Differential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide

Breccia, Gabriela; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Picardi, Liliana Amelia; Nestares, G.

doi:10.1016/j.plantsci.2013.03.008

Cited by 20 publications

(26 citation statements)

References 61 publications

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“…Imidazolinone herbicides control weeds by inhibiting the Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/ecoenv action of the acetohydroxyacid synthase (EC 2.2.1.6), also called acetolactate synthase (EC 4.1.3.18). In plants, the enzyme catalyzes the key reactions in the biosynthesis pathways of three branchedchain aliphatic amino acids, i.e., valine, isoleucine, and leucine, with its activity regulated by the end products of the pathways (Breccia et al, 2013;Zhou et al, 2010). This inhibition causes a disruption in protein synthesis, which, in turn, leads to interference in DNA synthesis and cell growth, and eventually to weed death.…”

Section: Introductionmentioning

confidence: 99%

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias

Soloneski

Nikoloff

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Introductionmentioning

confidence: 99%

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias

Soloneski

Nikoloff

et al. 2015

Ecotoxicology and Environmental Safety

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“…As in other plant species, TUB, ACT, and EF1 have been the most frequently employed as reference genes in sunflower (Breccia et al, 2013;Moschen et al, 2014;Ochogavia et al, 2014;Ungerer and Kawakami 2013). However, no detailed validation has been carried out to test the suitability of these genes and novel genes in different sunflower tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Vegetative tissue was obtained from seedlings grown in pots as described Breccia et al (2013) (Schneiter and Miller 1981). Immature and mature disc flowers were collected from the flower bud and male reproductive stages, respectively, as described by Miller (1987).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Further expression studies of ahas genes in cultivars carrying resistant alleles could be useful for the characterization of mechanisms regulating ahas gene expression. However, only a limited number of traditionally used reference genes have been applied as normalizers in this species (Breccia et al, 2013;Fernandez et al, 2011;Ochogavia et al, 2014) and no previous comparison on nontraditional reference genes were reported in this species. The aim of this work was to evaluate the stability of ten genes: five traditionally used and five new putative genes in different sunflower tissues and developmental stages.…”

Section: Introductionmentioning

confidence: 99%

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Identification of suitable reference genes by quantitative real-time PCR for gene expression normalization in sunflower

Ochogavía¹,

Novello²,

Picardi³

et al. 2017

Plant Omics

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Quantitative real-time PCR (qPCR) is currently the most accurate method for detecting differential gene expression, but depends greatly on normalization with stably expressed housekeeping genes. Transcriptomics analyses and experimental validation in different plant species have shown that the reliability of these endogenous controls can be influenced by, growth conditions and organs/tissues examined. Thus, reliable validation of reference genes is required to ensure proper normalization. This paper presents a systematic comparison of ten potential reference genes in sunflower: five commonly used genes (Actin, Elongation Factor1, Plastid-encode RNA polymerase, Tubulin, and Ubiquitin, as ACT, EF1, PEP, TUB, and UBQ respectively), as well as five new candidates (Translation initiation factor, MicroRNA precursors 171 and 156, Ask-interacting protein, and Protein of unknown function, as ETIF5, MIR171, MIR156, SKIP, and UNK2 respectively). Reference gene expression stability was examined by qPCR across 20 biological samples, representing different tissues at various developmental stages. Expression of all 10 genes was variable to some extent, but that of ACT, UNK2, and EF1 was overall the most stable. A combination of ETIF5/UNK2/EF1 would be appropriate to use as a reference panel for normalizing gene expression data among vegetative tissues, whereas the combination of ACT/MIR156/UNK2 is most suitable for reproductive tissues. Reference genes selected in this study were further validated by examining relative expression of ahas1, one of three acetohydroxyacid synthase genes of sunflower. Our identification and validation of suitable normalizer genes will be of use to ensure accurate results in future transcriptomics studies in this crop.

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“…So, molecular approaches include sequencing (Corbett & Tardif, 2006), enable deciphering the genetic basis of resistant (Duran-Prado et al, 2004;Duggleby et al, 2008;Breccia et al, 2013;Ochogavia et al, 2014;Tranel et al, 2014). Therefore, in this study, it was investigated that whether target-site mutation confers resistance to ALS inhibitor herbicide with two active ingredient (mesosulfuron + iodosulfuron) in wild mustard (S. arvensis) or not, and, if so, the resistance levels for each homozygous/heterozygous mutation were also determined.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistance to acetolactate synthase inhibiting herbicide in wild mustard (Sinapis arvensis L.)

Fathabadi¹,

Zand²,

Masumi³

et al. 2016

JEBAS

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To evaluate the mechanism and levels of herbicide resistance in Sinapis arvensis, a series of experiments were performed. Sequencing of ALS gene in S. arvensis sub-populations was carried out and ALS-based resistance (substitution of Asp by Glu) was detected at position 376 in this weed species. Further, it was reported that combined application of herbicide mesosulfuron + iodosulfuron can cause up to 50% reduction in dry weight (GR50) of heterozygous resistant (FHR3), homozygous resistant (FHR2) and homozygous susceptible (MHS1) sub-populations and it was reported 2409, 603 and 289 g ha -1 , respectively. Further, resistance indices (RI) were reported 8.3 and 2 for FHR3 and FHR2 sub-populations, respectively. Based on LD50, RI values were 8.5 and 4.5 for FHR3 and FHR2, respectively. Growth reduction occurred in the resistant homozygous when compared with the heterozygous sub-population in the presence of mesosulfuron + iodosulfuron.

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Differential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide

Cited by 20 publications

References 61 publications

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Identification of suitable reference genes by quantitative real-time PCR for gene expression normalization in sunflower

Evaluation of resistance to acetolactate synthase inhibiting herbicide in wild mustard (Sinapis arvensis L.)

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