Differential Chemokine Signature between Human Preadipocytes and Adipocytes

Ignacio, Rosa Mistica C.; Gibbs, Carla; Lee, Eun Sook; Son, Deok Soo

doi:10.4110/in.2016.16.3.189

Cited by 27 publications

(26 citation statements)

References 23 publications

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“…To determine whether allogenic MSCs actively attracted T cells, we first assessed the expression profiles of chemokines and chemokine receptors that are likely to mediate T cell migration2728. We found that MSCs expressed mRNAs for CCL2, CCL3, CCL4, CXCL10, and CXCL12, and CCL2 and CXCL12 proteins were detectable by ELISA in conditioned supernatants (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

CCL2 deficient mesenchymal stem cells fail to establish long-lasting contact with T cells and no longer ameliorate lupus symptoms

Lee

Kim

et al. 2017

Sci Rep

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Systemic lupus erythematosus (SLE) is a multi-organ autoimmune disease characterized by autoantibody production. Mesenchymal stem cells (MSCs) ameliorate SLE symptoms by targeting T cells, whereas the mechanisms of their efficacy remain incompletely understood. In this study, we show that transfer of human MSCs increased MRL.Faslpr mouse survival, decreased T cell infiltration in the kidneys, and reduced T cell cytokine expression. In vitro, allogeneic mouse MSCs inhibited MRL.Faslpr T cell proliferation and cytokine production. Time-lapse imaging revealed that MSCs recruited MRL.Faslpr T cells establishing long-lasting cellular contacts by enhancing T cell VCAM-1 expression in a CCL2-dependent manner. In contrast, CCL2 deficient MSCs did not induce T cell migration and VCAM-1 expression, resulting in insufficient cell-cell contact. Consequently, CCL2 deficient MSCs did not inhibit IFN-γ production by T cells and upon transfer no longer prolonged survival of MRL.Faslpr mice. Taken together, our imaging study demonstrates that CCL2 enables the prolonged MSC–T cell interactions needed for sufficient suppression of autoreactive T cells and helps to understand how MSCs ameliorate symptoms in lupus-prone MRL.Faslpr mice.

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Section: Resultsmentioning

confidence: 99%

CCL2 deficient mesenchymal stem cells fail to establish long-lasting contact with T cells and no longer ameliorate lupus symptoms

Lee

Kim

et al. 2017

Sci Rep

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“…CCL18 is associated with some human cancer types including ovarian cancer 31 , 32 . CCL23 plays a role in subclinical systemic inflammation and associated with atherogenesis 33 . CCL8 is strengthened in stromal fibroblasts at the tumor border and in tissues at which breast cancer cells incline to metastasize such as the lungs and the brain 34 , 35 .…”

Section: Resultsmentioning

confidence: 99%

Driver pattern identification over the gene co-expression of drug response in ovarian cancer by integrating high throughput genomics data

Liao

et al. 2017

Sci Rep

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Multiple types of high throughput genomics data create a potential opportunity to identify driver patterns in ovarian cancer, which will acquire some novel and clinical biomarkers for appropriate diagnosis and treatment to cancer patients. To identify candidate driver genes and the corresponding driving patterns for resistant and sensitive tumors from the heterogeneous data, we combined gene co-expression modules with mutation modulators and proposed the method to identify driver patterns. Firstly, co-expression network analysis is applied to explore gene modules for gene expression profiles through weighted correlation network analysis (WGCNA). Secondly, mutation matrix is generated by integrating the CNV data and somatic mutation data, and a mutation network is constructed from the mutation matrix. Thirdly, candidate modulators are selected from significant genes by clustering vertexs of the mutation network. Finally, a regression tree model is utilized for module network learning, in which the obtained gene modules and candidate modulators are trained for the driving pattern identification and modulators regulatory exploration. Many identified candidate modulators are known to be involved in biological meaningful processes associated with ovarian cancer, such as CCL11, CCL16, CCL18, CCL23, CCL8, CCL5, APOB, BRCA1, SLC18A1, FGF22, GADD45B, GNA15, GNA11, and so on.

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“…Interestingly, treatment with the lipid-lowering agent atorvastatin in a randomized controlled trial reduced circulating levels of eotaxin and mRNA expression of its chemokine receptor CCR5 in peripheral CD14(+) monocytes (28). Levels of MCP1 and eotaxin were also found to be predominantly expressed in pre-adipocytes compared with adipocytes, suggesting a role of these chemokines in adipogenesis (29). In our study, there was a positive correlation between the circulating levels of MCP1 and eotaxin; however, while no change in circulating levels of eotaxin was observed in children with obesity, a significant decrease in eotaxin levels was found in the IR patients.…”

Section: Discussionmentioning

confidence: 99%

A novel approach to childhood obesity: circulating chemokines and growth factors as biomarkers of insulin resistance

et al. 2018

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Summary Background Insulin resistance (IR) in children with obesity constitutes a risk factor that should be precisely diagnosed to prevent further comorbidities. Objective Chemokines were evaluated to identify novel predictors of IR with clinical application. Methods We analysed the levels of cytokines (tumour necrosis factor [TNF] α and interleukins [ILs] 1β, 4, 6 and 10), chemokines (stromal cell derived factor 1α, monocyte chemoattract protein [MCP] 1, eotaxin and fractalkine) and growth factors (brain‐derived neurotrophic factor, pro‐fibrotic platelet‐derived growth factor [PDGF‐BB] and insulin‐like growth factor 1) in serum of prepubertal children with obesity (61 girls/59 boys, 50% IR and 50% non‐IR) and 32 controls. Factor analysis, correlation, binary logistic regression and receiver operating characteristic analysis of combined biomarkers were used to validate their capability for preventive interventions of IR. Results Changes in MCP1, eotaxin, IL1β and PDGF‐BB were observed in IR children with obesity. Bivariate correlation between stromal cell derived factor 1α, MCP1, eotaxin, TNFα, brain‐derived neurotrophic factor and/or PDGF‐BB explained the high variance (65.9%) defined by three components related to inflammation and growth that contribute towards IR. The combination of leptin, triglyceride/high‐density lipoprotein, insulin‐like growth factor 1, TNFα, MCP1 and PDGF‐BB showed a sensitivity and specificity of 93.2% for the identification of IR. The percentage of correct predictions was 89.6. Conclusions Combined set of cytokines, adipokines and chemokines constitutes a model that predicts IR, suggesting a potential application in clinical practice as biomarkers to identify children with obesity and hyperinsulinaemia.

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Differential Chemokine Signature between Human Preadipocytes and Adipocytes

Cited by 27 publications

References 23 publications

CCL2 deficient mesenchymal stem cells fail to establish long-lasting contact with T cells and no longer ameliorate lupus symptoms

CCL2 deficient mesenchymal stem cells fail to establish long-lasting contact with T cells and no longer ameliorate lupus symptoms

Driver pattern identification over the gene co-expression of drug response in ovarian cancer by integrating high throughput genomics data

A novel approach to childhood obesity: circulating chemokines and growth factors as biomarkers of insulin resistance

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