Differential effect of components of the extracellular matrix on differentiation and apoptosis

Aharoni, Dorit; Meiri, Iris; Atzmon, Ruth; Vlodavsky, Israël; Amsterdam, Abraham

doi:10.1016/s0960-9822(06)00026-1

Cited by 97 publications

(76 citation statements)

References 53 publications

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“…It is well established that the loss of cell attachment to ECM causes apoptosis, according to a process named anoikis (Ruoslahti & Reed 1994, Giancotti & Ruoslahti 1999. The present result is consistent with previous data showing that LN enhances cell survival in rat (Aharoni et al 1996) and ovine GC (Huet et al 2001). In vitro, histological observations have established that GC adjacent to the follicular basement membrane are protected from entering apoptosis in early atretic follicles (Monniaux et al 1999).…”

Section: Discussionsupporting

confidence: 82%

“…In vitro, purified LN used as a culture substratum improves GC survival in rat (Aharoni et al 1996) and sheep (Huet et al 2001) and stimulates their proliferation in sheep (Huet et al 2001). However, the effects of LN on GC steroidogenesis are still the object of controversy since LN enhances P4 secretion in rat (Aten et al 1995, Aharoni et al 1996 and pig (Sites et al 1996), but inhibits it in human . Moreover, the functional importance of LN in GC differentiation to an E2-secreting cell type during terminal follicular development, and then to a P4-secreting cell type during the luteinization process has not been established.…”

Section: Introductionmentioning

confidence: 99%

“…Laminin (LN) has previously been shown to be one of the most abundant ECM components within the basement membrane (Wordinger et al 1983, Leardkamolkarn & Abrahamson 1992, Zhao & Luck 1995, Lee et al 1996, Huet et al 1997, van Wezel et al 1998, Alexopoulos et al 2000. In vitro, purified LN used as a culture substratum improves GC survival in rat (Aharoni et al 1996) and sheep (Huet et al 2001) and stimulates their proliferation in sheep (Huet et al 2001). However, the effects of LN on GC steroidogenesis are still the object of controversy since LN enhances P4 secretion in rat (Aten et al 1995, Aharoni et al 1996 and pig (Sites et al 1996), but inhibits it in human .…”

Section: Introductionmentioning

confidence: 99%

“…Thereafter, in preovulatory follicles, GC luteinize into progesterone (P4)-secreting cells in response to the preovulatory surge of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH). It has previously been hypothesized that extracellular matrix (ECM) components present in ovarian follicles might modulate the actions of gonadotropins on these transitions (Gospodarowicz et al 1980, Savion et al 1981, Amsterdam et al 1989, Aten et al 1995, Aharoni et al 1996, Huet et al 1997. Within the ovarian follicles, the outer layer of GC rests on a basement membrane separating the intrafollicular compartment from the theca (Luck 1994).…”

Section: Introductionmentioning

confidence: 99%

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Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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This study aimed to determine the physiological role of laminin (LN) and its receptor, 6 1 integrin, in controlling the functions of granulosa cells (GC) during follicular development in sheep ovary. Immunohistochemistry experiments showed the presence of increasing levels of LN (P<0·0001), and high levels of mature 6 1 integrin in GC layers of healthy antral follicles during the follicular and the preovulatory phases of the estrous cycle. In vitro, the addition of a function-blocking antibody raised against 6 subunit (anti-6 IgG) to the medium of ovine GC cultured on LN impaired cell spreading (P<0·0001), decreased the proliferation rate (P<0·05) and increased the apoptosis rate (P<0·05). Furthermore, addition of anti-6 IgG enhanced estradiol (E2) secretion by GC in the presence or absence of follicle-stimulating hormone (FSH), luteinizing hormone or insulin-like growth factor-I in culture medium (P<0·0001), and inhibited progesterone (P4) secretion in basal conditions or in the presence of low (0·5 ng/ml) FSH concentrations only (P<0·0001). The anti-6 IgG effect was specific to an interaction of LN with 6 1 integrin since it was ineffective on GC cultured on heat-denatured LN, RGD (arginine-glycine-aspartic acid) peptides and non-coated substratum. Hence, this study established that 6 1 integrin 1) was expressed in GC of antral follicles, 2) mediated the actions of LN on survival, proliferation and steroidogenesis of GC, and 3) was able to dramatically modulate P4 and E2 secretion by GC in vitro. It is suggested that during the follicular and the preovulatory phases of the estrous cycle, the increasing levels of LN in GC of large antral follicles might support their final development to ovulation.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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“…The role of integrins has been reported as crucial for maintenance of beta-cell viability and differentiation [13,18,30,31,32]. Studies from our own group have further demonstrated the specific role of integrins α3β1 and α6β1 on rat beta cells [12,33].…”

Section: Discussionmentioning

confidence: 70%

Impact of integrin-matrix matching and inhibition of apoptosis on the survival of purified human beta-cells in vitro

et al. 2002

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Aims/hypothesis. Human islet cells survive poorly in culture and are overgrown by non-endocrine cells. The aims of this study were to sort human beta cells and to develop approaches for their improved survival in culture. Methods. Human islets were infected with recombinant adenovirus expressing green fluorescent protein (GFP) under the control of the rat insulin promoter such that only beta cells expressed GFP. GFP-positive beta cells were sorted by flow cytometry, and expression of select integrins evaluated by RT-PCR. Beta cells were cultured on different extracellular matrices for up to 15 days. Apoptosis was measured by annexin V binding and ELISA. Insulin secretion was measured by ELISA. Results. Sorted beta cells survived less well in culture than unsorted islet cells. This did not appear to be due to adenoviral infection and/or GFP expression. Purified beta cells expressed the integrins α3, α5, α6, αV, β1, but not β4. Of the various matrices tested, sorted beta cells attached and spread best on a lawn of lysed human bladder carcinoma cells (5637 cells). However, survival remained poor. Cell death was decreased but not prevented by continued presence of 10 mmol/l nicotinamide and apoptosis decreased by 24 h incubation with 20 µmol/l Z-VAD. Insulin secretion was maintained over 6 days following treatment with both agents. Conclusions/interpretation. Purification of human beta cells induces marked apoptosis limiting their function and survival in vitro. This was improved by matching the extracellular matrix to the specific expression of integrins and by addition of nicotinamide and Z-VAD. [Diabetologia (2002) 45:841-850]

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Expression and function of the urokinase type plasminogen activator during mouse hemochorial placental development

Teesalu

Blasi

Talarico

1998

Developmental Dynamics

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Differential effect of components of the extracellular matrix on differentiation and apoptosis

Cited by 97 publications

References 53 publications

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Impact of integrin-matrix matching and inhibition of apoptosis on the survival of purified human beta-cells in vitro

Expression and function of the urokinase type plasminogen activator during mouse hemochorial placental development

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