Differential expression and functional constraint of PRL-2 in hibernating bat

Yuan, Lihong; Chen, Jinping; Lin, Benfu; Zhang, Junpeng; Zhang, Shuyi

doi:10.1016/j.cbpb.2007.07.005

Cited by 3 publications

(3 citation statements)

References 47 publications

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“…PRL-2 transcript is upregulated in fibroblasts upon stimulation with conditioned medium from the LnCaP prostate cancer cell line, and conversely, it is downregulated in LnCaP cells exposed to conditioned medium from fibroblasts [22]. Also, PRL-2 expression is modulated in tissues of hibernating vs active bats [83].…”

Section: Prl Expressionmentioning

confidence: 97%

PRL PTPs: mediators and markers of cancer progression

Bessette

Qiu

Pallen

2008

Cancer Metastasis Rev

179

218

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Aberrant protein tyrosine phosphorylation resulting from the altered activity of protein tyrosine phosphatases (PTPs) is increasingly being implicated in the genesis and progression of human cancer. Accumulating evidence indicates that the dysregulated expression of members of the phosphatase of regenerating liver (PRL) subgroup of PTPs is linked to these processes. Enhanced expression of the PRLs, notably PRL-1 and PRL-3, promotes the acquisition of cellular properties that confer tumorigenic and metastatic abilities. Up-regulation of PRL-3 is associated with the progression and eventual metastasis of several types of human cancer. Indeed, PRL-3 shows promise as a biomarker and prognostic indicator in colorectal, breast, and gastric cancers. However, the substrates and molecular mechanisms of action of the PRLs have remained elusive. Recent findings indicate that PRLs may function in regulating cell adhesion structures to effect epithelial-mesenchymal transition. The identification of PRL substrates is key to understanding their roles in cancer progression and exploiting their potential as exciting new therapeutic targets for cancer treatment.

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Section: Prl Expressionmentioning

confidence: 97%

PRL PTPs: mediators and markers of cancer progression

Bessette

Qiu

Pallen

2008

Cancer Metastasis Rev

179

218

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“…In order to validate the accuracy of the sequencing results, we aligned the published gene sequences of M. pilosus with Isoform sequences. Several genes were identified by referring to the relevant papers concerning M. pilosus, including hearing-related genes GJB6 (GenBank: GU326374.1) [36] and SK2 (GenBank: MT822709.1), the immune-related gene DPP4 (GenBank: MH345673.1) [37], and metabolism-related genes Glul (GenBank: KJ561605.1), ND1 (GenBank: AB106586.1) [38], and PRL-2 (GenBank: EF534354.1) [39]. For comparison with the isoform sequences according to the GeneBank numbers in published papers, the whole CDS sequences were downloaded from the NCBI database.…”

Section: Validating the Accuracy Of Sequencing Resultsmentioning

confidence: 99%

Full-Length Transcriptome of Myotis pilosus as a Reference Resource and Mining of Auditory and Immune Related Genes

Wang

Bao

et al. 2022

IJMS

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Rickett’s big-footed bat, Myotis pilosus, which belongs to the family Vespertilionida, is the only known piscivorous bat in East Asia. Accurate whole genome and transcriptome annotations are essential for the study of bat biological evolution. The lack of a whole genome for M. pilosus has limited our understanding of the molecular mechanisms underlying the species’ evolution, echolocation, and immune response. In the present work, we sequenced the entire transcriptome using error-corrected PacBio single-molecule real-time (SMRT) data. Then, a total of 40 GB of subreads were generated, including 29,991 full-length non-chimeric (FLNC) sequences. After correction by Illumina short reads and de-redundancy, we obtained 26,717 error-corrected isoforms with an average length of 3018.91 bp and an N50 length of 3447 bp. A total of 1528 alternative splicing (AS) events were detected by transcriptome structural analysis. Furthermore, 1032 putative transcription factors (TFs) were identified, with additional identification of several long non-coding RNAs (lncRNAs) with high confidence. Moreover, several key genes, including PRL-2, DPP4, Glul, and ND1 were also identified as being associated with metabolism, immunity, nervous system processes, and auditory perception. A multitude of pattern recognition receptors was identified, including NLR, RLR, SRCR, the antiviral molecule IRF3, and the IFN receptor subunit IFNAR1. High-quality reference genomes at the transcriptome level may be used to quantify gene or transcript expression, evaluate alternative splicing levels, identify novel transcripts, and enhance genome annotation in bats.

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“…The three-step thermocycling conditions used were as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 10 s, annealing temperature of DaAqps each pair of primers for 10 s, 72°C for 20 s; melting curve analysis at 95°C for 10 s, 65°C to 95°C in increments of 0.55°C for 5 s. Three biological and three technical replicates were included to ensure reproducibility. Relative expression of DaAqps was determined using the 2 –ΔΔCt method (Livak and Schmittgen, 2001; Yuan et al, 2007; Dai et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Identification, Expression Patterns and RNA Interference of Aquaporins in Dendroctonus armandi (Coleoptera: Scolytinae) Larvae During Overwintering

Dai

Gao

et al. 2019

Front. Physiol.

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The ability to survive annual temperature minima could be a key determinant of distribution limits for insects under global climate change. Recent studies have suggested that insect aquaporins are indispensable for cellular water management under conditions that lead to dehydration and cold stress. Aquaporins are integral membrane water channel proteins in the major intrinsic protein superfamily and promote selected solutes and the movement of water across biological membranes. We cloned and characterized nine full-length aquaporins from Dendroctonus armandi (DaAqps), the most destructive forest pest in the Qinling Mountains of Shaanxi Province, China. Eight of the DaAqps belong to three classical aquaporin grades, including the Drosophila integral protein, the Pyrocoelia rufa integral protein, the entomoglyceroporins and one that belongs to the unorthodox grade of aquaporin 12-like channels. The DaAqps were increasingly expressed during different developmental stages and in different larval tissues, and expression peaked in mid-winter. They were tested under cold conditions for different lengths of time, and the expression of almost all DaAqps was down regulated with decreasing temperatures and long-term exposure to cold conditions. However, when the lowest temperatures were reached, the levels were immediately upregulated. These genes indicate that cold tolerance can improve through mortality responses at low temperatures after RNA interference of DaAqps. In our study, we analyzed the molecular response, expression patterns, and RNA interference of DaAqps and clarified the crucial role of protective compounds (aquaporins) underlying D. armandi cold tolerance and provide a new pest control method.

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Differential expression and functional constraint of PRL-2 in hibernating bat

Cited by 3 publications

References 47 publications

PRL PTPs: mediators and markers of cancer progression

PRL PTPs: mediators and markers of cancer progression

Full-Length Transcriptome of Myotis pilosus as a Reference Resource and Mining of Auditory and Immune Related Genes

Identification, Expression Patterns and RNA Interference of Aquaporins in Dendroctonus armandi (Coleoptera: Scolytinae) Larvae During Overwintering

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