Differential effect of platelet-rich plasma and fetal calf serum on bone marrow-derived human mesenchymal stromal cells expanded in vitro

Abstract

Mesenchymal stromal cells (MSCs) derived from various sources have great potential for use in cell-based therapies. Since the proportion of primary MSCs contained in bone marrow or adipose tissue is low, plastic adherence and in vitro expansion are necessary to expand MSCs prior to clinical application. Human platelet-rich plasma has been introduced as an alternative serum source but functional differences have so far not been described. Here we cultured MSCs derived from human bone marrow in medium supplemented with either 10% fetal calf serum (FCS) or 5% and 10% platelet-rich plasma (PRP) until the first or second passage. Parameters under investigation were cell yield, clonogenicity, phenotype as well as migratory and differentiation potential. In addition, the secretion of SDF-1α and the induced migration of CD34(+) haematopoietic stem cells (HSCs) were investigated with regard to the different serum source. The use of PRP resulted in a significantly higher expansion rate and yield at passages 0 and 1. In addition, the level of secreted SDF-1α was significantly increased in the supernatant of MSCs cultured with FCS instead of human PRP. Consistent with this, the migration capacity of MSCs cultured with 10% FCS as well as their capability to induce the migration of CD34(+) haematopoietic progenitors in a transwell assay was higher. Our results demonstrate that human PRP can be seen as an alternative serum source to FCS for MSC cultivation. However, the requirements of the specific clinical application must be carefully considered before the respective serum source is selected.

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

“…PRP (5% or 10%)-supplemented media heat-inactivated (platelet concentration 79.6 x 10 4 /µL) provided similar results for adipogenic and osteogenic differentiation when compared to 10% FBS (92). Besides, PRP supplemented αMEM also provides osteogenenic, chondrogenic and adipogenic differentiation promoting an increase of cell culture (96)(97)(98)(99)(100)(101)(102).…”

“…An ASC genome gene expression analysis depicted 102 genes were commonly expressed in differentiated ASC being 90 genes, including those responsible for MSC adhesion, high expressed in 10% FBS-supplemented ASC (24), which may be connected to high sensitivity presented to trypsin by MSC expanded in VBD. Changes in surface markers expression has been contradictorily reported in VBD-expanded MSC (25,49,50,92). HS and aPRP provided maintenance of MSC immunophenotype (25).…”

The Stem Cell Potential and Multipotency of Human Adipose Tissue-Derived Stem Cells Vary by Cell Donor and Are Different from Those of Other Types of Stem Cells

“…In a previous study, CD73 and CD105 were present and CD34 was absent [Baptista et al, 2009]. Therefore, all AT-MSCs expressed CD13, CD73, CD90, and CD105 but did not express hematopoietic markers [Jurgens et al, 2008;Bieback et al, 2009;Griesche et al, 2010;Goedecke et al, 2011]. Adipose tissue SVF contains hematopoietic cells [Varma et al, 2007;Faustini et al, 2010], but these cells are eliminated upon subculture (typically, by passage 3).…”

Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience

“…MSC migration is investigated in few studies 11,29,32,38 all but one showed enhanced MSC migration in PRP comparing to FBS 29,32 . Moreover, Murphy 32 et al have compared ucPRP (umbilical cord PRP), aPRP (adult PRP), aPPP and FBS effect in MSC migration and found that all forms of human plasma were much more effective in promoting cell migration than FBS.…”