Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells

Kemppainen, Jessica M.; Hollister, Scott J.

doi:10.1016/j.biomaterials.2009.09.041

Cited by 114 publications

(94 citation statements)

References 37 publications

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“…20 The low and high permeability designs have porosities of 53.46% and 70%, and surface areas of 317.69 and 260.52 mm 2 , respectively. The designs and associated properties are displayed in Table 1.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Mitsak

Kemppainen

Harris

et al. 2011

Tissue Engineering Part A

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157

114

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Successful bone tissue engineering depends on the scaffold's ability to allow nutrient diffusion to and waste removal from the regeneration site, as well as provide an appropriate mechanical environment. Since bone is highly vascularized, scaffolds that provide greater mass transport may support increased bone regeneration. Permeability encompasses the salient features of three-dimensional porous scaffold architecture effects on scaffold mass transport. We hypothesized that higher permeability scaffolds will enhance bone regeneration for a given cell seeding density. We manufactured poly-e-caprolactone scaffolds, designed to have the same internal pore design and either a low permeability (0.688 · 10 /N-s), respectively. Scaffolds were seeded with bone morphogenic protein-7-transduced human gingival fibroblasts and implanted subcutaneously in immune-compromised mice for 4 and 8 weeks. Micro-CT evaluation showed better bone penetration into high permeability scaffolds, with blood vessel infiltration visible at 4 weeks. Compression testing showed that scaffold design had more influence on elastic modulus than time point did and that bone tissue infiltration increased the mechanical properties of the high permeability scaffolds at 8 weeks. These results suggest that for polycaprolactone, a more permeable scaffold with regular architecture is best for in vivo bone regeneration. This finding is an important step toward the end goal of optimizing a scaffold for bone tissue engineering.

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Section: Scaffold Fabricationmentioning

confidence: 99%

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Mitsak

Kemppainen

Harris

et al. 2011

Tissue Engineering Part A

Self Cite

157

114

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“…Other authors observed the limited role of the macro pores in a PCL scaffold for bone regeneration and suggested further studies of the scaffold properties like interconnectivity and permeability 23 . In other works it is seen that a low permeability in a porous PCL scaffold enhanced chondrogenesis in vitro with primary chondrocytes 24 and by measuring the permeability of the scaffold with cells it is seen how the permeability diminishes from seeding as tissue grows 25 . Other authors have used 3D…”

Section: Introductionmentioning

confidence: 96%

Relationship between micro-porosity, water permeability and mechanical behavior in scaffolds for cartilage engineering

Vikingsson

Claessens

Gómez-Tejedor

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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ElsevierVikingsson, LKA.; Claessens, B.; Gómez Tejedor, JA.; Gallego Ferrer, G.; Gómez Ribelles, JL. (2015). Relationship between micro-porosity, water permeability and mechanical behavior in scaffolds for cartilage engineering. that the stress response of the scaffold/hydrogel construct is a synergic effect from the performance of each of the components. This is interesting since it predicts that the in vivo outcome of the scaffold is not only depending on the material architecture but also the growing tissue inside the scaffolds pores. On the other hand, the confined compression results show that the compliance of the scaffold is mainly controlled by the micro porosity of the scaffold and less by the hydrogel density in the scaffold pores. These conclusions bring together valuable information for customizing the optimal scaffold and to predict the in vivo mechanical behavior.

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“…Indeed, strictly controlled structural and mass transport properties are required for load bearing after transplantation, maintaining cell retention, viability, and differentiation. 4,25,26 Accordingly, two types of scaffolds having different mechanical and morphological characteristics were employed 14 as models for evaluating the influential parameters for cell seeding. A CRIS technique was used for cell seeding inside these preformed scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…32,33 On the other hand, it has been shown that cartilaginous matrix synthesis by chondrocytes is enhanced inside scaffolds having smaller pore size and lower permeability (mimicking more similarly in vivo cartilage environment). 26,34 Taken together, a trade-off between seeding outcome and chondrogenesis should be considered for cartilage TE when designing scaffold permeability, whereas this is not the case for osteogenesis in bone TE.…”

Section: Effective Cell Seeding Technique 493mentioning

confidence: 99%

Development of an Effective Cell Seeding Technique: Simulation, Implementation, and Analysis of Contributing Factors

Nasrollahzadeh

Applegate

Pioletti

2017

Tissue Engineering Part C: Methods

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Cell seeding in a biomaterial is an important process for tissue engineering applications. It helps to modulate tissue formation or to control initial conditions for mechanobiological studies. The compression release-induced suction (CRIS) seeding technique leads to active infiltration of the cell suspension toward the central region of the scaffold. Its effectiveness, however, may significantly vary depending on several controlling factors such as the rate of loading and unloading or scaffold architecture. We utilized a 2D axisymmetric finite element model to numerically evaluate the influence of a seeding loading regime on suction pressure and infiltration velocity of the cell suspension. The in vitro application of optimized CRIS seeding obtained from simulation showed significant effectiveness over a static seeding method. As simulation results predicted, the permeability of the scaffold directly influenced CRIS seeding effectiveness in vitro. By supplementing an optimized CRIS loading regime with slow rotation after seeding treatment, cell distribution through thickness was improved especially for scaffolds showing low permeability. Finally, we systematically analyzed the relative importance of permeability, thickness, or coating on cell seeding efficiency and uniformity using a full factorial design of experiments. We observed that permeability has a higher impact on the CRIS seeding than scaffold coating and thickness.

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Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells

Cited by 114 publications

References 37 publications

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Relationship between micro-porosity, water permeability and mechanical behavior in scaffolds for cartilage engineering

Development of an Effective Cell Seeding Technique: Simulation, Implementation, and Analysis of Contributing Factors

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