromosomes of the different subgenomes are not intermingled but segregated, so that all chromosomes of a subgenome occupy a kind of genome territory, being the interactions among chromosomes of the same subgenome more probable and intense 24 . In the case of bread wheat, its genome includes three subgenomes (A, B, D) that would also occupy three different genome territories within the nucleus 25 .…”

Section: Discussionmentioning

confidence: 99%

Homologous chromosome associations in domains before meiosis could facilitate chromosome recognition and pairing in wheat

Barea

Redondo-Río

Lucena-Marín

et al. 2022

Sci Rep

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The increasing human population demands an increase in crop yields that must be implemented through breeding programmes to ensure a more efficient and sustainable production of agro-food products. In the framework of breeding, genetic crosses are developed between cultivated species such as wheat and their relative species that are used as genetic donors to transfer desirable agronomic traits into the crop. Unfortunately, interspecific associations between chromosomes from the donor species and the cultivar are rare during meiosis, the process to produce gametes in organisms with sexual reproduction, hampering the transfer of genetic variability into wheat. In addition, little is known about how homologous (equivalent) chromosomes initiate interaction and recognition within the cell nucleus to enter meiosis. In this context, we aim to get insight into wheat chromatin structure, particularly the distribution of homologous chromosomes within the cell nucleus and their putative interactions in premeiotic stages to facilitate chromosome associations and recombination at the beginning of meiosis. Cytogenetics allows the study of both the structure and the behaviour of chromosomes during meiosis and is key in plant breeding. In this study we visualized an extra pair of barley homologous chromosomes in a wheat genetic background to study the spatial distribution, arrangements and interactions occurring exclusively between this pair of homologous chromosomes during premeiosis using fluorescence in situ hybridization (FISH). Our results suggest that homologous chromosomes can initiate interactions in premeiotic stages that could facilitate the processes of specific chromosome recognition and association occurring at the onset of meiosis.

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Different timing and spatial separation of parental chromosomes in intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus

Cited by 9 publications

References 40 publications

Variability in the amount of homoeologous pairing among F1 hybrids

Variability in the amount of homoeologous pairing among F1 hybrids

Production of red‐flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2

Homologous chromosome associations in domains before meiosis could facilitate chromosome recognition and pairing in wheat

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