Differential effects of acute and chronic zinc (Zn) exposure on hepatic lipid deposition and metabolism in yellow catfish Pelteobagrus fulvidraco

Effects of recombinant human leptin administration on hepatic lipid metabolism in yellow catfish Pelteobagrus fulvidraco: In vivo and in vitro studies

“…Our study showed that PPARa transcription was negatively correlated with lipogenesis (6PGD, G6PD, ME, ICDH and FAS), and positively correlated with CPT-1 activity, supporting the notion. Similarly, other studies reported that the PPARa mRNA abundance was positively correlated to CPT-1 mRNA abundance (Hsu and Hung, 2007;Zheng et al, 2013;Huang et al, 2014). Further studies indicated that PPARa stimulated through a PPRE in the first and second intron of the human and rat CPT-1A gene, respectively (Napal et al, 2005).…”

“…All samples were performed in triplicate and each reaction was verified to contain a single product of the correct size by agarose gel electrophoresis. The relative mRNA expression of genes were calculated with the ''delta-delta Ct'' method (Pfaffl, 2001), where b-actin was amplified to confirm the steady-state level of the mRNA expression of the housekeeping gene and used as reference gene (Gong et al, 2013;Zheng et al, 2013).…”

“…Recently, in our laboratory, we have cloned and obtained cDNA sequences of many genes involved in lipid metabolism and its regulation for yellow catfish, consisting of the leptin and LepR cDNA sequences Gong et al, 2013;Zheng et al, 2013). The present study was conducted to investigate the effects and mechanism of the acute administration of recombinant human leptin (rt-hLEP) on hepatic lipid metabolism for the fish species.…”

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

“…For example, a few lipid inclusions and some deformed mitochondria with clear cristae were observed after Zn stress. Zheng et al (2013) suggested hepatic lipid accumulation is as a result of mitochondria oxidative function injury. Mitochondria is a major site of the generation of cellular ROS (Martínez-Reyes and Cuezva, 2014), its damage would result in the polarization of mitochondrial membrane and increases the production of ROS (Korshunov et al, 1997).…”

PPARα, PPARγ and SREBP-1 pathways mediated waterborne iron (Fe)-induced reduction in hepatic lipid deposition of javelin goby Synechogobius hasta

“…SREBP-1 and PPARγ are key transcriptional factors involved in lipogenesis whereas PPARα plays key roles in the catabolism of fatty acids (Spiegelman and Flier, 2001). Studies suggested that effects of metal elements (such as Cu and Zn) on enzymatic activities and expression of genes mentioned above were concentration-dependent in fish (Zheng et al, 2013Chen et al, 2013aChen et al, , 2013bChen et al, , 2015Huang et al, 2014Huang et al, , 2016Wu et al, 2016a). In addition, investigation into toxicity of metal elements has focused on high-doses exposure, which is a very different situation from the low-doses exposure encountered in the environment by fish.…”