Differential appearance of the subunits of glycoprotein hormones (LH, FSH, and TSH) in the pituitary of bullfrog (Rana catesbeiana) larvae during metamorphosis

Tanaka, Shigeyasu; Sakai, Makoto; Park, Min Kyun; Kurosumi, Kazumasa

doi:10.1016/0016-6480(91)90055-b

Cited by 29 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On this assumption, we employed antisera raised against mammalian, avian, and synthetic hormones to identify the hypophysial endocrine cells and hypothalamic neu- roendocrine cells and fibers in the present study. Recently, antibodies raised against anuran hormones have been histochemically applied for the identification of hypophysial cells in anurans (Gaustalla et al, 1993;Kobayashi and Kikuyama, 1991;Tanaka et al, 1990Tanaka et al, , 1991. These previous studies indicated that 1) these antibodies allow the determination of immunoreactive cells a t higher dilutions than those generated against mammalian hormones, probably due to their higher affinity; and 2) they did not alter the distribu- tion of the cells.…”

Section: Methodological Validity (General Aspects)mentioning

confidence: 97%

“…The development of endocrine cells of the hypophysis has been studied immunohistochemically and reported by many authors in numerous species of mammals and birds (Dupouy, 1980;Mikami, 1986;Osamura and Watanabe, 1985), reptiles (Licht and Pearson, 1978), several amphibians (Guastalla et al, 1993;Kar and Naik, 1986;Moriceau-Hay et al, 1982;Nyholm and DoerrSchott, 1977;Tanaka et al, 1991), and some species of teleosts (Naito et al, 1993;Power and Canario, 1992). The distribution and development of hypothalamic neuroendocrine cells and fibers is well documented in mammals (Ugrumov, 1991).…”

Section: Hypothalamus Immunohistochemistry Xenopus Zaeuismentioning

confidence: 95%

See 1 more Smart Citation

Immunohistochemical studies on the development of the hypothalamo‐hyophysial system in Xenopus laevis

1995

View full text Add to dashboard Cite

These results suggest the following three chronological steps in the development of hypothalamo-hypophysial systems and their target organs: independent development of target organs at early developmental stages; appearance of hypophysial hormones to control the development of target organs at middle developmental stages; appearance of hypothalamic hormones to control the function or maturation of the hypophysis at late developmental stages.

show abstract

Section: Methodological Validity (General Aspects)mentioning

confidence: 97%

Section: Hypothalamus Immunohistochemistry Xenopus Zaeuismentioning

confidence: 95%

Immunohistochemical studies on the development of the hypothalamo‐hyophysial system in Xenopus laevis

1995

View full text Add to dashboard Cite

show abstract

“…Au cours de cette dernière, des pics plasmatiques successifs de thyroxine, de cortisone et de prolactine (PRL) ont été mis en évidence chez Rana catesbeiana (DICKHOFF et al, 1990). Une augmentation, dans l'hypophyse, de la sous-unité a des hormones glycoprotéiques (TANAKA et al, 1991) et des ARN messagers codant pour l'hormone de croissance (TAKAHASHI et al, 1992) a également été démontrée. Chez les Téléostéens, la spectaculaire métamorphose larvaire des Pleuronectidés implique aussi les hormones thyroïdiennes et le Cortisol (DE JESUS et al, 1990).…”

Section: Les Mécanismes Neuroendocriniensunclassified

L'argenture de l'Anguille : métamorphose, anticipation, adaptation

Fontaine

1994

Bull. Fr. Pêche Piscic.

View full text Add to dashboard Cite

RÉSUMÉL'argenture est une métamorphose qui, au cours du cycle vital des anguilles, précède la migration marine de reproduction. Une meilleure connaissance de ce phénomène serait utile non seulement d'un point de vue fondamental en biologie du développement mais aussi d'un point de vue appliqué pour la gestion des populations de ce poisson.On passe d'abord en revue un certain nombre de caractères très variés, qui ont été invoqués pour différencier les stades jaune et argenté, en s'attachant à la signification, pour le cycle vital, des nouveautés exprimées durant l'argenture ; la plupart de celles-ci apparaissent être des caractères adaptatifs qui anticipent sur les conditions de la migration marine.On s'intéresse ensuite à la dynamique et au déterminisme neuroendocrinien de l'argenture. On suggère l'existence de plusieurs étapes qui se succéderaient du printemps à la fin de l'automne, de la préargenture à la descente des rivières (l'avalaison). En se référant aux rares données neuroendocrinologiques disponibles ainsi qu'à d'autres métamorphoses comme celles des amphibiens et la smoltification du saumon, on est conduit à proposer qu'un certain nombre d'hormones interviennent dans le déterminisme des étapes successives de l'argenture. Un des événements précoces pourrait être la stimulation de neurones hypothalamiques, avec sécrétion de neuropeptides hypophysiotropes. Une question particulièrement intéressante posée par l'argenture de l'anguille, et sur laquelle on insiste, est celle du couplage des déclenchements de la métamorphose et de la puberté.Un scénario évolutif est proposé pour l'apparition du cycle vital si complexe des anguilles à partir de la vie entièrement marine qui devait être celle de leurs ancêtres comme elle l'est encore pour la plupart de leurs proches parents, les autres élopomorphes. Ce scénario implique en particulier l'acquisition de l'euryhalinité, puis «l'assimilation génétique" des processus d'abord mis en oeuvre lors de l'accommodation à l'eau de mer ; c'est dire que ces processus ont dû, au cours de l'évolution, être placés sous le contrôle de l'horloge du développement qui déclenche l'argenture ; des .hypothèses neuroendocriniennes sont suggérées pour expliquer cette assimilation génétique.En conclusion, on s'interroge sur la définition morphologique et biochimique des étapes successives de l'argenture et sur la reconnaissance de ces étapes par des caractères, aisément détectables, plus précis et fiables que la coloration. Des progrès dans ces domaines permettraient de connaître par exemple, dès avant l'avalaison, la proportion du stock qui quittera les eaux douces dans l'année.Mots-clés : anguille, métamorphose, migration, physiologie, évolution. EEL SILVERING : METAMORPHOSIS, ANTICIPATION, ADAPTATION. SUMMARYEel "silvering" is a metamorphosis which, during the life cycle of these fish, precedes the marine reproductive migration. A better knowledge of this phenomenon would be useful not only from a fundamental point of view in developmental biology but also from an applied point of vie...

show abstract

“…The resulting media (150 l) were collected in V-bottom 96-well microplates (Iwaki, Tokyo, Japan) following centrifugation at 100 ϫ g for 5 min to remove cell debris. The collected medium (100 l) was stored at Ϫ20°C until use in a homologous radioimmunoassay for GH (22), PRL (23), FSH (24), and LH (25).…”

Section: Methodsmentioning

confidence: 99%

Bullfrog Ghrelin Is Modified by n-Octanoic Acid at Its Third Threonine Residue

Kaiya¹,

Kojima²,

Hosoda³

et al. 2001

Journal of Biological Chemistry

155

101

View full text Add to dashboard Cite

We have identified the amphibian ghrelin from the stomach of the bullfrog. We also examined growth hormone (GH)-releasing activity of this novel peptide in both the rat and bullfrog. The three forms of ghrelin identified, each comprised of 27 or 28 amino acids, possessed 29% sequence identity to the mammalian ghrelins. A unique threonine at amino acid position 3 (Thr 3 ) in bullfrog ghrelin differs from the serine present in the mammalian ghrelins; this Thr 3 is acylated by either n-octanoic or n-decanoic acid. The frog ghrelin-28 has a complete structure of GLT (O-n-octanoyl)FLSPAD-MQKIAERQSQNKLRHGNM; the structure of frog ghrelin-27 was determined to be GLT(O-n-octanoyl)FLSPAD-MQKIAERQSQNKLRHGN; frog ghelin-27-C10 possessed a structure of GLT(O-n-decanoyl)FLSPADMQKIAER-QSQNKLRHGN. Northern blot analysis demonstrated that ghrelin mRNA is predominantly expressed in the stomach. Low levels of gene expression were observed in the heart, lung, small intestine, gall bladder, pancreas, and testes, as revealed by reverse transcription polymerase chain reaction analysis. Bullfrog ghrelin stimulated the secretion of both GH and prolactin in dispersed bullfrog pituitary cells with potency 2-3 orders of magnitude greater than that of rat ghrelin. Bullfrog ghrelin, however, was only minimally effective in elevating plasma GH levels following intravenous injection into rats. These results indicate that although the regulatory mechanism of ghrelin to induce GH secretion is evolutionary conserved, the structural changes in the different ghrelins result in species-specific receptor binding. Growth hormone (GH)1 secretion from the pituitary gland is regulated by hypothalamic hormones; growth hormone-releasing hormone (GHRH) stimulates GH secretion, whereas somatostatin is inhibitory (1). Derivatives of Met-enkephalin stimulate GH release (2), the first demonstration that small synthetic peptides and nonpeptide molecules, dubbed growth hormone secretagogues (GHSs), can mediate GH release through a receptor distinct from that of GHRH (3-5). The G-protein-coupled GHS receptor (GHS-R) was subsequently identified in swine, rat, and human (6, 7), suggesting that one or more unknown ligands for this receptor are endogenously present.We recently discovered an endogenous ligand for GHS-R from the rat stomach, using an intracellular calcium influx assay in stable cell lines expressing rat GHS-R (8). This novel molecule, a 28-amino acid peptide named ghrelin (from "ghre," the Proto-Indo-European root of "grow"), possesses a unique serine residue at the third N-terminal position (Ser 3 ) that is n-octanoylated (8, 9). Acylation of Ser 3 is essential for ghrelin bioactivity. cDNA analysis revealed that the rat ghrelin sequence follows the 23-residue signal sequence within the 117-residue prepro-ghrelin. Ghrelin stimulates GH secretion both in vivo and in vitro. Accumulating evidence in mammals suggests that, in addition to regulating GH release, ghrelin also influences feeding behavior (10, 11), gastrointestinal function (12, 13), and ener...

show abstract

Differential appearance of the subunits of glycoprotein hormones (LH, FSH, and TSH) in the pituitary of bullfrog (Rana catesbeiana) larvae during metamorphosis

Cited by 29 publications

References 21 publications

Immunohistochemical studies on the development of the hypothalamo‐hyophysial system in Xenopus laevis

Immunohistochemical studies on the development of the hypothalamo‐hyophysial system in Xenopus laevis

L'argenture de l'Anguille : métamorphose, anticipation, adaptation

Bullfrog Ghrelin Is Modified by n-Octanoic Acid at Its Third Threonine Residue

Contact Info

Product

Resources

About