Differential expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) during flower and fruit development of Bixa orellana

Narváez, José A.; Canché, Blondy B. Canto; Pérez, Patricia Flores; Rivera‐Madrid, Renata

doi:10.1078/0176-1617-00614

Cited by 14 publications

(5 citation statements)

References 38 publications

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“…miltiorrhiza , HMGR expression was detected in roots, stems and leaves but highest expression was found in roots, followed by stem and leaf tissues 28 . In, this study, high expression levels in flower tissues indicate that OkHMGR may have role in growth and development of flower or metabolite accumulation in inflorescence 29 (Fig. 4A ) as HMGR is involved in the synthesis of diverse metabolites viz sesquiterpenes, triterpenes and polyterpenes in the cytoplasm 30 .…”

Section: Discussionmentioning

confidence: 59%

HMG-CoA reductase from Camphor Tulsi (Ocimum kilimandscharicum) regulated MVA dependent biosynthesis of diverse terpenoids in homologous and heterologous plant systems

Bansal

Narnoliya

Mishra

et al. 2018

Sci Rep

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Ocimum kilimandscharicum is unique in possessing terpenoids whereas other Ocimum species are renowned for phenylpropanoids as major constituents of essential oil. The key enzyme of MVA/terpenoid metabolic pathway viz 3-hydroxy-3-methylglutaryl Co-A reductase (OkHMGR) of 1.7-Kb ORF encoding ~60-kDa protein was cloned from O. kilimandscharicum and its kinetic characteristics revealed the availability of HMG-CoA as a control point of MVA-pathway. Transcript profiling of the OkHMGR elucidated tissue-specific functions of the gene in flower and leaf tissues in accumulation of terpenoidal essential oil. OkHMGR was differentially regulated in response to exposure to methyl-jasmonate, salicylic-acid, and stress conditions such-as salt and temperature stress, demonstrating its key role in managing signaling and stress-responses. To elucidate its functional role, OkHMGR was transiently over-expressed in homologous and heterologous plants such as O. sanctum, O. basilicum, O. gratissimum, Withania somnifera and Artemisia annua. The over-expression and inhibition dual strategy revealed that the additional OkHMGR in-planta could afford endogenous flow of isoprenoid units towards synthesis of terpenoids. The present study provides in-depth insight of OkHMGR in regulation of biosynthesis of non-plastidal isoprenoids. This is first report on any gene of MVA/isoprenoid pathway from under-explored Camphor Tulsi belonging to genus Ocimum. Studies also suggested that OkHMGR could be a potential tool for attempting metabolic engineering for enhancing medicinally important terpenoidal metabolites in plants.

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Section: Discussionmentioning

confidence: 59%

HMG-CoA reductase from Camphor Tulsi (Ocimum kilimandscharicum) regulated MVA dependent biosynthesis of diverse terpenoids in homologous and heterologous plant systems

Bansal

Narnoliya

Mishra

et al. 2018

Sci Rep

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“…The ability to synthesize bixin and, thereby, its biosynthetic pathway has been the focus of intense interest in some plants like annatto (Mercadante et al 1997;Mercadante and Pfander 1998;Narváez et al 2001;Jako et al 2002;Bouvier et al 2003;Rodríguez-Ávila et al 2011a, b;Soares et al 2011;Rivera-Madrid et al 2013;Cárdenas-Conejo et al 2015), Costus pictus (Annadurai et al 2012), Crocus sativus, and Vitis vinifera (Ramamoorthy et al 2010). These pigments are commercially known as annatto (E160b), a mixture of bixin (C 25 H 30 O 4 ) as the main color component and traces of norbixin, bixin dimethyl ester, and other minor apocarotenoids.…”

Section: Annatto As a Key Species To Understand Metabolic Pathways Ofmentioning

confidence: 99%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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“…As previously described, terpene biosynthesis, particularly sterols biosynthesis, might be involved in cell differentiation ( Falginella et al., 2021 ). BoHMGR transcript activation was previously documented in fruit development, including that of the seeds in B. orellana ( Narváez et al., 2001 ), prompting the suggestion of a central role in growth and development. No previous reports on BoFPS in B. orellana were found in the literature.…”

Section: Discussionmentioning

confidence: 83%

“…Expression levels were determined in all (S1, S3, and S5) stages of seed development and the leaves of each accession using the TPM normalized metric ( Table S4 ). We selected genes encoding the enzyme 3-hydroxy 3-methylglutaryl Coenzyme A reductase (HMGR), which produces mevalonate and is involved in the early triterpene pathway ( Narváez et al., 2001 ), and the gene encoding farnesyl diphosphate synthase (FFP), which is involved in the first stage of sesquiterpenes (C 15 ) biosynthesis ( Durairaj et al., 2019 ). Seven different HMGR orthologues were identified.…”

Section: Resultsmentioning

confidence: 99%

Gene expression profile during seed development of Bixa orellana accessions varying in bixin pigment

Cárdenas-Conejo

Narváez-Zapata²,

Carballo-Uicab³

et al. 2023

Front. Plant Sci.

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Diverse morphological, cellular and physiological changes occur during seed maturation in Bixa orellana when the seed tissues form specialized cell glands that produce reddish latex with high bixin amounts. Transcriptomic profiling during seed development in three B. orellana accessions (P12, N4 and N5) with contrasting morphologic characteristics showed enrichment in pathways of triterpenes, sesquiterpenes, and cuticular wax biosynthesis. WGCNA allows groups of all identified genes in six modules the module turquoise, the largest and highly correlated with the bixin content. The high number of genes in this module suggests a diversification of regulatory mechanisms for bixin accumulation with the genes belonging to isoprene, triterpenes and carotene pathways, being more highly correlated with the bixin content. Analysis of key genes of the mevalonate (MVA) and the 2C-methyl-D-erythritol-4-phosphate (MEP) pathways revealed specific activities of orthologs of BoHMGR, BoFFP, BoDXS, and BoHDR. This suggests that isoprenoid production is necessary for compounds included in the reddish latex of developing seeds. The carotenoid-related genes BoPSY2, BoPDS1 and BoZDS displayed a high correlation with bixin production, consistent with the requirement for carotene precursors for apocarotenoid biosynthesis. The BoCCD gene member (BoCCD4-4) and some BoALDH (ALDH2B7.2 and ALDH3I1) and BoMET (BoSABATH1 and BoSABATH8) gene members were highly correlated to bixin in the final seed development stage. This suggested a contributing role for several genes in apocarotenoid production. The results revealed high genetic complexity in the biosynthesis of reddish latex and bixin in specialized seed cell glands in different accessions of B. orellana suggesting gene expression coordination between both metabolite biosynthesis processes.

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Differential expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) during flower and fruit development of Bixa orellana

Cited by 14 publications

References 38 publications

HMG-CoA reductase from Camphor Tulsi (Ocimum kilimandscharicum) regulated MVA dependent biosynthesis of diverse terpenoids in homologous and heterologous plant systems

HMG-CoA reductase from Camphor Tulsi (Ocimum kilimandscharicum) regulated MVA dependent biosynthesis of diverse terpenoids in homologous and heterologous plant systems

Somatic Embryogenesis: Fundamental Aspects and Applications

Gene expression profile during seed development of Bixa orellana accessions varying in bixin pigment

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