Different tumour grading in smokeless tobacco (Naswar) user and non-user in squamous cell carcinoma.

Sarfaraz, Amna; Rashid, Sahd; Irshad, Muhammad; Khattak, Maria Tasneem; Shabir, Hina

doi:10.29309/tpmj/2020.27.05.4278

Cited by 3 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this research had found that the majority of the cases were well differentiated (52.4%), followed by Moderately Differentiated cases (27.2%) and then poorly differentiated cases (20.4%). In accordance with our study Sarfaraz et al, 2020 showed well differentiated to be the most common grade for OSCC cases (19). Contrary to our Results Mehdi et al, 2019 from Pakistan showed same number of moderately differentiated and poorly differentiated cases and slightly less number of well differentiated cases (20).…”

Section: Resultssupporting

confidence: 91%

Expression of P16 in Various Grades of Oral Squamous Cell Carcinoma of Tertiary Care Hospitals of Peshawar

Ali¹,

Alam²,

Khan³

et al. 2022

PJMHS

View full text Add to dashboard Cite

Introduction: Oral squamous carcinoma (OSCC) is one of the ten most common carcinomas in the world. The survival rate of OSSC is poor due to its diagnosis at advanced stages, The main function of p16 in the body is to halt the tumor process, but genetic mutation may result in the development of OSCC. Objectives: The aim and objective was to compare the immunohistochemical expression of p16 in various grades of oral squamous cell carcinoma. Materials and Methods: This study was a retrospective comparative cross-sectional (Analytical) study. It was conducted at the Department of Pathology, Peshawar Medical College from August 2020 to August 2021. The sample size was calculated through G Power and 103 patients were included in the study. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS) version 20.0. Chi-Square test was performed for categorical variables. Results: It was observed in our study that the mean age of OSCC was 59.16 ± 13 SD. The commonest effected age was 61-70 years (29%). The male to female ratio was approximately 1.2:1. The most common site involved by OSCC was buccal mucosa (n=49, 47%) followed by the tongue (n = 29, 29%). The p16 expression was positive in most OSCC were well differentiated (n=50, 92.5%), followed by moderately differentiated cases (n=26, 92.8%) and poorly differentiated (n=81, cases, 85.7%). There was no significant difference for p16 expression and grades of OSCC cases (P-value =0.601). Conclusion:The expression of p16 was more common in Well-differentiated oral squamous cell carcinoma, followed by moderately and poorly differentiated OSCC.

show abstract

Section: Resultssupporting

confidence: 91%

Expression of P16 in Various Grades of Oral Squamous Cell Carcinoma of Tertiary Care Hospitals of Peshawar

Ali¹,

Alam²,

Khan³

et al. 2022

PJMHS

View full text Add to dashboard Cite

show abstract

“…Naswar is a combination of crushed tobacco leaves, slaked lime, and ashes, which is either inhaled or dipped in the vestibule of either side. After 5 min, the nicotine-associated products seep into the inner mucosa and exhibit a slight burning and numbing sensation [ 21 ]. The effect is mainly due to the high pH of un-ionized nicotine as well as tobacco-specific N-nitrosamines (TSNAs), which are carcinogenic and genotoxic.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Unstimulated Whole Salivary Tumor Necrosis Factor Alpha (TNF-α) and Cellular Micronuclei Levels in Snuff (Naswar) Users and Non-Users for Early Diagnosis of Oral Squamous Cell Carcinoma

Waqar

Khan

Zafar

et al. 2021

IJERPH

View full text Add to dashboard Cite

The aim of the study was to investigate the unstimulated whole saliva (UWS) tumor necrosis factor alpha (TNF-α) and cellular micronuclei in snuff dippers (Naswar) compared to healthy control subjects. The case control study was conducted over 9 months at a tertiary care center. Sixty patients were divided into two groups: Snuff dippers (SD) (Naswar) and non-snuff dippers (NSD) (control subjects). The included self-reported SD used Snuff twice daily for more than 12 months. UWS was collected and salivary TNF-α assessment was performed using enzyme-linked immunosorbent assay (ELISA). For cellular micronuclei, buccal mucosa was brushed to obtain cells in Naswar users, fixed with a dibutylphthalate polystyrene xylene (DPX) mounting to view micronuclei. Means and standard deviations were compared using the t-test and outcomes were related using Pearson correlation, considering p ≤ 0.05 as significant. The mean age of participants was 38.85 ± 11.56 years. The mean duration of snuff use was 20.43 ± 12.79 years and the common site for Naswar placement was the lower vestibule (n = 19, 63.3%). TNF-α levels among SD were 9.6 ± 3.3 pg/mL, which were significantly higher than levels in NSD, 5.2 ± 3 pg/mL (p < 0.05). The number of cellular micronuclei in SD was 30.7 ± 7.8, which was comparatively higher than in NSD, which was 9.2 ± 3.3 (p < 0.05). The duration of snuff use was positively correlated to TNF-α levels (p = 0.048) rather than the micronuclei number (p = 0.97). SD showed higher levels of TNF-α and cellular micronuclei compared with NSD (control subjects); a positive correlation was shown with the duration of snuff use. We conclude that TNF-α and micronuclei are potential salivary biomarkers for an oral biological effect in snuff (Naswar) users.

show abstract

Immunohistochemical (IHC) Expression of p16 in Various Grades of Oral Squamous Cell Carcinoma (OSCC) with Snuff Use in Tertiary Care Hospitals of Peshawar

Ali,

Alam,

Kamran

et al. 2024

PJHS

View full text Add to dashboard Cite

Oral squamous cell carcinoma (OSCC) is a significant health concern, with various factors influencing its development and progression. Understanding these factors, including p16 expression and clinicopathologic features, is crucial for improved diagnosis and treatment. Objective: To compare various grades of OSCC based on immunohistochemical expression of p16 and clinicopathologic parameters. Methods: The comparative cross-sectional study was conducted at the Department of Pathology, Peshawar Medical College (PMC), and Peshawar Dental College (PDC) from August 2020 to August 2021. It included 53 cases of OSCC with documented snuff use history. Tumor sections were stained with Hematoxylin and Eosin and underwent immunohistochemical staining for p16 expression. Sample size calculation utilized G Power software. Statistical analysis was performed using SPSS version 20.0, employing the Chi-Square test to assess categorical variables. Results: Among the 53 OSCC cases, the majority were male (66.0%) with ages ranging from 26 to 85 years, the most common age group being 51-70 years. The tongue was the primary site for OSCC development. Well-differentiated cases were predominant (64.2%), followed by moderate (20.8%) and poor grade cases (15.1%). However, no significant association was found between p16 expression and OSCC grades. Notably, p16 expression tended to be higher in snuff users and well-differentiated OSCC cases, although not statistically significant. Conclusions: Well-differentiated OSCC cases exhibited the highest expression of p16, followed by moderate and poorly differentiated cases. However, no significant correlation was observed between p16 expression and OSCC in snuff users.

show abstract

Different tumour grading in smokeless tobacco (Naswar) user and non-user in squamous cell carcinoma.

Cited by 3 publications

References 12 publications

Expression of P16 in Various Grades of Oral Squamous Cell Carcinoma of Tertiary Care Hospitals of Peshawar

Expression of P16 in Various Grades of Oral Squamous Cell Carcinoma of Tertiary Care Hospitals of Peshawar

Assessment of Unstimulated Whole Salivary Tumor Necrosis Factor Alpha (TNF-α) and Cellular Micronuclei Levels in Snuff (Naswar) Users and Non-Users for Early Diagnosis of Oral Squamous Cell Carcinoma

Immunohistochemical (IHC) Expression of p16 in Various Grades of Oral Squamous Cell Carcinoma (OSCC) with Snuff Use in Tertiary Care Hospitals of Peshawar

Contact Info

Product

Resources

About