Differential control of cell death and gene expression during two distinct phases of hormonally-regulated muscle death in the tobacco hawkmoth Manduca sexta

Myer, Anita; Mason, Heather A.; Smith, Wendy A.; Brown, Christine; Schwartz, Lawrence M.

doi:10.1016/j.jinsphys.2008.12.006

Cited by 2 publications

(3 citation statements)

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“…(2007), indeed, reported that calreticulin acts as a marker for phagocytosis of apoptotic cells by Drosophila phagocytes. In addition, it has been known that apoptosis occurs in nerve and muscle cells during the pupal–adult metamorphic transitions in Manduca sexta (Hoffman & Weeks 2001; Myer et al . 2009).…”

Section: Resultsmentioning

confidence: 99%

“…Kuraishi et al (2007), indeed, reported that calreticulin acts as a marker for phagocytosis of apoptotic cells by Drosophila phagocytes. In addition, it has been known that apoptosis occurs in nerve and muscle cells during the pupal-adult metamorphic transitions in Manduca sexta (Hoffman & Weeks 2001;Myer et al 2009). However, the biological significance and precise functions of the induced PxCRT in pupal stages remains to be further examined with the immunological and molecular approaches using PxCRT specific antibody and RNA interference (RNAi).…”

Section: Rt-pcr Was Carried Out To Analyze Expression Patterns Ofmentioning

confidence: 99%

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Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Kim

Noh

et al. 2010

Entomological Research

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Calreticulin (CRT) plays pivotal roles in Ca2+ homeostasis, molecular chaperoning, infection, inflammation and innate immunity. In an attempt to study the involvement of CRT in innate immunity, the full‐length cDNA of calreticulin (PxCRT) was cloned from the diamondback moth, Plutella xylostella. It consists of 1674 bp (excluding poly‐A tail) with a longest open reading frame (ORF) of 1197 bp encoding 398 amino acids. In silico analysis of PxCRT ORF reveals that it has various repeat motifs and endoplasmic reticulum retention signal found in all the calreticulin proteins. As expected, high amino acid sequence identities were found from other CRTs identified from Bombyx mori (87%), Galleria mellonella (87%), Apis mellifera (74%), Anopheles gambiae (74%), Tribolium castaneum (73%), Culex quinquefasciatus (73%), Rhodnius prolixus (72%), Nasonia vitripennis (71%), Drosophila melanogaster (71%) and Haemaphysalis qinghaiensis (68%). During development, P. xylostella expressed PxCRT predominantly in the pupal stage. In addition, spatial expression pattern analysis indicates that PxCRT was highly expressed in the silk gland. PxCRT mRNA, furthermore, was strongly induced 3 to 6 h after laminarin treatment, suggesting that PxCRT appears to be involved in immune responses and also plays an important role in the silk gland.

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Section: Resultsmentioning

confidence: 99%

Section: Rt-pcr Was Carried Out To Analyze Expression Patterns Ofmentioning

confidence: 99%

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Kim

Noh

et al. 2010

Entomological Research

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“…Shortly after pupation, in the first two anterior and first two posterior segments, muscle fibres with low levels of expression of both EcR-A and B1 undergo cell death. In response to decreased ecdysone level in the four middle segments apoptosis is induced in muscle fibres with only EcR-A just before pupal ecdysis [147,148]. Despite the temporal and spatial differences, once cell death is initiated, similar molecular mechanisms are used in Manduca and Drosophila [147].…”

Section: Musclesmentioning

confidence: 99%

Ecdysone controlled cell and tissue deletion

Jiang

Denton

et al. 2019

Cell Death Differ

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The removal of superfluous and unwanted cells is a critical part of animal development. In insects the steroid hormone ecdysone, the focus of this review, is an essential regulator of developmental transitions, including molting and metamorphosis. Like other steroid hormones, ecdysone works via nuclear hormone receptors to direct spatial and temporal regulation of gene transcription including genes required for cell death. During insect metamorphosis, pulses of ecdysone orchestrate the deletion of obsolete larval tissues, including the larval salivary glands and the midgut. In this review we discuss the molecular machinery and mechanisms of ecdysone-dependent cell and tissue removal, with a focus on studies in Drosophila and Lepidopteran insects. Facts • Ecdysone is a key developmental regulator in holometabolous insects that triggers the degradation and remodeling of larval tissues during metamorphosis. • Ecdysone-mediated larval tissue deletion is spatiotemporally regulated and involves both apoptotic and autophagy-dependent cell deaths. • Ecdysone mediates its regulatory effects via a nuclear hormone receptor complex that drives the expression of target genes directly or through ecdysone-induced transcription factors.

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Differential control of cell death and gene expression during two distinct phases of hormonally-regulated muscle death in the tobacco hawkmoth Manduca sexta

Cited by 2 publications

References 48 publications

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Ecdysone controlled cell and tissue deletion

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