Differential Development of Umbilical Cord-Derived Mesenchymal Stem Cells During Long-Term Maintenance in Fetal Bovine Serum-Supplemented Medium and Xeno- and Serum-Free Culture Medium

Le, Hang M.; Nguyen, Lung Tien; Hoang, Diem Huong; Bach, Trung Q.; Nguyen, Ha Thi Ngoc; Thi, Hien; Trinh, Dong Phuong; Nguyen, Tu Dinh; Nguyen, Liem Thanh; Than, Uyen Thi Trang

doi:10.1089/cell.2021.0050

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…We found that both hUCPVCs and BMMSCs cultured in StemMACS™ media proliferated as well as their SCM cultured counterparts. This is consistent with the findings of other groups that showed umbilical cord MSCs [ 43 – 48 ] and BMMSCs [ 49 ] exhibit similar or improved proliferation in serum- and xeno-free conditions over SCM. We also demonstrated that serum-free culture conferred greater clonogenic potential to hUCPVCs over SCM, which is consistent with previous studies on WJ MSCs in serum-free and serum-containing conditions [ 43 – 48 ].…”

Section: Discussionsupporting

confidence: 92%

“…This is consistent with the findings of other groups that showed umbilical cord MSCs [ 43 – 48 ] and BMMSCs [ 49 ] exhibit similar or improved proliferation in serum- and xeno-free conditions over SCM. We also demonstrated that serum-free culture conferred greater clonogenic potential to hUCPVCs over SCM, which is consistent with previous studies on WJ MSCs in serum-free and serum-containing conditions [ 43 – 48 ]. Two notable exceptions to our findings and those of previous studies come from Wang et al [ 50 ] and Chen et al [ 51 ], wherein the proliferative capacity of umbilical cord MSCs was greater in SCM.…”

Section: Discussionsupporting

confidence: 92%

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Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

et al. 2023

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Background Constructs currently used to repair or replace congenitally diseased pediatric heart valves lack a viable cell population capable of functional adaptation in situ, necessitating repeated surgical intervention. Heart valve tissue engineering (HVTE) can address these limitations by producing functional living tissue in vitro that holds the potential for somatic growth and remodelling upon implantation. However, clinical translation of HVTE strategies requires an appropriate source of autologous cells that can be non-invasively harvested from mesenchymal stem cell (MSC)-rich tissues and cultured under serum- and xeno-free conditions. To this end, we evaluated human umbilical cord perivascular cells (hUCPVCs) as a promising cell source for in vitro production of engineered heart valve tissue. Methods The proliferative, clonogenic, multilineage differentiation, and extracellular matrix (ECM) synthesis capacities of hUCPVCs were evaluated in a commercial serum- and xeno-free culture medium (StemMACS™) on tissue culture polystyrene and benchmarked to adult bone marrow-derived MSCs (BMMSCs). Additionally, the ECM synthesis potential of hUCPVCs was evaluated when cultured on polycarbonate polyurethane anisotropic electrospun scaffolds, a representative biomaterial for in vitro HVTE. Results hUCPVCs had greater proliferative and clonogenic potential than BMMSCs in StemMACS™ (p < 0.05), without differentiation to osteogenic and adipogenic phenotypes associated with valve pathology. Furthermore, hUCPVCs cultured with StemMACS™ on tissue culture plastic for 14 days synthesized significantly more total collagen, elastin, and sulphated glycosaminoglycans (p < 0.05), the ECM constituents of the native valve, than BMMSCs. Finally, hUCPVCs retained their ECM synthesizing capacity after 14 and 21 days in culture on anisotropic electrospun scaffolds. Conclusion Overall, our findings establish an in vitro culture platform that uses hUCPVCs as a readily-available and non-invasively sourced autologous cell population and a commercial serum- and xeno-free culture medium to increase the translational potential of future pediatric HVTE strategies. Graphical Abstract This study evaluated the proliferative, differentiation and extracellular matrix (ECM) synthesis capacities of human umbilical cord perivascular cells (hUCPVCs) when cultured in serum- and xeno-free media (SFM) against conventionally used bone marrow-derived MSCs (BMMSCs) and serum-containing media (SCM). Our findings support the use of hUCPVCs and SFM for in vitro heart valve tissue engineering (HVTE) of autologous pediatric valve tissue. Figure created with BioRender.com.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

et al. 2023

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“…We found that both hUCPVCs and BMMSCs cultured in StemMACS™ media proliferated as well as their SCM cultured counterparts. This is consistent with the ndings of other groups that showed umbilical cord MSCs (40)(41)(42)(43)(44)(45) and BMMSCs (46) exhibit similar or improved proliferation in serum-and xeno-free conditions over SCM. We also demonstrated that serum-free culture conferred greater clonogenic potential to hUCPVCs over SCM, which is consistent with previous studies on WJ MSCs in serum-free and serum-containing conditions (40)(41)(42)(43)(44)(45).…”

Section: Discussionsupporting

confidence: 92%

“…This is consistent with the ndings of other groups that showed umbilical cord MSCs (40)(41)(42)(43)(44)(45) and BMMSCs (46) exhibit similar or improved proliferation in serum-and xeno-free conditions over SCM. We also demonstrated that serum-free culture conferred greater clonogenic potential to hUCPVCs over SCM, which is consistent with previous studies on WJ MSCs in serum-free and serum-containing conditions (40)(41)(42)(43)(44)(45). Two notable exceptions to our ndings and those of previous studies come from Wang et A direct comparison of the two cell populations in each culture condition showed that hUCPVCs proliferated more than their BMMSC counterparts, as evidenced by signi cantly higher cell counts and shorter population doubling times.…”

Section: Discussionsupporting

confidence: 92%

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

Nejad

Lecce

Mirani

et al. 2023

Preprint

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Background: Constructs currently used to repair or replace congenitally diseased pediatric heart valves lack a viable cell population capable of functional adaptation in situ, necessitating repeated surgical intervention. Heart valve tissue engineering (HVTE) can address these limitations by producing functional living tissue in vitro that holds the potential for somatic growth and remodelling upon implantation. However, clinical translation of HVTE strategies requires an appropriate source of autologous cells that can be non-invasively harvested from MSC-rich tissues and cultured under serum- and xeno-free conditions. To this end, we evaluated human umbilical cord perivascular cells (hUCPVCs) as a promising cell source for in vitro production of engineered heart valve tissue. Methods:The proliferative, clonogenic, multilineage differentiation, and extracellular matrix (ECM) synthesis capacities of hUCPVCs were evaluated in a commercial serum- and xeno-free culture medium (StemMACS™) on tissue culture polystyrene and benchmarked to adult bone marrow-derived MSCs (BMMSCs). Additionally, the ECM synthesis potential of hUCPVCs was evaluated when cultured on polycarbonate polyurethane anisotropic electrospun scaffolds, a representative biomaterial for in vitro HVTE. Results:hUCPVCs had greater proliferative and clonogenic potential than BMMSCs in StemMACS™ (p<0.05), without differentiation to osteogenic and adipogenic phenotypes associated with valve pathology. Furthermore, hUCPVCs cultured with StemMACS™ on tissue culture plastic for 14 days synthesized significantly more total collagen, elastin, and sulfated proteoglycans (p<0.05), the ECM constituents of the native valve, than BMMSCs. Finally, hUCPVCs retained their ECM synthesizing capacity after 14 and 21 days in culture on anisotropic electrospun scaffolds. Conclusion:Overall, our findings establish an in vitro culture platform that uses hUCPVCs as a readily-available and non-invasively sourced autologous cell population and a commercial serum- and xeno-free culture medium to increase the translational potential of future pediatric HVTE strategies.

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“…The authors, therefore, concluded that SM medium was more suitable than PS for the expansion of therapeutic AD‐MSCs. In addition, compared with traditional fetal bovine serum‐supplemented medium, SM was found to be more suitable for UC‐MSCs amplifications, especially for practical application 91 . By comparing four MSC amplification media (SM, MesenCult, StemPro, and NutriStem), it was found that human umbilical cord blood‐derived MSCs (UCB‐MSCs) using SM could show higher cell proliferation rate, higher expression of MSC positive markers, and better triple differentiation potential and chromosome stability in the absence of serum or other animal‐derived components 92 …”

Section: Influencing Factorsmentioning

confidence: 99%

Immunogenicity of mesenchymal stromal/stem cells

et al. 2023

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Mesenchymal stromal/stem cells (MSCs) possess the ability to self‐renew and differentiate into other cell types. Because of their anti‐inflammatory and immunomodulatory abilities, as well as their more ready availability compared to other stem cell sources, MSCs hold great promise for the treatment of many diseases, such as haematological defects, acute respiratory distress syndrome, autoimmunity, cardiovascular diseases, etc. However, immune rejection remains an important problem. MSCs are considered to have low immunogenicity, but they do not have full immunological privilege. This review analyzes and discusses the safety of MSCs from the perspective of their immunogenicity, with the aim of providing a reference for future research and clinical application.

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Differential Development of Umbilical Cord-Derived Mesenchymal Stem Cells During Long-Term Maintenance in Fetal Bovine Serum-Supplemented Medium and Xeno- and Serum-Free Culture Medium

Cited by 5 publications

References 32 publications

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

Immunogenicity of mesenchymal stromal/stem cells

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