Differential effects of Zincum metallicum on cell models

Gonçalves, Jenifer Pendiuk; Santos, Maria Luiza Ferreira dos; Rossi, Gustavo Rodrigues; Gagosian, Viviana Stephanie Costa; Oliveira, Carolina Camargo de

doi:10.1016/j.homp.2017.02.004

Cited by 7 publications

(10 citation statements)

References 59 publications

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“…demonstrated that the homeopathic mother tincture of Phytolacca decandra induces apoptosis of A375 melanoma cells by activating caspases-mediated signaling and ROS generation 24 . On the other hand, no effect was observed on melanoma cells with Zincum metallicum 25 . Although these previous studies are encouraging, the particular action of the mechanism remains unclear.…”

Section: Discussionmentioning

confidence: 87%

Low-diluted Phenacetinum disrupted the melanoma cancer cell migration

Fuselier

Terryn

Berquand

et al. 2019

Sci Rep

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Dynamic and reciprocal interactions generated by the communication between tumor cells and their matrix microenvironment, play a major role in the progression of a tumor. Indeed, the adhesion of specific sites to matrix components, associated with the repeated and coordinated formation of membrane protrusions, allow tumor cells to move along a determined pathway. Our study analyzed the mechanism of action of low-diluted Phenacetinum on murine cutaneous melanoma process in a fibronectin matrix environment. We demonstrated a reduction of dispersed cell migration, early and for as long as 24 h, by altering the formation of cell protrusions. Moreover, low-diluted Phenacetinum decreased cell stiffness highly on peripheral areas, due to a disruption of actin filaments located just under the plasma membrane. Finally, it modified the structure of the plasma membrane by accumulating large ordered lipid domains and disrupted B16 cell migration by a likely shift in the balance between ordered and disordered lipid phases. Whereas the correlation between the excess of lipid raft and cytoskeleton disrupting is not as yet established, it is clear that low-diluted Phenacetinum acts on the actin cytoskeleton organization, as confirmed by a decrease of cell stiffness affecting ultimately the establishment of an effective migration process.

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Section: Discussionmentioning

confidence: 87%

Low-diluted Phenacetinum disrupted the melanoma cancer cell migration

Fuselier

Terryn

Berquand

et al. 2019

Sci Rep

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“…[45][46][47] Macrophage cultures are very sensitive for detecting the effect of high dilutions but are also sensitive for detecting such solvent changes as well, concerning elements related to cell oxidative metabolism. [48][49][50] Such biochemical interference could lead to non-specific results-that is, not related to the Similia principle itself, but to the physico-chemical changes of the solvent due to agitation. Similar solventdependent effects can also be easily seen in vegetal models.…”

Section: Discussionmentioning

confidence: 99%

“…The findings (1) and (2) above are parasite and/or macrophage features, whose mechanisms need to be elucidated in further studies, though they are probably related to the specific expression of certain genes, as already seen in other similar studies using macrophages invitro. 17,18,[48][49][50] From a practical point of view, putative overlapping between the pro-inflammatory effects of succussed water, including the induction of inflammatory cytokines and chemokines (such as MCP-1, MIP-1β and TNFα) and the specific effects of Phos 200cH (acute expression of RANTES and lysosome activation), would also be present in clinical situations. Thus, an important consideration emerges: perhaps homeopathy acts through different and simultaneous mechanisms, with diverse degrees of specificity, which could be in accordance with the systemic effects observed in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Effects of Homeopathic Phosphorus on Encephalitozoon cuniculi-Infected Macrophages In-Vitro

et al. 2019

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Introduction Encephalitozoon cuniculi (E. cuniculi), a fungus that acts as an intracellular pathogen, causes a marked neurological syndrome in many host species and is a zoonotic concern. Although no well-established treatment for this syndrome is known, previous successful clinical experience using homeopathic phosphorus has been described in which symptom remission with no mortality occurred in 40/42 animals by means of unknown immunological mechanisms. The latter observation was the main motivation for this study. Objective To verify, in an in-vitro model, if macrophages infected with E. cuniculi can change in function after treatment with different potencies of phosphorus. Materials and Methods RAW 264.7 macrophages were infected with E. cuniculi in-vitro and treated with various homeopathic potencies of phosphorus. The vehicle was used as a control solution (0.06% succussed ethanol). After 1 and 24 hours, the following parameters were analyzed: parasite internalization (by the Calcofluor staining method), lysosome activity (by the acridine orange method), cytokine/chemokine production (by the MAGPIX system), and cell ultrastructure. Automatic image analysis was used when applicable, and the experiments were performed in triplicate. Results Treatment with vehicle alone increased interleukin (IL)-6, tumor necrosis factor alpha and monocyte chemotactic protein -1 production (p ≤ 0.05) and reduced the number of internalized parasites (p ≤ 0.001). A progressive and time-dependent increase in RANTES (regulated on activation, normal T-cell expressed and secreted) and lysosome activity (p ≤ 0.002) was observed only after treatment with the highest potency of phosphorus (Phos 200cH), together with decreased apoptosis rate, intense parasite digestion, and the presence of non-internalized spores. Conclusions Phos 200 cH has a modulatory action on the activity of infected macrophages, especially a specific increase in RANTES, a key element in the prognosis of E. cuniculi-infected and of immunosuppressed patients bearing infections.

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“…Finding cost-effective treatments for selected diseases has been a priority in our research group. Over the past years, we have investigated, in vitro (24)(25)(26)(27)(28)(29)(30)(31) and in vivo (32)(33)(34)(35)(36), the effects of highly diluted natural complexes (HDNCs). Lately, we have focused on two specific HDNCs, namely M1 and M8.…”

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

In vitro attenuation of classic metastatic melanoma‑related features by highly diluted natural complexes: Molecular and functional analyses

et al. 2019

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Metastasis is responsible for the majority of deaths among patients with malignant melanoma. Despite recent advances, the majority of current and modern therapies are ineffective and/or financially unfeasible. Thus, in this study, we investigated two low-cost highly-diluted natural complexes (HDNCs) that have been shown to be effective against malignant melanoma in a murine model in vivo. The aim of this study was to determine the mechanisms through which these HDNCs directly affect melanoma cells, either alone or in an artificial tumor microenvironment, suppressing the metastatic phenotype, thus explaining previous in vivo effects. For this purpose, HDNC in vitro treatments of B16-F10 melanoma cells, alone or in co-culture with Balb/3T3 fibroblasts, were carried out. Molecular biology techniques and standard functional assays were used to assess the changes in molecule expression and in cell behaviors related to the metastatic phenotype. Melanoma progression features were found to be regulated by HDNCs. Molecules related to cell adhesion (N-cadherin, β1-integrin and CD44), and migration, extracellular matrix remodeling and angiogenesis were modulated. The cell migratory, invasive and clonogenic capacities were reduced by the HDNCs. No loss of cell proliferation or viability were observed. On the whole, the findings of this study indicate that HDNCs directly reprogram, molecularly and functionally, melanoma cells in vitro, modulating their metastatic phenotype. Such findings are likely to be responsible for the attenuation of tumor growth and lung colonization previously observed in vivo.

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Differential effects of Zincum metallicum on cell models

Cited by 7 publications

References 59 publications

Low-diluted Phenacetinum disrupted the melanoma cancer cell migration

Low-diluted Phenacetinum disrupted the melanoma cancer cell migration

Effects of Homeopathic Phosphorus on Encephalitozoon cuniculi-Infected Macrophages In-Vitro

In vitro attenuation of classic metastatic melanoma‑related features by highly diluted natural complexes: Molecular and functional analyses

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