Differential Activation of Immune Cells for Genetically Different Decellularized Cardiac Tissues

Methe, Ketaki; Nayakawde, Nikhil B.; Banerjee, Debashish; Sihlbom, Carina; Agbajogu, Christopher; Travnikova, Galyna; Olausson, Michael

doi:10.1089/ten.tea.2020.0055

Cited by 16 publications

(15 citation statements)

References 80 publications

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“…Most studies have mainly quantified just shifts in mast cell numbers associated with biomaterial implantation, the results of which are suspect given the general low numbers of mast cells in tissue. [78] The reader is directed to an excellent review by Ozpinar et al, [79] on the influence of various biomedical implant design factors (i.e., natural versus synthetic material of construction, topography/porosity, biologically activated or decorated) on MC responses.…”

Section: Mast Cellsmentioning

confidence: 99%

Biocompatibility Evolves: Phenomenology to Toxicology to Regeneration

Crawford

Wyatt

Bryers

et al. 2021

Adv Healthcare Materials

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The word "biocompatibility," is inconsistent with the observations of healing for so-called biocompatible biomaterials. The vast majority of the millions of medical implants in humans today, presumably "biocompatible," are walled off by a dense, avascular, crosslinked collagen capsule, hardly suggestive of life or compatibility. In contrast, one is now seeing examples of implant biomaterials that lead to a vascularized reconstruction of localized tissue, a biological reaction different from traditional biocompatible materials that generate a foreign body capsule. Both the encapsulated biomaterials and the reconstructive biomaterials qualify as "biocompatible" by present day measurements of biocompatibility. Yet, this new generation of materials would seem to heal "compatibly" with the living organism, where older biomaterials are isolated from the living organism by the dense capsule. This review/perspective article will explore this biocompatibility etymological conundrum by reviewing the history of the concepts around biocompatibility, today's standard methods for assessing biocompatibility, a contemporary view of the foreign body reaction and finally, a compendium of new biomaterials that heal without the foreign body capsule. A new definition of biocompatibility is offered here to address advances in biomaterials design leading to biomaterials that heal into the body in a facile manner.

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Section: Mast Cellsmentioning

confidence: 99%

Biocompatibility Evolves: Phenomenology to Toxicology to Regeneration

Crawford

Wyatt

Bryers

et al. 2021

Adv Healthcare Materials

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“…It is also important to note that in group (1), mast cells without signs of degranulation were found among the cells that migrated into the thickness of the membrane matrix. It is known that mast cells, as resident connective tissue cells, play a key role in the development of a foreign body chronic reaction , as well as allergic reactions, regeneration processes, angiogenesis and immune protection [17]. According to the literature analysis, mast cells also participate in the processes of biomaterial resorption, however, these processes must be accompanied by degranulation and release of inracellular mediators and proteases [17].…”

Section: Results Of Evaluation Of Biointegration Barrier Function And...mentioning

confidence: 99%

Elastin Barrier Membranes for Guided Tissue Regeneration Technologies

et al. 2023

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This article discusses the prospects for the use of new elastin barrier membranes manufactured using adapted technologies for the selective isolation of the elastin component from the extracellular xenogenic matrix of the pericardium ligamentous apparatus: (1) by high-temperature extraction under pressure; (2) cyanogen bromide method. A commercial material, Geistlich Bio–Gide® membrane (BG), was used as a control comparison group. It is shown that the materials of group (1) have a high degree of biocompatibility, exceeding the indicators of the control group BG. Based on the results of an study in a model of subcutaneous heterotopic implantation in rats, it was shown that elastin BM has a chemoattractant effect on the mesenchymal recipient cells and, unlike the control, is able to integrate to a high degree into the surrounding recipient tissues. At the same time, the materials of group (1) had a pronounced proangiogenic effect. Thus, it has been shown that elastin BM groups (1) have a medium-term barrier function and are able to induce full-fledged cellular repopulation and local neoangiogenesis, which can be useful in clinical practice, primarily in GTR technologies (with gingival flap augmentation) or when used together with other BM as an angiogenesis inducer to ensure formation of the vascular bed in GBR technologies of bone tissue.

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“…As a source of the acellular scaffold, allogenic biomaterials have certain advantages such as maintenance of native tissue composition, architecture, remodeling ability, and regenerate a host tissue while circumventing the immune responses [33] . However, the allogeneic scaffold/implant is also encountered with adverse immune responses driven by the type and genetic mismatch of scaffolds [34] . The development of an acellular scaffold by natural means could help overcome the problem associated with synthetic, biological scaffolds as well as allogeneic scaffolds owing to minimizing of unfavorable immune responses by selecting appropriate routes.…”

Section: Discussionmentioning

confidence: 99%

Differential fate of acellular vascular scaffolds in vivo involves macrophages and T-cell subsets

Banerjee

Nayakawde

Antony

et al. 2020

Preprint

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The development of a suitable biological scaffold or implant is a pre-requisite for the functional development of organs including blood vessels. Biological scaffold prepared by physical or chemical agents cause damage to the extracellular matrix architecture and induce immune response after scaffold implantation/engraftment. During the current study, taking advantage of different host tissue environments (subcutaneous vs omentum), acellular scaffolds/implants were prepared based on syngeneic vs allogeneic model of scaffold implantation (in vivo decellularization) and compared with chemically decellularized scaffolds. Employing molecular, proteomics, and histologic techniques, we confirmed that site-specific advantages exist in modulating the ECM as well as regulating the immune responses (macrophage and T-cells) to produce an acellular scaffold without the need for immunosuppressants. The current approach opens up the possibility to create tailor-made scaffolds for further use in the clinics with a potential for long term acceptability without eliciting adverse reactions in the host. The scaffolds prepared thus would open up a further possibility to build functional organs following cell engraftment.Impact statementDuring the current study an alternative strategy of preparing a scaffold by natural means was investigated which would also give an option to avoid the detrimental effect of chemicals used while preparing a biological scaffold. Understanding the cellular milieu of an implanted scaffold would help to modify the immunological trigger upon scaffold implantation, an aspect also studied during the current research. The infiltrating cells and their putative relationship with the biological scaffold could help the researcher to target immune cells to avoid scaffold/implant rejection. In a nutshell, the study carried out without any immunosuppressive agents could help further exploration of (a) alternative strategies for preparing biological scaffolds and (b) implantable sites as potential bioreactors with the aim to avoid any adverse immune reactions for further acceptance of the scaffold/implant post implantation.

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Differential Activation of Immune Cells for Genetically Different Decellularized Cardiac Tissues

Cited by 16 publications

References 80 publications

Biocompatibility Evolves: Phenomenology to Toxicology to Regeneration

Biocompatibility Evolves: Phenomenology to Toxicology to Regeneration

Elastin Barrier Membranes for Guided Tissue Regeneration Technologies

Differential fate of acellular vascular scaffolds in vivo involves macrophages and T-cell subsets

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