Differential Accumulation of Soybean Seed Storage Protein Subunits in Response to Sulfur and Nitrogen Nutritional Sources

Paek, Nam‐Chon; Sexton, Peter; Naeve, Seth L.; Shibles, Richard

doi:10.1626/pps.3.268

Cited by 34 publications

(18 citation statements)

References 30 publications

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“…Table 1). Besides, most of those proteins are differentially expressed between the two cultivars during grain lling, indicating that the enhancement of the primary metabolism to facilitate grain lling which is consistent with previous studies [42][43][44][45][46] that revealed that the upregulation of metabolic proteins enhances seed development. The abundance of some metabolic proteins in plants such as those involved in glycolysis, carbohydrate metabolism and amino acid synthesis may re ect enhanced plant growth and development [47,48].…”

Section: Discussionsupporting

confidence: 90%

Comparative Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins Involved in Grain-Filling Rate of Rice at the Early Ripening Stage

Chen

Cao

Huang

et al. 2020

Preprint

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Background Grain-filling ability is a determinant factor of rice potential yield. Grain filling is a biological process of starch accumulation involved a large number of major enzymes implicated in carbohydrates metabolism in developing rice endosperm and is governed by a complex balance between the sink (grains) and the source (assimilates). This study was carried out to analyze the proteomic profile of rice grains and to identify proteins associated with high grain-filling rate during the early ripening period in rice. Results TMT and LC-MS/MS were employed in analyzing proteomic profile of grains from two rice cultivars possess contrasting phenotypes in grain filling rate to identify proteins associated with high grain-filling rate during the early ripening stage. The two cultivars differed significantly in grain-filling rate during the period of 0–3 days after full heading, indicating the appropriacy of cultivars selection for quantitative proteomic analysis. A total of 219 differentially expressed proteins in grains between the two cultivars were identified, providing a database for quantified proteomics in rice grains during grain filling stage. Elevated expression of many enzymes involved in starch and sucrose metabolism during rice grain filling was observed. GO and KEGG analyses revealed that the largest portion of differentially expressed proteins during grain filling associated with carbohydrate metabolism and transportation, suggesting a more specific function of those protein in rice grain development. Starch, sucrose, fatty acids and amino acids biosynthesis and metabolism were significantly enriched pathways. Conclusions Analysis of protein-protein interactions indicated the implication of the same proteins in several biological processes such as carbohydrate transport and metabolism, fatty acid metabolism, energy production and conversion and secondary metabolites biosynthesis. The data provide valuable information about the roles of biosynthesis, transport and metabolism of carbohydrate and amino acids in rice grain filling and development. The results represent a valuable foundation for further studies of the roles of differentially expressed proteins in underlying grain filling stage in rice and their potential impacts on rice productivity.

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Section: Discussionsupporting

confidence: 90%

Comparative Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins Involved in Grain-Filling Rate of Rice at the Early Ripening Stage

Chen

Cao

Huang

et al. 2020

Preprint

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“…Most of the N accumulation took place within 5 wk after flowering in soybean. This has management implications for efforts targeting increases in protein or specific amino acid content via nutrient amendments (7,17,18). Depending on the time required for N uptake by the growing soybean plant, time of N application can be adjusted.…”

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confidence: 99%

Genomic regions governing soybean seed nitrogen accumulation

Panthee

Pantalone

Sams

et al. 2004

J Americ Oil Chem Soc

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Nitrogen accumulation in the form of seed protein takes place in soybean [Glycine max (L.) Merr.] during the reproductive stages of development. The purpose of this study was to relate genotypic differences in seed nitrogen accumulation with genomic regions controlling nitrogen accumulation in soybean during R 5 , R 6 , and R 7 growth stages. A population of 101 F 6:8 recombinant inbred lines (RIL) developed from a cross of N87-984-16 × TN93-99 was utilized. The RIL were grown at the University of Tennessee, Knoxville Experiment Station, in a randomized complete block design with three replications in 2002. Seed nitrogen was determined from pod samples harvested at the R 5 , R 6 , and R 7 growth stages. A significant (P < 0.05) difference among genotypes was found for nitrogen accumulation at all three growth stages. Single-factor ANOVA revealed that quantitative trait loci (QTL) governing nitrogen accumulation in soybean seed were distributed in the linkage groups A2, B2, D1a, D1b, E, G, and M. Phenotypic variation explained by an individual QTL ranged from 5 to 11.6%. These QTL may provide useful markerassisted selection opportunities for soybean protein improvement.Soybean [Glycine max (L.) Merr.] seeds typically contain approximately 40% protein on a dry weight basis, making soybeans one of the highest protein-containing crop species. Nitrogen (N) is an important constituent of storage protein that is accumulated during reproductive stages of seed development. The rate of storage protein accumulation can be estimated from the rate of N accumulation.Understanding the physiology of N accumulation and protein synthesis should allow more efficient breeding to develop high-protein soybean lines. According to Shibles et al.(1), sources of N include uptake of soil nitrate during seed growth, fixation of atmospheric N 2 during seed growth, and redistribution of N accumulated in vegetative tissues before seed growth begins. Staswick et al.(2) determined that large quantities of N are also mobilized from mature organs to developing seeds. Imsande (3) found that leaf proteins are hydrolyzed and that the salvaged N is translocated to the developing pods and seeds during soybean pod filling. An increase in the protein content of the cotyledon commences as early as 15 d after flowering and continues through to maturity. The most rapid rate of accumulation of total protein in cotyledons is between 25 to 40 d after flowering (4). Accumulation of N into growing seeds and formation of seed storage proteins, which contribute to soybean seed yield, have important economic implications.Kumudini et al. (5) demonstrated a genotypic effect in seed N accumulation in soybeans, where an ancestral set of cultivars ('Pagoda' and 'Mandarin') was superior in this process compared with later cultivars ('Maple Glen' and 'OAC Bayfield'). Recently, Grandgirard (6) showed that the seed N accumulation rate in soybeans is determined by the genotype. However, no reports are available about the genomic regions or quantitative trait loci (QTL) contr...

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“…Studies in various parts of the world assessed the amino-acid composition, protein fractionation, and electrophoretic characteristics of protein, which is the main component of soybeans [ 18 , 19 , 20 ]. Studies were also conducted on the lipid component, in addition to it assessment related to the processing and storage of soybeans [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in Soybean (Glycine max L.) Flour Fatty-Acid Content Based on Storage Temperature and Duration

Prabakaran

Lee

et al. 2018

Molecules

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Soybeans are low in saturated fat and a rich source of protein, dietary fiber, and isoflavone; however, their nutritional shelf life is yet to be established. This study evaluated the change in the stability and quality of fatty acids in raw and roasted soybean flour under different storage temperatures and durations. In both types of soybean flour, the fatty-acid content was the highest in the order of linoleic acid (18-carbon chain with two double bonds; C18:2), oleic acid (C18:1), palmitic acid (C16:0), linolenic acid (18:3), and stearic acid (C18:0), which represented 47%, 26%, 12%, 9%, and 4% of the total fatty-acid content, respectively. The major unsaturated fatty acids of raw soybean flour—oleic acid, linoleic acid, and linolenic acid—decreased by 30.0%, 94.4%, and 97.7%, and 38.0%, 94.8%, and 98.0% when stored in polyethylene and polypropylene film, respectively, after 48 weeks of storage under high-temperature conditions. These values were later increased due to hydrolysis. This study presents the changes in composition and content of two soybean flour types and the changes in quality and stability of fatty acids in response to storage temperature and duration. This study shows the influence of storage conditions and temperature on the nutritional quality which is least affected by packing material.

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Differential Accumulation of Soybean Seed Storage Protein Subunits in Response to Sulfur and Nitrogen Nutritional Sources

Cited by 34 publications

References 30 publications

Comparative Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins Involved in Grain-Filling Rate of Rice at the Early Ripening Stage

Comparative Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins Involved in Grain-Filling Rate of Rice at the Early Ripening Stage

Genomic regions governing soybean seed nitrogen accumulation

Changes in Soybean (Glycine max L.) Flour Fatty-Acid Content Based on Storage Temperature and Duration

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