Differential Action of Silver Nanoparticles on ABCB1 (MDR1) and ABCC1 (MRP1) Activity in Mammalian Cell Lines

Abstract

Silver nanoparticles (AgNPs), due to their unique properties have been receiving immense attention in recent years. In addition to their antibacterial and antifungal activities, AgNPs also cause apoptosis, mitochondria disfunction, nucleic acid damage and show potent anticancer properties in both multidrug resistance (MDR) and sensitive tumors. The MDR phenomenon, caused by the presence of ATP-binding cassette (ABC) proteins, is responsible for the failure of chemotherapy. Thus, investigating the influence of widely used AgNPs on ABC transporters is crucial. In the present study, we have examined the cytotoxicity of silver nanoparticles of a nominal size of 20 nm (Ag20) on the cell lines of different tissue origins. In addition, we have checked the ATP-binding cassette transporters’ activity and expression under AgNP exposure. The results indicate that Ag20 shows a toxic effect on tested cells, as well as modulating the expression and transport activity of ABC proteins.

Identification and Validation of ATP-Binding Cassette Transporters Involved in the Detoxification of Abamectin in Rice Stem Borer, Chilo suppressalis

“…In mammals, the ABC transporters participate in the absorption and excretion of drugs and their overexpression leads to the multidrug resistance of cancer cells and prevents the accumulation of chemotherapy drugs, which might be the main reason for the failure of cancer chemotherapy. , The transport functions of human ABC transporters have been widely studied by recombinant expression in vitro. The overexpression of ABC transporters including the ABCB, ABCC, and ABCE genes in cancer cells promoted cell growth, invasion, and metastasis, while the inhibition of the drug transport activity of cancer cells by ABC transport inhibitors reduced the drug outflow. − In insects, the role of ABC transporter in the transport of xenobiotics was rarely studied in vitro. While a study found that PxABCC2 was upregulated in the insecticide-resistant strains of P.…”

The Role of Silver Nanoparticles in the Diagnosis and Treatment of Cancer: Are There Any Perspectives for the Future?

“…Furthermore, the effects of AgNPs on MDR phenotype depend not only on their molecular size, but also on the type of affected tumor cells. Despite Western blotting analysis confirming the presence of ABCB1 transporter in several cell lines of different tissue origin (human non-small-cell lung carcinoma, A549; human hepatocellular carcinoma, HepG2; and human colon adenocarcinoma, SW620), AgNPs modestly diminished ABCB1 protein expression only in A549 and SW620 cells [ 182 ]. Overall, the lowest protein expression of ABCB1 protein was noted in lung cancer cells.…”

The toxicity of nanoparticles and their interaction with cells: an in vitro metabolomic perspective

“…184,185 Regarding the use of AgNPs as potential drug carriers for cancer therapy from proteogenomic and metabolomic perspectives, the reader is directed to the review by Raja et al 186 AgNPs have been shown to influence different cells causing apoptosis, lipid peroxidation, and DNA damage. 187–190…”