Differential Count and Quantitative Estimation of Granulocytes, Mononuclear Leukocytes and Renal Epithelial Cells in Urine

1979

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ABSTRACT. Rabbits were immunized with human kidney homogenate. The rabbit sera, after absorption with human liver homogenate, showed antibody activity against human kidney, renal pelvis and urinary bladder. The sera were then absorbed with human renal pelvis and urinary bladder mucosa and subsequently showed no antibody activity against urinary bladder or renal pelvis. Immunofluorescence examinations showed fluorescing cells in kidney tubules, but not elsewhere. The finally absorbed antihuman kidney sera were used for indirect immunofluorescence examination of urinary sediments from patients with renal disease. Phase contrast microscopy was used simultaneously. Fluorescence was found in cells which in phase contrast microscopy were judged to be renal tubular cells. Fluorescing cells were often found in casts, but free cells were also seen. Immunofluorescence may thus provide a means of identifying renal tubular cells in urine.

Identification of Renal Tubular Epithelial Cells in Urine with Immunofluorescence

Holm

1979

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Renal Function and Morphology in Long‐Term Lithium and Combined Lithium‐Neuroleptic Treatment

Bucht

Wentzel

et al. 1980

Ten patients on long-term lithium therapy and ten on lithium and neuroleptics (combination therapy) were examined with renal biopsy and tests of renal function. Patients on combination therapy had more pronounced histopathological changes and lower concentrating capacity than patients on lithium alone. Patients on combination therapy had received a larger total dose of lithium and had had higher maximum serum lithium levels than patients on lithium alone. Patients with large daily urine volumes had a low concentrating capacity. There was a negative correlation between degree of histopathological lesions and urinary concentrating capacity. Estimation of urinary concentrating capacity seems to be of value for the assessment of renal lesions in lithium treatment. K e y Mmds: lithium, neuroleptic therapy, urinary concentrating capacity, antidiuretic hormone, kidney pathology. Acta Med Scand 208: 381. 1980.

Urine Microscopy as Screening Method for Bacteriuria

Holm

Wahlqvist

et al. 1982

ABSTRACT. Fresh urine samples from 90 ambulatory patients with urological disorders were examined for leucocytes by counting the number of WBC/mm3 and per high power field (HPF) in sediment. Bacteria could be cultured in 20 samples, 18 of which contained >8 WBC/mm3. Two (3%) of the 63 samples with ±8 WBC/mm3 contained bacteria. Bacteriuria was found in two (3%) of 65 samples containing 0–1 WBC/HPF. Microscopic examination of the sediment might be used for the screening of patients with a high frequency and urgency of micturition for bacteriuria. A normal number of urinary leucocytes (±8/mm3) indicates that no bacteria will be found in culture. Leucocyturia indicates a high probability of bacteriuria.