Differential expression and regulation of prohibitin during curcumin-induced apoptosis of immortalized human epidermal HaCaT cells

Yang, Haibo; Song, Wei; Chen, Lan‐Ying; Li, Qifu; Shi, Shengqing; Kong, H. S.; Chen, Pu

doi:10.3892/ijmm.2014.1621

Cited by 16 publications

(8 citation statements)

References 25 publications

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“…These findings are consistent with the observations from an earlier study, in which curcumin was shown to have no effect on fibroblast motility [40]. Other researches have even exhibited that curcumin inhibits cell proliferation and proposed that the inhibition is mediated through the regulation of prohibitin (PHB) in the HaCaT cells [41,42,43].…”

Section: Discussionsupporting

confidence: 91%

Wound Healing Property of Curcuminoids as a Microcapsule-Incorporated Cream

et al. 2019

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Curcuminoids have been used for the management of burns and wound healing in traditional Chinese medicine practices but the wide application of curcuminoids as a healing agent for wounds has always been a known problem due to their poor solubility, bioavailability, colour staining properties, as well as due to their intense photosensitivity and the need for further formulation approaches to maximise their various properties in order for them to considerably contribute towards the wound healing process. In the present study, a complex coacervation microencapsulation was used to encapsulate curcuminoids using gelatin B and chitosan. This study also focused on studying and confirming the potential of curcuminoids in a microencapsulated form as a wound healing agent. The potential of curcuminoids for wound management was evaluated using an in vitro human keratinocyte cell (HaCaT) model and the in vivo heater-inflicted burn wound model, providing evidence that the antioxidant activities of both forms of curcuminoids, encapsulated or not, are higher than those of butylated hydroxyanisole and butylated hydroxytoluene in trolox equivalent antioxidant capacity (TEAC) and (2,2-diphenyl-1-picryl-hydrazyl-hydrate) (DPPH) studies. However, curcuminoids did not have much impact towards cell migration and proliferation in comparison with the negative control in the in vitro HaCaT study. The micoencapsulation formulation was shown to significantly influence wound healing in terms of increasing the wound contraction rate, hydroxyproline synthesis, and greater epithelialisation, which in turn provides strong justification for the incorporation of the microencapsulated formulation of curcuminoids as a topical treatment for burns and wound healing management as it has the potential to act as a crucial wound healing agent in healthcare settings.

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Section: Discussionsupporting

confidence: 91%

Wound Healing Property of Curcuminoids as a Microcapsule-Incorporated Cream

et al. 2019

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“…L6 cells were washed twice with PBS and fixed with 2.5% glutaraldehyde for 30 min. Then it was permeabilized with 1% Triton X‐100 for 10 min, followed by staining with 0.2% Coomassie brilliant blue for 30 min, washed in deionized water, and observed at last (Yang et al, ).…”

Section: Methodsmentioning

confidence: 99%

Coriolus versicolor aqueous extract ameliorates insulin resistance with PI3K/Akt and p38 MAPK signaling pathways involved in diabetic skeletal muscle

Xian

Che

Qin

et al. 2017

Phytotherapy Research

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Patients with type 2 diabetes mellitus (T2DM) are usually with poor immunity and easier to suffer from cancer and microbial infections. Herein, we report an efficient anti-diabetic medicinal mushroom, Coriolus versicolor (CV). This study aimed to investigate the anti-diabetic and anti-insulin-resistance effects of CV aqueous extract in myoblasts (L6 cells) and skeletal muscle of T2DM rat. Our results showed that CV extract treatment significantly reduced blood glucose levels of T2DM rats, whereas CV extract increased glucose consumption in insulin resistant L6 cells. Besides, the translocation and expression of glucose transporter 4 were enhanced by CV extract, which indicated that CV extract was effective in diabetic skeletal muscle. Moreover, CV extract treatments resulted in remarkable anti-insulin-resistance effects, which was reflected by the change of gene and protein expression levels in PI3K/Akt and p38 MAPK pathways. PI3K inhibitor, LY29004, and p38 MAPK inhibitor, SB203580 confirmed it further. In conclusion, our results demonstrated that the CV extract exhibited anti-diabetic and anti-insulin-resistance effects in diabetic skeletal muscle, and the effects were mediated by PI3K/Akt and p38 MAPK pathways. These findings are remarkable when considering the use of commercially available CV by diabetic patients who also suffer from cancer or microbial infections.

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“…PHB1 located in the inner mitochondrial membrane maintains mitochondrial stability by interacting with PHB2 to form a PHB1/PHB2 complex when mitochondria encounter metabolic stress 6 , 28 – 30 . This process modulates the balance between mitochondrial fusion and fission events 31 , 32 , thus maintaining a healthy mitochondrial network that protects cells from mitochondria-related apoptosis 7 , 33 , 34 . Former studies reported that loss of PHB1 and PHB2 in podocytes disrupts the activation of mTORC1 and inhibits kidney filtration 31 , 35 .…”

Section: Location and Function Of Phb1 And Phb2mentioning

confidence: 99%

Significance of prohibitin domain family in tumorigenesis and its implication in cancer diagnosis and treatment

Yang

2018

Cell Death Dis

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Prohibitin (PHB) was originally isolated and characterized as an anti-proliferative gene in rat liver. The evolutionarily conserved PHB gene encodes two human protein isoforms with molecular weights of ~33 kDa, PHB1 and PHB2. PHB1 and PHB2 belong to the prohibitin domain family, and both are widely distributed in different cellular compartments such as the mitochondria, nucleus, and cell membrane. Most studies have confirmed differential expression of PHB1 and PHB2 in cancers compared to corresponding normal tissues. Furthermore, studies verified that PHB1 and PHB2 are involved in the biological processes of tumorigenesis, including cancer cell proliferation, apoptosis, and metastasis. Two small molecule inhibitors, Rocaglamide (RocA) and fluorizoline, derived from medicinal plants, were demonstrated to interact directly with PHB1 and thus inhibit the interaction of PHB with Raf-1, impeding Raf-1/ERK signaling cascades and significantly suppressing cancer cell metastasis. In addition, a short peptide ERAP and a natural product xanthohumol were shown to target PHB2 directly and prohibit cancer progression in estrogen-dependent cancers. As more efficient biomarkers and targets are urgently needed for cancer diagnosis and treatment, here we summarize the functional role of prohibitin domain family proteins, focusing on PHB1 and PHB2 in tumorigenesis and cancer development, with the expectation that targeting the prohibitin domain family will offer more clues for cancer therapy.

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Differential expression and regulation of prohibitin during curcumin-induced apoptosis of immortalized human epidermal HaCaT cells

Cited by 16 publications

References 25 publications

Wound Healing Property of Curcuminoids as a Microcapsule-Incorporated Cream

Wound Healing Property of Curcuminoids as a Microcapsule-Incorporated Cream

Coriolus versicolor aqueous extract ameliorates insulin resistance with PI3K/Akt and p38 MAPK signaling pathways involved in diabetic skeletal muscle

Significance of prohibitin domain family in tumorigenesis and its implication in cancer diagnosis and treatment

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