Differential Analysis of the Nasal Microbiome of Pig Carriers or Non-Carriers of Staphylococcus aureus

Espinosa-Gongora, Carmen; Larsen, Niels; Schønning, Kristian; Fredholm, Merete; Guardabassi, Luca

doi:10.1371/journal.pone.0160331

Cited by 38 publications

(40 citation statements)

References 46 publications

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“…aureus colonization in the nose [ 49 , 50 ]. In addition to the role for S. epidermidis and Dolosigranulum species, a role for Lachnospiraceae should be explored in S. aureus non-carrier phenotype as suggested by Espinosa-Gongora et al [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

The nasal microbiota of dairy farmers is more complex than oral microbiota, reflects occupational exposure, and provides competition for staphylococci

Shukla

Sandberg

et al. 2017

PLoS ONE

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Allergic and autoimmune diseases had been attributed to lack of exposure to biodiversity, an important factor in regulating immune homeostasis in a healthy host. We posit that the microbiome of healthy dairy farmers (DF) will be richer than non-farmers (NF) living in urban settings due to exposure to a greater biodiversity in the dairy environment. However, no studies have investigated the relationships between microbiota of dairy farmers (DF) compared with urban non-farmers (NF). We compared the nasal and oral microbiota of dairy farmers (N_DF, O_DF, respectively) with nasal and oral microbiota of NF in the same geographical area. The N_DF showed high microbial diversity with hundreds of unique genera that reflected environmental/occupational exposures. The nasal and oral microbiomes clustered separately from each other using Principal Coordinate Analysis, and with DF harboring two-fold and 1.5-fold greater exclusive genera in their nose and mouth respectively, than did non-farmers. Additionally, the N_DF group had a lower burden of Staphylococcus spp. suggesting a correlation between higher microbial diversity and competition for colonization by staphylococci. The N_DF samples were negative for the mecA gene, a marker of methicillin-resistance in staphylococci. The lower burden of staphylococci was found to be independent of the abundance of Corynebacterium spp. Exposure to greater biodiversity could enhance microbial competition, thereby reducing colonization with opportunistic pathogens. Future studies will analyze whether exposure to livestock microbiomes offers protection from acute and chronic diseases.

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Section: Discussionmentioning

confidence: 99%

The nasal microbiota of dairy farmers is more complex than oral microbiota, reflects occupational exposure, and provides competition for staphylococci

Shukla

Sandberg

et al. 2017

PLoS ONE

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“…The variations in the microbiota composition associated with both types of G. parasuis strains could be driven by the physiology of the different bacterial community, which could impact the magnitude of virulent or non-virulent strains colonization and prevalence, and subsequently, the piglet's health status [4]. In a similar infection study, Espinosa-Gongora et al [13] reported that nasal microbiome of pigs that are not colonized with Staphylococcus aureus harbors several species/taxa that are significantly less abundant in carrier pig, suggesting that the nasal microbiota plays a role in the individual predisposition to S. aureus nasal carriage in pigs. In the earlier study by Correa-Fiz et al [1], a marked difference between the composition and diversity of the nasal microbiota between swine farms with different health status was reported.…”

Section: Microbiota Associated With G Parasuis Of Different Virulencementioning

confidence: 99%

“…Several recent studies uncovered the nasal microbiota composition in pigs and their association with different infectious pathogens [1,[12][13][14]. Strube et al [14] showed that the porcine nasal mucosa harbored Rothia and had higher relative levels of Streptococcus and Moraxella, while levels of Aerococcus, Facklamia and Jeotgalibaca were relatively lower.…”

Section: Introductionmentioning

confidence: 99%

“…Strube et al [14] showed that the porcine nasal mucosa harbored Rothia and had higher relative levels of Streptococcus and Moraxella, while levels of Aerococcus, Facklamia and Jeotgalibaca were relatively lower. Espinosa-Gongora et al [13] reported that the nasal microbiota may play a role in the individual predisposition to Staphylococcus aureus nasal carriage in pigs. On the contrary, Weese et al [12] found no impact of the nasal microbiota on the methicillin-resistant Staphylococcus aureus carriage in pigs.…”

Section: Introductionmentioning

confidence: 99%

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Variations in association of nasal microbiota with virulent and non-virulent strains of Glaesserella (Haemophilus) parasuis in weaning piglets

Mahmmod

Correa‐Fiz

Aragón

2020

Vet Res

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Glaesserella (formerly Haemophilus) parasuis causes Glässer's disease, which results in high economic loss in the swine industry. To understand the polymicrobial interactions of G. parasuis and the nasal microbiota, the statistical association patterns of nasal colonizing bacteria with virulent and non-virulent strains of G. parasuis were studied accounting for the farm management practices as potential risk factors for the occurrence of Glässer's disease. The nasal microbiota from 51 weaned-piglets from four farms with Glässer's disease and three farms with no respiratory diseases was previously characterized and included in this study. The presence of virulent and/or non-virulent G. parasuis strains in the nasal cavities was determined in order to establish the potential association with other members of the nasal microbiota. Multivariate logistic and linear regression models were performed among the various members of nasal microbiota and G. parasuis. The multi-site production system and disease presence in the farm were both significantly associated with the presence of G. parasuis virulent strains in the nose of the piglets. Differential bacterial associations were observed with virulent or non-virulent G. parasuis. Chitinophagaceae, Corynebacteriaceae and Corynebacterium were positively associated with the virulent G. parasuis strains, while Enterobacteriaceae, Peptostreptococcaceae, Clostridium XI, and Escherichia/Shigella were negatively associated with virulent G. parasuis. On the other hand, Flavobacteriaceae, Planobacterium, and Phascolarctobacterium were positively associated with the non-virulent G. parasuis strains, while Rikenellaceae, Enterococcaceae, Odoribacter, and Corynebacterium were negatively associated with non-virulent G. parasuis. In conclusion, the nasal microbiota communities showed variations in the association with the G. parasuis strains type.

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“…Recent studies have investigated the impact of the microbiota in S . aureus carriage in both pigs 21 and mice 22 .…”

Section: Introductionmentioning

confidence: 99%

Development of persistent gastrointestinal S. aureus carriage in mice

Flaxman

Diemen

Yamaguchi

et al. 2017

Sci Rep

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One fifth to one quarter of the human population is asymptomatically, naturally and persistently colonised by Staphylococcus aureus. Observational human studies indicate that although the whole population is intermittently exposed, some individuals lose S. aureus rapidly. Others become persistent carriers, as assessed by nasal cultures, with many individuals colonised for decades. Current animal models of S. aureus colonisation are expensive and normally require antibiotics. Importantly, these animal models have not yet contributed to our poor understanding of the dichotomy in human colonisation status. Here, we identify a single strain of S. aureus found to be persistently colonising the gastrointestinal tract of BALB/c mice. Phylogenetic analyses suggest it diverged from a human ST15 lineage in the recent past. We show that murine carriage of this organism occurs in the bowel and nares, is acquired early in life, and can persist for months. Importantly, we observe the development of persistent and non-persistent gastrointestinal carriage states in genetically identical mice. We developed a needle- and antibiotic-free model in which we readily induced S. aureus colonisation of the gastrointestinal tract experimentally by environmental exposure. Using our experimental model, impact of adaptive immunity on S. aureus colonisation could be assessed. Vaccine efficacy to eliminate colonisation could also be investigated using this model.

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Differential Analysis of the Nasal Microbiome of Pig Carriers or Non-Carriers of Staphylococcus aureus

Cited by 38 publications

References 46 publications

The nasal microbiota of dairy farmers is more complex than oral microbiota, reflects occupational exposure, and provides competition for staphylococci

The nasal microbiota of dairy farmers is more complex than oral microbiota, reflects occupational exposure, and provides competition for staphylococci

Variations in association of nasal microbiota with virulent and non-virulent strains of Glaesserella (Haemophilus) parasuis in weaning piglets

Development of persistent gastrointestinal S. aureus carriage in mice

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