Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience

Rubio‐Azpeitia, Eva; Andı́a, Isabel

doi:10.11138/mltj/2014.4.1.052

Cited by 57 publications

(54 citation statements)

References 71 publications

(38 reference statements)

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“…This therapy was possible because other researches have demonstrated the benefit of this kind of treatment in similar wounds [12,34,59]. At the same way, after several months of treatment, they could see completely incorporation of the 3D, this without side effects were seen.…”

Section: Introductionmentioning

confidence: 72%

Transcendence of Medicine Regenerative Strategies in the Treatment for Diabetic Foot Ulcers

Díaz-García¹,

Benítez-Arvizu²

2016

J Cancer Diagn

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The Diabetes Mellitus type 2 and one of its complications, the diabetic foot ulcer (DFU), are a great problem of public health that affects millions of people around the world. Due to its complexity, DFUs treatment and handling are expensive and complex. In addition, amputations of lower limbs and death are relatively frequent because conventional treatment cannot avoid them. In other hand, the use of growth factors, 3D scaffolds and mesenchymal stem cells, called regenerative medicine (RM), have created new perspectives about the treatment of this kind of injuries. The RM has demonstrated favorable clinical outcomes not only in the case of DFUs, so that, this new medicine area promises to become an important tool that supports or substitutes to conventional treatment in cases of tissue or organic damage caused by the different chronic and traumatic diseases.

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Section: Introductionmentioning

confidence: 72%

Transcendence of Medicine Regenerative Strategies in the Treatment for Diabetic Foot Ulcers

Díaz-García¹,

Benítez-Arvizu²

2016

J Cancer Diagn

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“…Although the limited supply of fresh platelets is a bottleneck when using human growth supplements to yield large‐scale cellular materials for both cell banking and cell‐based therapies, outdated platelets from human blood donors have recently been shown to be an efficient and reliable resource for producing PL (Glovinski et al, ). Human‐derived PL not only surpasses xenogeneic FBS in terms of bio‐safety but also excels in maintaining the potential of MSCs for proliferation, differentiation and immunomodulation (Chen & Liu, ; Rubio‐Azpeitia & Andia, ). In addition to replacing FBS, PL can be used to supplement culture medium and not only accelerate MSC proliferation but also reduce the time required to harvest the number of cells required for clinical use (Griffiths, Baraniak, Copland, Nerem, & McDevitt, ; Riordan et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…In terms of the effect of PL on tri‐lineage differentiation, discrepancies exist in the results and cell types of different studies. However, in most cases, if not all, PL at least does not interfere with the multipotency of MSCs and often results in an improved biological performance of the resultant cellular materials (Rubio‐Azpeitia & Andia, ). Moreover, MSCs expanded in PL‐supplemented culture medium exhibited preserved immune‐privileged capacities, such as the suppression of T cell proliferation and activation (Flemming et al, ); such features enabled these cells to be used for treating graft‐vs‐host disease in clinical trials (Introna et al, ).…”

Section: Discussionmentioning

confidence: 99%

Human platelet lysate supports the formation of robust human periodontal ligament cell sheets

Tian

et al. 2017

J Tissue Eng Regen Med

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The use of stem cell-derived sheets has become increasingly common in a wide variety of biomedical applications. Although substantial evidence has demonstrated that human platelet lysate (PL) can be used for therapeutic cell expansion, either as a substitute for or as a supplement to xenogeneic fetal bovine serum (FBS), its impact on cell sheet production remains largely unexplored. In this study, we manufactured periodontal ligament stem cell (PDLSC) sheets in vitro by incubating PDLSCs in sheet-induction media supplemented with various ratios of PL and FBS, i.e. 10% PL without FBS, 7.5% PL + 2.5% FBS, 5% PL + 5% FBS, 2.5% PL + 7.5% FBS or 10% FBS without PL. Cultures with the addition of all the designed supplements led to successful cell sheet production. In addition, all the resultant cellular materials exhibited similar expression profiles of matrix-related genes and proteins, such as collagen I, fibronectin and integrin β1. Interestingly, the cell components within sheets generated by media containing both PL and FBS exhibited improved osteogenic potential. Following in vivo transplantation, all sheets supported significant new bone formation. Our data suggest that robust PDLSC sheets can be produced by applying PL as either an alternative or an adjuvant to FBS. Further examination of the relevant influences of human PL that benefit cell behaviour and matrix production will pave the way towards optimized and standardized conditions for cell sheet production.

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“…Use of PRP was then anticipated to improve clinical outcome since it has been reported that PRP enhances MSC proliferation and chondrogenic differentiation [21][22][23] and could further improve cartilage regeneration.…”

Section: Discussionmentioning

confidence: 99%

Combined use of platelet rich plasma and micro-fat in sport and race horses with degenerative joint disease: preliminary clinical study in eight horses

Bembo¹,

Eraud

Philandrianos

et al. 2016

MLTJ

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SummaryBackground: To assess the safety and potential efficacy of a standardized technique consisting of intra-articular injection of 10 cc of a homogeneous mixed product using autologous micro-fat and platelet rich plasma (PRP) (ratio 1:1) in the carpus or the fetlock joint of sport horses presenting degenerative joint disease (DJD). Methods: Eight sport horses with DJD confirmed by radiography and ultrasonography and causing lameness and the impossibility to compete were treated. PRP was prepared after a double centrifugation whereas micro-fat was harvested and purified using a closed system. The two products were connected and mixed by gentle back and forth shaking of the syringes to finally obtain 10 ml of an homogeneous mixed product. Follow up was performed from 5 to 10 months with assessment of AAEP lameness score and return to training and competition.Results: Nine joints were treated with significant improvement of the AAEP lameness score three months after the procedure (p = 0.021). Four horses returned to official competition between 5 to 10 months after the procedure (7.0±2.5) and three of them resumed intensive training between 5 to 9 months (6.3±2.3). No adverse event occurred. Conclusion: This study is a first step in the development of innovative therapy for DJD which combines the potential chondrogenic differentiation of MSCs inside equine adipose tissue with the proliferative effect of growth factors present in PRP.

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Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience

Cited by 57 publications

References 71 publications

Transcendence of Medicine Regenerative Strategies in the Treatment for Diabetic Foot Ulcers

Transcendence of Medicine Regenerative Strategies in the Treatment for Diabetic Foot Ulcers

Human platelet lysate supports the formation of robust human periodontal ligament cell sheets

Combined use of platelet rich plasma and micro-fat in sport and race horses with degenerative joint disease: preliminary clinical study in eight horses

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