Differential effects of cold acclimation and abscisic acid on free amino acid composition in wheat

Kovács, Zita; Simon‐Sarkadi, Lívia; Sovány, Csongor; Kirsch, Klára; Galiba, Gábor; Kocsy, Gábor

doi:10.1016/j.plantsci.2010.08.010

Cited by 61 publications

(39 citation statements)

References 51 publications

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“…In fact, the major cryoprotectants used in cryopreservation are all compounds we found to be able to form NADES (Mustafa et al, 2011). It has been reported that in wheat (Triticum aestivum), freezing tolerance correlates with a high Pro level (Kovács et al, 2011). This confirms our results in which Pro was seen to be a good candidate to form liquids with organic acids or sugars.…”

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confidence: 90%

Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?

et al. 2011

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supporting

confidence: 90%

Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?

et al. 2011

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“…The expression of transcript of all the genes was analysed with two biological and three technical repeats by RT PCR (Bio Rad CFX96 TM Real Time Detection System) using gene specific primers. The primer sequences for Tacyclophilin, TaSOS1 and TaNHX1 were used as described by Cuin et al (2011) and for P5CR and GOGAT as per Kovács et al (2011). All the primers were synthesized by Sigma Life Sciences.…”

Section: Rna Extraction and Rt-pcr Assaymentioning

confidence: 99%

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Rana

Ram

Nehra

et al. 2016

Cereal Research Communications

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In the present investigation, expression of genes related to Na + exclusion such as salt overly sensitive (TaSOS1) and Na + /H + antiporter (TaNHX1) and proline accumulation such as pyrroline-5-carboxylate reductase (P5CR) and glutamate synthase (GOGAT) was studied in seedlings of Kharchia 65 (Kh 65, salt tolerant) and HD 2009 (sensitive) under salt stress (ECe, 12 dSm -1 ) and controlled conditions. As compared to HD 2009, Kh 65 showed significantly lower accumulation of Na + (p < 0.01) and higher accumulation of proline (p < 0.05) in leaf blade under salt stress. The relative expression of TaSOS1 increased significantly (p < 0.001) in roots of Kh 65 (4.31-fold) while it decreased in HD 2009. There was significantly higher (p < 0.01) relative expression of TaNHX1 (27.57-fold) in leaf and 3.07-fold in roots of Kh 65 as compared to 3.65-and 0.87-fold increase in leaf and roots of HD 2009, respectively, under salt stress. There was significantly (p < 0.05) higher accumulation of proline as compared to HD 2009 in leaf tissues. There was significantly higher (p < 0.01) expression of P5CR (5.23-fold in leaf and 8.77-fold in the root) and glutamate synthase (6.0-fold in roots) in Kh 65 as compared to HD 2009. The study demonstrated that upregulation of genes for Na + exclusion in root and compartmentation in leaf and increased proline concentration are associated with tolerance to salinity stress in wheat. The information will be useful for improving wheat genotypes for salt tolerance.

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“…The accumulation of proline, along with that of other amino acids, osmolytes and hormones, plays a key role in the survival mechanisms of plants subjected to low-temperature conditions (Yelenosky, 1979;Dörffling et al, 1990;Parvanova et al, 2004;Macháčková et al, 2006;Kovács et al, 2011;Kocsy et al, 2011a). Proline augmentation could avert water losses from cells due to extracellular ice formation during cold treatment (Kovács et al, 2011). The results obtained in the present experiments suggested that pretreatment with polyamines could protect wheat seedlings subjected to freezing conditions by an additional increase in the free proline pool.…”

Section: Discussionmentioning

confidence: 55%

“…The concordant accumulation of polyamines and proline in wheat during cold acclimation, reported by Kocsy et al (2011b), is a clue to the probable mechanism by which the application of polyamines could render protection against low temperatures. The accumulation of proline, along with that of other amino acids, osmolytes and hormones, plays a key role in the survival mechanisms of plants subjected to low-temperature conditions (Yelenosky, 1979;Dörffling et al, 1990;Parvanova et al, 2004;Macháčková et al, 2006;Kovács et al, 2011;Kocsy et al, 2011a). Proline augmentation could avert water losses from cells due to extracellular ice formation during cold treatment (Kovács et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Application of natural and synthetic polyamines as growth regulators to improve the freezing tolerance of winter wheat (Triticum aestivum L.)

Todorova

Sergiev

Alexieva

2012

Acta Agronomica Hungarica

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Wheat cultivars were grown as soil culture under normal growth conditions. Twoweek-old seedlings were exposed to 4°C for 6 h and then transferred to -12°C for 24 h in the dark. Twenty-four hours before freezing stress, some of the plants were sprayed with aqueous solutions of spermine, spermidine, putrescine, 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA). The data showed that freezing stress caused a decrease in the fresh weight, chlorophyll content and plant survival rate, accompanied by a simultaneous accumulation of free proline and the enhanced leakage of electrolytes. Preliminary treatment with polyamines caused a decline in electrolyte leakage and a considerable augmentation in proline quantity, indicating that the compounds are capable of preventing frost injury. Additionally, the foliar application of polyamines retarded the destruction of chlorophyll, and lessened fresh weight losses due to freezing stress. The synthetic triamine DETA was the most effective, having the most pronounced action in all the experiments, followed by the tetraamine spermine. The application of polyamines to wheat crops could be a promising approach for improving plant growth under unfavourable growth conditions, including freezing temperatures. The results demonstrate that treatment with polyamines could protect winter wheat by reducing the stress injuries caused by subzero temperatures.

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Differential effects of cold acclimation and abscisic acid on free amino acid composition in wheat

Cited by 61 publications

References 51 publications

Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?

Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Application of natural and synthetic polyamines as growth regulators to improve the freezing tolerance of winter wheat (Triticum aestivum L.)

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Differential effects of cold acclimation and abscisic acid on free amino acid composition in wheat

Cited by 61 publications

References 51 publications

Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?

Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?

Expression of genes related to Na+exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Application of natural and synthetic polyamines as growth regulators to improve the freezing tolerance of winter wheat (Triticum aestivum L.)

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Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress