Differential amperometric measurement of serum lactate dehydrogenase activity using Bindschedler's green

Detection of NADH via electrocatalytic oxidation at single-walled carbon nanotubes modified with Variamine blue

“…The reason for which we investigated the response of our modified electrodes coupled with FIA [48], is based on the fact that redox mediators reported in literature [21][22][23][24][25][26][27][28][29][30] were not always suited for long term monitoring of reactions involving NADH, sometimes because of their poor stability.…”

“…Using a wide range of electron mediators, the oxidation potential of NADH is reduced to the formal redox potential of the mediator, with relatively improved efficiency regarding the electrocatalytic oxidation process of NADH. Several mediators have been reported in the literature, among them quinones [21,22], oxometalates [23], ruthenium complexes [24], quinonoid redox dyes such as indamines [25], phenazines [26,27], phenoxazines [28,29] and phenothiazines [30]. These redox mediators are very efficient as electron shuttles for NADH oxidation, but they are not always suited for long term monitoring of reactions involving NADH because of their poor stability; some of them are highly water soluble, others loose sensitivity after deposition on electrodes, or exhibit too strong pH dependency.…”

Recent advances in NADH electrochemical sensing design

“…The easier way to achieve NADH electrooxidation at low overpotentials is historically represented by the use of redox mediators [9][10][11][12][13][14][15], the working applied potential necessary for NADH oxidation being reduced to the formal redox potential of the mediator, with relatively improved efficiency of the electrocatalytic oxidation process. The proper use of such mediators, the proper electrochemical tools for their investigation, the rationale for the selection of the proper mediator as well as the kinetic and thermodynamic constraints resulting by their use for NADH sensing has been extensively reviewed by Gorton and Dominguez in 2002 [16].…”

Determination of lactate dehydrogenase in human erythrocytes by capillary electrophoresis with electrochemical detection

“…There was a report of detection of LDH by electrophoresis fluorometric method [2]. A procedure was described for determination of serum LDH activity by use of Bindschedler's Green [3]. Capillary electrophoresis (CE) with laser induce fluorescence (LIF) detection has been used to detect LDH in erythrocyte [4] and leukemia [5], even single LDH molecules [6].…”