Differential expression analyses of host genes involved in systemic infection of Tomato leaf curl New Delhi virus (ToLCNDV)

Naqvi, Afsar Raza; Sarwat, Maryam; Pradhan, Bhubaneswar; Choudhury, Nirupam Roy; Haq, Qazi Mohd. Rizwanul; Mukherjee, Sunil Kumar

doi:10.1016/j.virusres.2011.05.002

Cited by 23 publications

(12 citation statements)

References 20 publications

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“…Both geminiviruses activate a generic response to biotic stress, involving PR proteins, heat shock proteins and glutatione-S transferases. This host reaction was also highlighted using the mRNA-seq approach in pepper plants infected by the PepGMV [18] and, by subtractive hybridization, in tomato plants infected by ToLCNDV [17]. Similar plant response to biotic stresses was observed in the majority of RNA virus infections [13], [74].…”

Section: Discussionmentioning

confidence: 68%

“…Both TYLCSV and CabLCV affect ET metabolism, mainly activating the 1-aminocyclopropane-1-carboxylate oxidase (ACCO), which regulates the final step of ET biosynthesis [61]. This enzyme is also induced by ToLCNDV [17] and PepGMV [18]. On the other hand, TYLCSV down-regulated (FC = 0.18) the transcript coding for the 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), which catalyses the rate-limiting step in the ethylene biosynthetic pathway [61], while no differential expression of this gene was observed in Arabidopsis infected by CabLCV or by SACMV.…”

Section: Discussionmentioning

confidence: 99%

“…Up to now, several studies have been performed to characterize the transcriptional responses of host plants to RNA viruses [12], [13], [14], [15], [16], but only a few dealt with the global impact of geminiviruses on host transcriptome. Naqvi and coworkers [17], by using a subtractive hybridization approach, identified 20 differentially expressed sequence tags (ESTs) induced by the bipartite Tomato yellow leaf curl New Delhi virus (ToLCNDV) in tomato. Góngora-Castillo and co-workers [18] used mRNA-seq technology to compare the transcriptome of recovered and not-recovered pepper leaves infected by the bipartite phloem-limited Pepper golden mosaic virus (PepGMV).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection

et al. 2014

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Tomato yellow leaf curl Sardinia virus (TYLCSV), a DNA virus belonging to the genus Begomovirus, causes severe losses in tomato crops. It infects only a limited number of cells in the vascular tissues, making difficult to detect changes in host gene expression linked to its presence. Here we present the first microarray study of transcriptional changes induced by the phloem-limited geminivirus TYLCSV infecting tomato, its natural host. The analysis was performed on the midrib of mature leaves, a material naturally enriched in vascular tissues. A total of 2206 genes were up-regulated and 1398 were down-regulated in infected plants, with an overrepresentation of genes involved in hormone metabolism and responses, nucleic acid metabolism, regulation of transcription, ubiquitin-proteasome pathway and autophagy among those up-regulated, and in primary and secondary metabolism, phosphorylation, transcription and methylation-dependent chromatin silencing among those down-regulated. Our analysis showed a series of responses, such as the induction of GA- and ABA-responsive genes, the activation of the autophagic process and the fine tuning of the plant immune system, observed only in TYLCSV-tomato compatible interaction so far. On the other hand, comparisons with transcriptional changes observed in other geminivirus-plant interactions highlighted common host responses consisting in the deregulation of biotic stress responsive genes, key enzymes in the ethylene biosynthesis and methylation cycle, components of the ubiquitin proteasome system and DNA polymerases II. The involvement of conserved miRNAs and of solanaceous- and tomato-specific miRNAs in geminivirus infection, investigated by integrating differential gene expression data with miRNA targeting data, is discussed.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection

et al. 2014

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“…Furthermore, plant pathogen interaction or the expression of PR-proteins in general might also be influenced by plant age and surrounding environmental conditions [36]–[40]. Former studies reported differences in PR-protein RNA accumulation after virus attack [3], [41]–[43], but mostly they refer to hours or a few days after the infection. Such early responses have also been observed in young tomato plants infected with PepMV [12]; but one has to consider that these studies focused on leaf material.…”

Section: Discussionmentioning

confidence: 99%

Pepino mosaic virus Infection of Tomato Affects Allergen Expression, but Not the Allergenic Potential of Fruits

Welter

Dölle

Lehmann³

et al. 2013

PLoS ONE

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The plant pathogen Pepino mosaic virus (PepMV) is a major disease of greenhouse tomato crops worldwide. Plant pathogens can induce expression of defence- or pathogenesis-related proteins, including identified allergens. Therefore we hypothesised that PepMV infection results in the expression of allergens leading to a higher allergenic potential of tomato fruits. Transcript level analyses showed differential expression of 17 known and putative tomato fruit allergen encoding genes at early and late time points after PepMV inoculation, but no general induction was detected. Immunoblot analyses were conducted and IgEs from a serum pool of tomato allergic subjects reacted with 20 proteins, of which ten have not yet been described. In parallel, skin prick tests with a group of tomato allergic subjects did not show a general difference between PepMV infected and non-infected tomato fruits and basophil activation tests confirmed these results. In summary, PepMV infection of tomato plants can lead to long-lasting up-regulation of particular allergens in fruits, but the hypothesis that this results in a higher allergenic potential of the fruits proved invalid.

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“…Although the transcriptional modulation of LRR-RLK genes in response to virus infection has been reported for a number of plantviral pathosystems (Naqvi et al 2011;Mandadi and Scholthof 2012;Chen et al 2013;Czosnek et al 2013;Zheng et al 2013;Andolfo et al 2014), their possible role in antiviral defense remains speculative. These genes are induced by virus infection in most cases, but repression has In this study, we focused on SlSOBIR1, a tomato LRR-RLK implicated in plant immunity and described to be a crucial interactor for RLP-mediated resistance against fungal pathogens (Liebrand et al 2013a, b).…”

Section: Discussionmentioning

confidence: 99%

The receptor-like kinase SlSOBIR1 is differentially modulated by virus infection but its overexpression in tobacco has no significant impact on virus accumulation

et al. 2015

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The role of the tomato receptor-like kinase SlSOBIR1 in antiviral defense was investigated. SlSOBIR1 was transcriptionally modulated by unrelated viruses but its ectopic expression had no effect on virus accumulation. Leucine-rich repeat receptor-like kinases (LRR-RLK) constitute a diverse group of proteins allowing the cell to recognize and respond to the extracellular environment. In the present study we focused on a gene encoding a tomato LRR-RLK (named SlSOBIR1) involved in the host defense against fungal pathogens. Curiously, SlSOBIR1 has been previously reported to be down-regulated by Pepper yellow mosaic virus (PepYMV) infection. Here, we show that SlSOBIR1 is responsive to wounding and differentially modulated by unrelated virus infection, i.e., down-regulated by PepYMV and up-regulated by Tomato chlorotic spot virus (TCSV). Despite these divergent expression profiles, SlSOBIR1 overexpression in transgenic tobacco plants had no evident effect on TCSV and PepYMV accumulation. On the other hand, overexpression of SlSOBIR1 significantly increased the expression of selected defense genes (PR-1a and PR-6) and exacerbated superoxide production in wounded leaves. Our data indicate that the observed modulation of SlSOBIR1 expression is probably triggered by secondary effects of the virus infection process and suggest that SlSOBIR1 is not directly involved in antiviral signaling response.

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Differential expression analyses of host genes involved in systemic infection of Tomato leaf curl New Delhi virus (ToLCNDV)

Cited by 23 publications

References 20 publications

Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection

Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection

Pepino mosaic virus Infection of Tomato Affects Allergen Expression, but Not the Allergenic Potential of Fruits

The receptor-like kinase SlSOBIR1 is differentially modulated by virus infection but its overexpression in tobacco has no significant impact on virus accumulation

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