Differential Cl-and HCO3-mediated anion secretion by different colonic cell types in response to tetromethylpyrazine

Zhu, Jinxia; Yang, Ning; He, Qiong; Tsang, Lai-Ling; Zhao, Wenchao; Chung, Yiu-Wa; Chan, Hsiao-Chang

doi:10.3748/wjg.v10.i12.1763

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…Generally, in mammals including humans and rodents, PTH enhances the intestinal Ca 2ϩ absorption indirectly by stimulating renal synthesis of 1,25(OH) 2 D 3 (54). Since PTH increased the intracellular cAMP levels in most target cells (35), and the cAMP-regulated ion transporters (e.g., CFTR, NHE1, NHE3, NBCe1, and Na ϩ -K ϩ -ATPase) were abundant in mammalian intestinal cells (30,51,64), it was possible that PTH could directly activate these transporters, leading to ion transport across mammalian intestinal epithelium.…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO₃⁻ secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Laohapitakworn

Thongbunchoo

Nakkrasae

et al. 2011

American Journal of Physiology-Cell Physiology

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Section: Discussionmentioning

confidence: 99%

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO₃⁻ secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Laohapitakworn

Thongbunchoo

Nakkrasae

et al. 2011

American Journal of Physiology-Cell Physiology

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“…20) Luminal Cl Ϫ transport is predominantly via CFTR, which plays a crucial role in colonic ion transport. CFTR can also mediate cAMP-dependent HCO 3 Ϫ secretion, 21,22) which is tightly associated with Cl Ϫ secretion in rat colonic crypt. 23) As mentioned above, serosal Na…”

Section: Discussionmentioning

confidence: 99%

Appearance of Segmental Discrepancy of Anion Transport in Rat Distal Colon

Xue

Tian

Yan³

et al. 2007

Biological & Pharmaceutical Bulletin

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Anion secretion in mammalian distal colon plays an important role in body electrolyte and water balance under physiological and pathophysiological conditions. Electrogenic Cl Ϫ secretion is the fundamental means of intestinal mucosal surface hydration as a primitive defense mechanism which can prevent the mucosa from noxious stimuli, and several human diseases including secretory diarrhea and cystic fibrosis result from its defective regulation.2) HCO 3 Ϫ , similar to Cl Ϫ , is not only an important component of normal colonic fluid, contributing to the maintenance and regulation of the pH microclimate observed at the luminal surface of the colon, but also a major participator of the secreted fluid in a couple of diarrhea diseases.3) Moreover, HCO 3 Ϫ in feces is significantly reduced in patients with active ulcerative colitis, possibly due to the mucosa inflammation-induced impairment of the colonic anion exchange. 4) It has been reported that marked segmental heterogeneity of electrogenic Na ϩ transport or amiloride-sensive I SC (I SCamil) exists in human colon 5) and rat colon, 6) but few studies are concerned on the segmental difference of anion secretion within the distal colon. We previously have reported that the segmental heterogeneity of ion transport evoked by different stimulants in quantity such as 5-HT, ACh and forskolin existed in the rat distal colon, 7) but the ion components and the possible underlying mechanism were not clear. The aim of the present study is to further investigate the distinct effects of cAMP-dependent ion transport in different segments of rat distal colon, especially focus on the effect of luminal addition of adenylate cyclase activator, forskolin, since many biological or pathologic agents of alimentary tract cause gastrointestinal disorders by affecting the luminal environment of gastrointestinal, and also a very distal part of the rat colon is the part many colonic diseases predominated and easy to be affected or attacked under stress. 8) MATERIALS AND METHODS Preparation of Colonic MucosaAnimal protocols followed guidelines established by the NIH and were approved by Animal Care and Use Committee, Capital Medical University. Adult male Sprague-Dawley rats (Laboratory Animal Services Center, Capital Medical University) ranging in weight from 200 to 300 g fed with a standard rat diet and tap water, were killed by cervical dislocation, and a segment of distal colon of approximately ca. 7 cm in length was removed, rinsed with ice-cold Krebs-Henseleit solution (KHS). The distal colon was defined as the 7-cm-long segment proximal to the lymph node, (typically situated 3 cm away from the anus) as reported elsewhere.9,10) The distal colon was split into four segments termed DC 1 (next to lymph node), DC 2 , DC 3 , and DC 4 , 7) respectively. Because the effect of forskolin on the segment around lymph node (DC 1 ) is very different from the other three segments and also the variation from DC 2 to DC 4 is gradually prominent, the DC 1 and DC 4 were therefore chosen to investigate t...

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“…A recent report shows that HCO 3 − and Cl − share a secretary pathway. Because a large quantity of HCO 3 − secretes from the ileum and colonic epithelial cells (Cuthbert et al , 1999; Zhu et al , 2004), this ion appears to have an important bearing on secretory diarrhea. We speculate that HCO 3 − secretion via CFTR induces water flow from the colonocyte (Lucas, 2001).…”

Section: Resultsmentioning

confidence: 99%

Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3â

Takahashi¹,

Nakano²,

Okamoto³

et al. 2006

FEMS Microbiology Letters

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Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

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Differential Cl^-and HCO₃^-mediated anion secretion by different colonic cell types in response to tetromethylpyrazine

Abstract: AIM:Colonic epithelium is known to secrete both Cl

Cited by 9 publications

References 33 publications

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO₃⁻ secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO₃⁻ secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Appearance of Segmental Discrepancy of Anion Transport in Rat Distal Colon

Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3â

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Differential Cl-and HCO3-mediated anion secretion by different colonic cell types in response to tetromethylpyrazine

Abstract: AIM:Colonic epithelium is known to secrete both Cl

Cited by 9 publications

References 33 publications

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO3− secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO3− secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Appearance of Segmental Discrepancy of Anion Transport in Rat Distal Colon

Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3â

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Product

Resources

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Differential Cl^-and HCO₃^-mediated anion secretion by different colonic cell types in response to tetromethylpyrazine

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO₃⁻ secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Parathyroid hormone (PTH) rapidly enhances CFTR-mediated HCO₃⁻ secretion in intestinal epithelium-like Caco-2 monolayer: a novel ion regulatory action of PTH

Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3â